论文

长片段小麦细菌人工染色体DNA亚克隆文库的构建

  • 高双成 ,
  • 王世华 ,
  • 施江 ,
  • 孔祥生 ,
  • 史国安
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  • 河南科技大学, 农学院, 洛阳市牡丹生物学重点实验室, 河南洛阳 471003
高双成(1976-),男,山西夏县人,讲师,主要从事植物分子生物学研究.

收稿日期: 2009-02-23

  网络出版日期: 2014-10-14

基金资助

河南科技大学人才科学研究基金(09001163);河南科技大学科学研究基金(2008zy038)

Construction of Subclone Library for Wheat Megabase BAC DNA

  • GAO Shuang-cheng ,
  • WANG Shi-hua ,
  • SHI Jiang ,
  • KONG Xiang-sheng ,
  • SHI Guo-an
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  • Agricultural College, Henan University of Science and Technology, Luoyang Key Laboratory of Tree Peony Biology, Luoyang 471003, China

Received date: 2009-02-23

  Online published: 2014-10-14

摘要

为了加快小麦基因组的测序,以Langdon库中的一个BAC克隆(BAC790O10)为材料,利用HydroShear DNA剪切仪将其打断,产生许多大小不同的DNA随机片段,回收其中~5 kb的片段,并将这些片段随机克隆入TOPO载体,构建了适合于鸟枪法测序的亚克隆文库.

本文引用格式

高双成 , 王世华 , 施江 , 孔祥生 , 史国安 . 长片段小麦细菌人工染色体DNA亚克隆文库的构建[J]. 华北农学报, 2009 , 24(4) : 23 -25 . DOI: 10.7668/hbnxb.2009.04.005

Abstract

BAC subclone library is an important platform for sequencing the whole genome or some specific regions. In order to accelerate the process of sequencing the wheat genome, a BAC clone (BAC790010) fromLangdon librarywas selected as material, and then was broken by DNA shearing instrument to produce many DNA fragments with different sizes, fromwhich the 3- 5 kb DNA fragments were retrieved and cloned to TOPO vector and then the subclone librarywas constructed that was suitable for shotgun sequencing. This BAC subclone librarywould provide a platformfor gene cloning and genome research of wheat.

参考文献

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