摘要： 为明确淡紫拟青霉PL-HN-16促进植物生长的活性因子.采用Salkowski比色法、胚芽鞘法和高效液相色谱(HPLC)分析培养物中生长素(IAA);离子柱层析和生物活性跟踪法,明确其促植物生长活性因子.Salkowski比色、小麦胚芽鞘试验和HPLC试验结果表明:PL-HN-16不产生IAA;柱层析和活性跟踪结果表明,其促生长活性因子为分子量约14 kDa的蛋白,当蛋白浓度低于0.10 μg/mL时,促生长作用呈明显剂量相关性,对菜心生长促进率达13.15%,高于0.10 μg/mL抑制菜心生长.PL-HN-16促植物生长因子为分子量约14 kDa的蛋白,有关植物外源激素样功能蛋白研究鲜有报道,开展其研究具有理论和应用价值.

关键词: 淡紫拟青霉, 类植物生长素, 促进生长, 分离纯化

Abstract: To determine the main factor of promoting plant growth from Paecilomyces lilacinus PL-HN-16.Salkowski colorimetric test,wheat coleoptiles test and HPLC were used to detect IAA.Activities of the protein were test which was separed by ion exchang chromatography from the culture bath.Results of Salkowski colorimetric test,wheat coleoptiles test and HPLC test showed that strain PL-HN-16 did not produce IAA.The strain could increase the soluble phosphorus but it was not the main reason to promote plant growth.Protein test results showed that the protein which molecular weight was about 14 kDa could promote plant growth when the content below 0.10 μg/mL and dose-relative positive correlation,the percent of promoting was increased by 13.15%.The protein might inhabit plant growth when the content was higher than 0.10 μg/mL.The protein which MW was about 14 kDa produced by the strain PL-NH-16 was the factor of promoting plant growth.There was not any report about heterologous protein affected as phytohormone IAA to plant.

Key words: PL-HN-16, Plant growth regulators, Promoting plant growth, Purification

中图分类号: 

• S432.4