摘要： Effects of five incubation temperatures (25℃,28℃,31℃,35℃ and 37℃ for 24 hours) and four incubation time periods (0.4,16 and 24 hours at 35℃) on isolated microspore culture of Chinese cabbage were studied.The results showed that cultured microspores of Chinese cabbage developed into embryos at all the incubation temperatues from 28℃ to 37℃,but the best response to high temperature occured at 35℃.Among the four kinds of time periods,the highest yield of embryos was obtained at the 24h treatment.Therefore,the isolated microspore culture of Chinese cabbege ran be efficiently carried out at 35℃ for 24 hours.
关键词:
Chinese cabbage,
isolated microspore culture temperature,
embryo
高睦枪, 栗根义. Effects of different high temperatures and incubation time periods on embryogenesis in isolated microspore culture of Chinese cabbage (Brassica campestris L.ssp pekinensis)[J]. 华北农学报, 1994, 9(S2): 16-18. doi: 10.7668/hbnxb.1994.S2.004.
Gao Muqiang, Li Genyi. [J]. ACTA AGRICULTURAE BOREALI-SINICA, 1994, 9(S2): 16-18. doi: 10.7668/hbnxb.1994.S2.004.