论文

猪沙波病毒CH430株p70基因克隆与生物信息学分析

  • 刘伟 ,
  • 王恩丽 ,
  • 柳纪省 ,
  • 杨彬 ,
  • 兰喜
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  • 1. 中国农业科学院 兰州兽医研究所, 家畜疫病病原生物学国家重点实验室, 草食动物疫病重点开放实验室, 甘肃 兰州 730046;
    2. 甘肃农业大学 动物医学院, 甘肃 兰州 730070
刘伟(1986-),女,河北唐山人,硕士,主要从事动物传染病分子生物学研究。

收稿日期: 2014-03-02

  网络出版日期: 2014-09-19

Cloning and Bioinformatics Analysis of Porcine Sapovirus CH430 Non-structural Protein p70 Gene

  • LIU Wei ,
  • WANG En-li ,
  • LIU Ji-xing ,
  • YANG Bin ,
  • LAN Xi
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  • 1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases, Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;
    2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2014-03-02

  Online published: 2014-09-19

摘要

利用RT-PCR方法从猪沙波病毒CH430株中克隆出非结构蛋白p70基因并测序,结果显示,p70基因全长1 995 nt,编码665个氨基酸,经同源性比对及遗传进化树分析表明,CH430株为基因Ⅲ型沙波病毒毒株。生物信息学分析显示该蛋白理论等电点(pI)为5.96,理论分子质量为72 876.6 u;其序列上共发现25个磷酸化位点,分别为Ser(6)、Thr(13)、Tyr(6),而蛋白的磷酸化与信号传导有关,预测该蛋白为一重要的信号传导分子;无信号肽和跨膜区;该蛋白含有胰蛋白酶样丝氨酸蛋白酶和RdRp功能域;胰蛋白酶样丝氨酸蛋白酶功能域核心结构为1个由7个β折叠形成柱状结构和1个由1个α螺旋和7个β折叠扭曲平行形成1个不完全的桶状结构共同组成,RdRp功能域核心结构为1个具有3个子结构域空间结构,该结构与右手掌在形态上非常相似,而3个子结构域也被认为是手指、手掌和拇指。

本文引用格式

刘伟 , 王恩丽 , 柳纪省 , 杨彬 , 兰喜 . 猪沙波病毒CH430株p70基因克隆与生物信息学分析[J]. 华北农学报, 2014 , 29(3) : 46 -51 . DOI: 10.7668/hbnxb.2014.03.010

Abstract

The non-structural protein p70 gene of PoSaV CH430 strain was cloned by RT-PCR and then sequenced in our study.The results showed that the full length of p70 gene was 1 995 nt encoding a protein of 665 aa.Homology alignment and evolutionary tree showed that PoSaV CH430 strain belong to GⅢ.The bioinformatics analysis demonstrated that the isoelectric point and molecular weight of non-structural protein p70 were 5.96 and 72 876.6 u.The protein had no signal peptide and transmenbrane domain.There were 25 phosphorylation sites including 6 Sers, 13 Thrs and 6 Tyrs.Protein phosphorylation was concerned with signal transduction, so this protein may be a signaling molecule.There were Trypsin-like serine proteases and RdRp functional domain.Main structure of trypsin-like serine proteases functional domain was a column structure and an incomplete β-barrel.Main structure of RdRp functional domain was a fold whose organisation has been likened to the shape of a right hand with three subdomains termed fingers, palm and thumb.

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