为探讨小麦种子休眠解除的机制,利用蛋白质组学方法比较解除休眠前后的小麦胚在ABA和H2O2中的蛋白质表达情况.H2O2能打破种子休眠,而ABA则使种子保持休眠状态.比较2D图谱后,利用MALD1-TOF-MS鉴定了10个在不同处理间表达丰度差异显著的蛋白点,其中一些蛋白在已知的种子萌发中起重要作用.种子休眠的解除是一个复杂的发育过程,涉及环境胁迫反应(胚发育后期丰度蛋白、NAD(P)H脱氢酶)、细胞循环(生长素反应蛋白)、信号转导(钙调素相关蛋白)和贮藏蛋白(2S种子清蛋白)代谢等生理活动.为研究H2O2打破小麦休眠的分子机理提供了重要参考.
A proteomic approach was used to analyze the mechanisms of dormancy breaking in wheat embryos and the participation of hydrogen peroxide (H2O2) and abscisic acids (ABA) in this process. The dormant and non-dormant embryos were dissected and treated with water,1 % H2O2 and 25μmol/ L ABA solution. ABA delayed,whereas H2O2 promoted seed dormancy breaking. 2D maps for water,ABA and H2O2 were established. Ten spots,showing significant changes in expression level,were identified by MALDI2TOF2MS. Some of them had previously been shown to play a role during dormancy breaking or germination in several plant species.We conclude that the mechanism of seed dormancy breaking is acomplex developmental processes,involves in environment stress (such as LEA,NAD(P) H dehydrogenase),cell cycle activity(auxin-responsive protein IAA19),signal transduction (calmodulin2related protein) and seed storage protein (2S albu-min-like protein),etc.Altogether,the results provided important information on the molecular mechanisms of breaking dormancy in the wheat seeds by H2O2 application.
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