长叶红砂为内蒙古东阿拉善-西鄂尔多斯地区特有珍稀泌盐,强旱生小灌木,对盐渍荒漠环境具有极强适应性。利用RT-PCR和RACE技术从该植物中分离出质膜Na+/H+逆向转运蛋白基因(RtSOS1),该cDNA全长为3 829bp,开放阅读框为3 438 bp,编码一个含1 145个氨基酸的蛋白质,推测分子量为126.76 kDa。氨基酸序列的生物信息学分析推测,该蛋白N端含有11个跨膜结构域,C端为一个长约700个氨基酸的亲水性尾,具有磷酸化和自我抑制结构域。同源性比对和系统发育分析证实,RtSOS1与其他植物的质膜Na+/H+逆向转运蛋白亲缘关系较近。
Reaumuria trigyua Maxim. is an endangered small shrub with the features of a recretohalophyte. This species is endemic to the Eastern Alxa Western Ordos area and developed distinctive strategies to adapt to the semidesert and salty soil environment. A full}ength cDNA of the plasma Na+/H+antiporter (RtSOSl)was isolated from this species by using RT-CR and RACE technologies. The 3 829 by sequence comprised a 3 438 by open reading frame，encoding an 1 145 amino acids protein with the molecular weight of 126.76 kDa. Bioinformatics analyze reveals that RtSOSl composed of 11 transmembrane domains within its N terminal portion，and a hydrophilic cytoplasmic tail with the length approximately 700 amino acids in its Germinal portion. In the Germinal region，the phosphorylation domain and the auto-nhibited domain are found. The Homology comparison and phylogenetic analysis showed that RtSOSl is related to plasma membrane Na+/H+antiporter in other plant species.
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