论文

小鼠催乳素释放肽的初步克隆

  • 朱河水 ,
  • 都军霞 ,
  • 王新卫 ,
  • 王月影 ,
  • 王艳玲
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  • 河南农业大学, 动物生理生化实验室, 河南, 郑州, 450002
朱河水(1975-), 男, 河南确山人, 博士生, 研究方向为动物生理学

收稿日期: 2007-09-20

  网络出版日期: 2014-10-14

基金资助

国家自然科学基金(30671531)

Primary Cloning of Mouse Prolactin-releasing Peptide cDNA

  • ZHU He-shui ,
  • DU Jun-xia ,
  • WANG Xin-wei ,
  • WANG Yue-ying ,
  • WANG Yan-ling
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  • Lab of Animal Physiology & Biochemistry Henan Agricultural University, Zhengzhou 450002, China

Received date: 2007-09-20

  Online published: 2014-10-14

摘要

为克隆并分析小鼠催乳素释放肽基因, 从小鼠下丘脑提取总RNA, 进行RT-PCR, PCR产物与pMD-19T连接后转化E.coli DH5α, 检测阳性克隆并测序。测序结果表明, 所得序列与大鼠催乳素释放肽编码区序列的相似指数为86.3%。克隆所得序列为小鼠催乳素释放肽的基因片段。

本文引用格式

朱河水 , 都军霞 , 王新卫 , 王月影 , 王艳玲 . 小鼠催乳素释放肽的初步克隆[J]. 华北农学报, 2007 , 22(6) : 199 -200 . DOI: 10.7668/hbnxb.2007.06.041

Abstract

To clone and analyse mouse prolactin-releasing peptide. Total RNA was extracted from hypothalamus and amplified by RT-PCR. The PCR products were ligated into the pMD-19T vector, and then transformed into competent cells of E. coli DH5a, The positive cloning was sequenced and anelyzed.The cloned nucleotide sequence shared 86.3% hourology with the code zone sequence of rat prolactin-releasing peptide. The cloned nucleotide sequence was pert of mouse polactin-releasing peptide gene.

Key words: Mouse; PrRP; Cloning; Sequence

参考文献

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