用山东莱西市玉米弯孢叶斑病菌强致病类型菌株QN-10,接种玉米抗病自交系齐319和感病自交系7922,采用蛋白质组技术分析其差异蛋白及功能。结果表明,齐319感染弯孢菌3 d后,有12个差异蛋白质斑点被诱导合成,8个为增量表达蛋白质斑点,2个为减量表达蛋白质斑点,2个为新产生蛋白质斑点|自交系7922有3个蛋白质斑点表达量增加,2个斑点表达量下降,但没有发现新的蛋白质合成。齐319差异蛋白质斑点鉴定表明,分别属于β-1,3-葡聚糖酶、F-box亮氨酸高度重复蛋白、锌指蛋白。
The protein of resistant corn variety QI 319 and susceptible variety 7922 was extracted from leaves and separated using two-dimensional polyacrylamidgel electrophoresis(2-D)at 3d after inoculating prevalent race QN-10 of Curvularia lunata. In QI 319,compared with control with no pathogen inoculation,12 novel proteins were induced;In 7922,three protein spots of and two protein spots of showed increase and decrease in protein abun-dance,respectively,but there were no hovel protein spots induced. Among the 12 hovel protein spots induced in QI 319,six spots were identified using MALDI-TOF-MS and NCBI database searching,which were F-box and leucine-rich repeat protein,heavy metal transport/detoxification protein,beta-1,3-glucanase and zinc finger protein.
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