论文

植物病原菌群体猝灭基因克隆及诱导表达载体构建

  • 欧阳乐军 ,
  • 黄真池 ,
  • 沙月娥 ,
  • 陈良 ,
  • 谢先荣 ,
  • 曾富华
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  • 1. 湛江师范学院生命科学与技术学院, 广东湛江 524048;
    2. 湖南农业大学生物科学与技术学院, 湖南长沙 410128
欧阳乐军(1976- ), 男, 湖南南县人, 助理研究员, 博士, 主要从事植物抗病基因工程研究。

收稿日期: 2011-11-06

  网络出版日期: 2014-10-14

基金资助

国家星火计划项目(2011GA780067);广东省自然科学基金(10152404801000005;10452404801004328);广东省科技攻关项目(2010B020301011);湛江市科技攻关项目(2011C3104010)

Cloning and Plant Expression Vector Construction of Quorum Sensing Gene of Plant Bacterial Pathogens

  • OUYANG Le-jun ,
  • HUANG Zhen-chi ,
  • SHA Yue-e ,
  • CHEN Liang ,
  • XIE Xian-rong ,
  • ZENG Fu-hua
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  • 1. Life Science and Technology School, Zhanjiang Normal University, Zhanjiang 524048, China;
    2. College of Bioscience and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2011-11-06

  Online published: 2014-10-14

摘要

N-酰基高丝氨酸内酯是革兰氏阴性细菌致病过程中的关键信号分子,芽孢杆菌中广泛存在的aiiA基因编码AHL-Lactonase能够水解信号分子AHLs。研究利用PCR方法从枯草芽孢杆菌中克隆了aiiA基因,序列分析表明,该基因由753个碱基组成,编码含有250个氨基酸残基的蛋白质,核苷酸序列与已报道aiiA的同源性为87%~96%。将该基因置于诱导型启动子PPP3调控下,连接到植物表达载体pCAMBIA1301中,成功构建了aiiA基因的植物诱导表达载体pCAM-PPP3-aiiA,为进一步通过转基因技术研究该基因的功能奠定了基础。

本文引用格式

欧阳乐军 , 黄真池 , 沙月娥 , 陈良 , 谢先荣 , 曾富华 . 植物病原菌群体猝灭基因克隆及诱导表达载体构建[J]. 华北农学报, 2012 , 27(1) : 18 -23 . DOI: 10.3969/j.issn.1000-7091.2012.01.004

Abstract

N-acyl-homoserine lactones(AHLs) is crucial signal molecule when the gram-negative bacteria infects hose. The AHL-Lactonase, expressed by aiiA gene which widespread in Bacillus sp, can hydrolyze AHLs. The study have cloned the aiiA gene from Bacillus subtilis by PCR. The sequence analysis indicated that the clone was consisted of 751 nucleotides(nt), coding 250 amino acids. The nucleotide sequence showed 87%-96% identities with those of the aiiA gene that have reported. A plant expression vector of the aiiA gene was constructed and named Pcamb-PPP3-aiiA, in which the aiiA was controlled by the PPP3 promoter. This work laid the foundations for future transgenic research on aiiA gene function.

参考文献

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