生长分化因子11(Growth and differentiation factor-11,简称GDF11) 是BMP/TGF-β超家族一员,编码一种在早期胚胎发育过程中起重要作用的骨形态发生蛋白,在确立骨骼模式中起重要作用。本研究旨在克隆里岔黑猪GDF11基因部分cDNA,并检测其在不同组织中的表达差异。结果表明,所克隆的猪部分GDF11基因序列为1 161 bp(GenBank:HQ657211),编码332个氨基酸,与人、小鼠、大鼠、牛、马、兔、斑马鱼GDF11氨基酸序列同源性分别为99.10%,99.10%,99.10%,99.40%,99.10%,98.80%,78.82%。荧光定量PCR分析结果显示,GDF11基因mRNA在14种组织中均有表达,总体趋势为:脊椎>背膘>膀胱>肺>脾>胆囊>肾>大肠>心>骨骼肌>肝>小肠>眼肌>胃。这为进一步研究里岔黑猪GDF11基因的结构和功能及其脊椎数发生的机理奠定了基础。
GDF11, also known as bone morphogenetic protein 11(BMP11), is a member of the transforming growth factor-β superfamily and BMPs subfamily. It is a secreted protein which is considered to be a key regulator of axial skeleton patterning during early embryogenesis. In this study, the partial cDNA of porcine GDF11 gene was cloned by RT-PCR and the expression patterns of GDF11 gene were detected. Sequence analysis indicates that the sequences of porcine GDF11 gene were 1161 bp in length(GenBank: HQ657211. 2). The cloned cDNA encoded a protein of 332 amino acid residues, which shared 99. 10%, 99. 10%, 99. 10%, 99. 40%, 99. 10%, 98. 80% and 78. 82% identity with those of human, mouse, rat, cattle, horse, rabbit and zebra fish, respectively. Expression patterns of GDF11 were detected in 14 different tissues by real time quantitative RT-PCR, and patterns of GDF11 gene, as a whole, tended to be spinal cord>backfat>urinary bladder>lung>spleen>gallbladder>kidney>large intestine>heart>skeletal muscle>liver>small intestine>longissimus Dorsi muscle>stomach. These results provide important information for further elucidating the molecular biology functions and mechanism of GDF11 gene.
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