论文

水稻淀粉合成相关基因对稻米品质效应的研究

  • 陈峰 ,
  • 孙公臣 ,
  • 杨泽峰 ,
  • 张士永 ,
  • 张洪瑞 ,
  • 杨亚春 ,
  • 袁守江 ,
  • 张正球
展开
  • 1. 山东省水稻研究所,山东 济宁 272177;
    2. 扬州大学 教育部植物基因组学;
    3. 安徽省农科院 水稻所, 安徽 合肥 230031
陈峰( 1979- ), 男, 山东曲阜人, 助理研究员, 硕土, 主要从事水稻遗传育种研究.

收稿日期: 2008-12-25

  网络出版日期: 2014-10-14

基金资助

山东省农科院青年基金(2006YQN006);山东省农科院创新基金(2006YCX009,2007YCX007);山东省农业良种工程项目(2007L2006-02);江苏省高校重点实验室开放课题(K06001)

The Effects of Starch-synthesizing Genes on Rice Quality

  • CHEN Feng ,
  • SUN Gong-chen ,
  • YANG Ze-feng ,
  • ZHANG Shi-yong ,
  • ZHANG Hong-rui ,
  • YANG Ya-chun ,
  • YUAN Shou-jiang ,
  • ZHANG Zheng-qiu
Expand
  • 1. Shandong Rice Research Institute, Jining 272177, China;
    2. The Key Laboratory for Plant Functional Genomics, Ministry of Education, Agronomy College, Yangzhou Univisity, Yangzhou 225009, China;
    3. Rice Research Institute, Anhui Academy of Agriculture Sciences, Hefei 230031, China

Received date: 2008-12-25

  Online published: 2014-10-14

摘要

颗粒淀粉合成酶(GBSS)、淀粉分支酶1(SBE1)和淀粉分支酶3(SBE3)是淀粉合成过程中的3个关键酶,这3个酶主要由WxSbelShe3 3个基因控制.设计这3个基因位点的分子标记检测了183个水稻品种的基因型,分析了3个基因对稻米理化品质和RVA谱特征的效应.结果表明:3个分子标记均能很好的区分3个位点上等位基因的籼梗来源,根据3个位点的基因型可将183个品种分为8种类型;单个基因遗传效应分析表明,不同基因型品种间淀粉的理化特性(AC、GC、RVA)存在显著或极显著差异,3个基因的联合效应在不同基因型组合间也存在显著的差异.Wx基因对大多数品质性状效应显著,She3效应次之,Sbel效应较小;SbelSbe3基因在不同的Wx等位基因背景下,对稻米的品质的理化特征的效应不同.3基因间存在互作效应,WxSbe3的互作效应较大.

本文引用格式

陈峰 , 孙公臣 , 杨泽峰 , 张士永 , 张洪瑞 , 杨亚春 , 袁守江 , 张正球 . 水稻淀粉合成相关基因对稻米品质效应的研究[J]. 华北农学报, 2009 , 24(2) : 55 -59 . DOI: 10.7668/hbnxb.2009.02.012

Abstract

Granule-bound starch synthase (GBSS),Starch branching enzyme 1 (SBE1) and Starch branching enzyme 3(SBE3) are three major enzymes involved in starch biosynthesis in rice endosperm. The granule-bound starch synthase was encoded by the Wx gene,and the starch branching enzymes were encoded by the Sbe1 and Sbe3 gene.And molecular markers for Sbe1,Sbe3 developed based on sequence diversities between the rice subspecies indica and japonica,together with PCR2AccⅠmarker for Wx gene were used to investigate the genotypes of 183 rice cultivars.The result showed that the developed molecular markers for Wx,Sbe1,Sbe3 could distinguish indica or japonica alleles at three loci.Among all the 183 rice cultivars,eight genotype combinations were observed at Sbe1,Sbe3 and Wx loci. In order to explore the genetic effects of three genes,ANOVA and multiple comparison analysis were conducted. The results showed that rice cultivars with different genotypes exhibited different phenotypes,including amylose content,gel consistency and some of RVA characteristics,and the significant differences were observed. And the genetic effects of indica and japonica alleles at three gene loci were different,Wx gene had significant effects on mainly rice quality traits,followed by Sbe3 and Sbe1. The genetic effects of Sbe1 and Sbe3 in different backgrounds (Wxa, Wxb)were different. Interactions existed among Wx, Sbe1, Sbe3,Interaction was generally detected between Wx and Sbe3.

参考文献

[1] 李欣,顾铭洪,潘学彪.常见水稻品种稻米品质的研究[J].江苏农学院学报,1987,8(1):1-8.
[2] Sano Y.Differential regulation of waxy gene expression in rice endosperm[J].Theoretical and Applied Genetics,1984,68:467-473.
[3] Hirano H Y,Eiguchi M,Sano Y.A single base change altered the regulation of the Waxy gene at the posttranscriptional level during the domestication of rice[J].Molecular Biology and Evolution,1998,15(8):978-987.
[4] Wang Z Y,Zbeng F Q,Shen G Z,et al.The amylose content in rice endosperm is related to the post-transcriptionul regulation of the Waxy gene[J].The Plant Journal,1995,7(4):613-622.
[5] Wanchana S,Toojinda T,Tragoonrung S,et al.Duplicated coding sequence in the waxy allele of tropical glutinous rice (Oryza sativa L.)[J].Plant Science,2003,165:1193-1199.
[6] 吴洪恺,梁国华,严长杰,等.水稻不同生态型品种间直链淀粉含量的变异及其遗传分析[J].作物学报,2006,32(9):1301-1305.
[7] Han X Z,Hamaker B R.Amylopectin fine structure and rice starch paste breakdown[J].Journal of Cereal Science,2001,34:279-284.
[8] Ong M H,Blanshard J M V.Texture determinants of cooked,parboiled riee.2.Physicochemical properties and leaching behavior of rice[J].Journal of Cereal Science,1995,21:261-269.
[9] Martin C,Smith A M.Starch biosynthesis[J].Plant Cell,1995,7:971-985.
[10] Okagaki R J,Wessler S R.Comparison of non-mutant and mutant waxy genes in rice and maize[J].Genetics,1988,120:1137-1143.
[11] Yamanouchi H,Nakamura Y.Organ specificity of isoforms of starch branching enzyme (Q-enzyme) in rice[J].Plant Cell Physiology,1992,33:985-991.
[12] Mizuno K,Kawasaki T,Shimada H,et al.Alteration of the structural properties of starch components by the lack of an isoform of starch branching enzyme in rice seeds[J].Journal of Biological Chemistry,1993,268:19084-19091.
[13] Han Y P,Xu M L,Liu X Y,et al.Gene coding for starching enzymes are major contributors to starch viscosity characteristics in waxy rice (Oryza sativa L.)[J].Plant Science,2004,166:357-364.
[14] 蔡秀玲,刘巧泉,汤述翥,等.用于筛选直链淀粉含量为中等的籼稻品种的分子标记[J].植物生理与分子生物学报,2002,28(2):137-144.
[15] 严长杰,田舜,张正球,等.水稻栽培品种淀粉合成相关基因来源及其对品质的影响[J].中国农业科学,2005,39(5):865-871.
[16] Nakamura Y.Towards a better understanding of the metabolic system for amylopectin biosynthesis in plants:rice endosperm as a model tissue[J].Plant Cell Physiology,2002,43(7):718-725.
[17] 吴洪恺,梁国华,顾燕娟,等.水稻淀粉合成相关基因对稻米RVA谱特征的影响[J].作物学报,2006,32(11):1597-1603.
[18] 陈峰,张正球,张士永,等.稻米淀粉的生物合成与品质改良的研究进展[J].山东农业科学,2008(3):20-25.
文章导航

/