论文

干旱胁迫下茉莉酸甲酯对水稻叶片质膜透性及无机离子含量的影响

展开
  • 1. 华南农业大学,农业部生态农业重点开放实验室, 广东广州 510642;
    2. 湖北第二师范学院,化学与生命科学学院, 湖北武汉 430205
董桃杏(1982-),女,湖北黄冈人,硕士,主要从事生态学的研究.

收稿日期: 2009-08-08

  网络出版日期: 2014-10-14

基金资助

教育部博士点基金资助项目(2000065402)

Effects of Methyl Jasmonate on Membrane Permeability and Inorganic Ions Content in Rice Leaves under Drought Stress

Expand
  • 1. Key Laboratory of Ecological Agriculture of Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China;
    2. College of Chemistry and Life Science, Hubei University of Education, Wuhan 430205, China

Received date: 2009-08-08

  Online published: 2014-10-14

摘要

茉莉酸甲酯(MeJA)是植物体内一种具有应答外界刺激,传导逆境信号及启动抗逆基因的天然生理活性物质,提高作物的抗旱性是MeJA的重要生理功能之一.研究了干旱胁迫下施用不同浓度(250,25,0.25μmol/L)MeJA对水稻幼苗的生理调控作用.结果表明,干旱处理后水稻叶片水势和叶绿素含量显著下降,叶片MDA含量、电导率及无机离子(K+,Ca2+,Mg2+)含量均显著上升,而喷施不同浓度的MeJA 则显著提高叶片水势和叶绿素含量,降低质膜透性和叶片无机离子含量,从而增强水稻幼苗的抗旱性.以0.25μmol/L MeJA处理抗旱效果最好,25μmol/L次之,然后是20 μmol/L.

本文引用格式

董桃杏, 蔡昆争, 曾任森 . 干旱胁迫下茉莉酸甲酯对水稻叶片质膜透性及无机离子含量的影响[J]. 华北农学报, 2010 , 25(1) : 136 -140 . DOI: 10.7668/hbnxb.2010.01.027

Abstract

Methyl Jasmonate(MeJA)is an important naturally physiological substance that regulates the expression of plant defense genes in response to various environmental stress including drought.Different concentration of MeJA treatment(250,2.5 and 250μmol/L) under drought stress were chosen to study its effect on physiological traits of rice seedlings.Under drought stress condition,leaf water potential and chlorophyll content were significantly decreased,whereas conductivity,the content of MDA and inorganic ions(K+,Ca2+,Mg2+) in rice leaves were significantly increased,these effects could be restrained after spraying various concentration of MeJA(250,2.5,0.25μmol/L),which meant MeJA could increase drought resistance.There existed concentration effect,0.25 μmol/L MeJA had the best effect,2.5 μmol/L took second place,the third was 250μmol/L.

参考文献

[1] Harms K,Atzorn R,Brash A,et al.Expression of a flax allene oxide synthase cDNA leads to increased endogenous jasmonic acid levels in transgenic potato plants but not to a corresponding activation of JA-responding genes[J].Plant Cell,1995,7:1645-1654.
[2] Lehmann J,Atzorn R,Bruckner C,et al.Accumulation of jasmonate,abscisic acid,specific transcripts and proteins in osmotically stressed barley leaf segments[J].Planta,1995,197:156-162.
[3] 蔡昆争,董桃杏,徐涛.茉莉酸类物质(JAs)的生理特性及其在逆境胁迫中的抗性作用[J].生态环境,2006,15(2):397-404.
[4] Creelmn R A,Mulent J E.Jasmonic acid distribution and action in plants:Regulation during development and response to biotic and abiotic stress[J].Proceedings of the National Academy of Sciences of the USA,1995,92:4114-4119.
[5] Moons A,Prinsen E,Bauw G,et al.Antagonistic effects of abscisic acid and jasmonates on salt stress-inducible transcripts in rice roots[J].The Plant Cell,1997,9:2243-2259.
[6] Gao X P,Wang X F,Lu Y F,et al.Jasmonic acid is involved in the water-stress-induced betaine accumulation in pear leaves[J].Plant Cell and Environment,2004,27:497-507.
[7] Gonzaliez A,Tiznado H M,Zavaleta G R,et al.Methyl jasmonate treatments reduce chilling injury and activate the defense response of guava fruits[J].Biochemical and Biophysical Research Communications,2004,313:694-701.
[8] Thaler J S,Owen B,Higgns V J.The Role of the Jasmonate response in plant susceptibility to diverse pathogens with a range of lifestyles[J].Plant Physiology,2004,135(1):530-538.
[9] 潘瑞炽,古焕庆.茉莉酸甲酯对花生幼苗生长合抗旱性的影响[J].植物学报,1998,40(3):256-262.
[10] 姚允聪,张大鹏,王有年.MJ提高苹果幼树抗旱性的研究初报[J].园艺学报,1999,26(5):333-334.
[11] Bandurska H,Stronski Troicski A,Jan K.The effect of jasmonate on the accumulation of ABA,proline and its influence on membrane injury under water deficient in two barley genotypes[J].ACTA Physiologiae Plantarum,2003,25(3):279-285.
[12] 李合生.植物生理生化实验原理与技术[M].北京:高等教育出版社,2000.
[13] 王洪春.植物抗逆生理[J].植物生理学通讯,1981,(6):72-81.
[14] 陈少裕.膜脂过氧化与植物逆境胁迫[J].植物学通报,1989,6(4):211-217.
[15] 陈培琴,郁松林,詹妍妮,等.茉莉酸对葡萄幼苗耐热性的影响[J].石河子大学学报,2006,24(1):87-91.
[16] 董桃杏,蔡昆争,张景欣,等.茉莉酸甲酯对水稻幼苗的抗旱生理效应[J].生态环境,2007,16(4):1261-1265.
[17] 李春香,王玮,李德全.长期水分胁迫对小麦生育中后期根叶渗透调节物质的影响[J].西北植物学报.2001,21(5):924-930.
[18] 蔡昆争,吴学祝,骆世明.不同生育期水分胁迫对水稻根叶渗透调节物质变化的影响[J].植物生态学报,2008,32(2):491-500.
[19] 蔡昆争,吴学祝,骆世明.抽穗期不同程度水分胁迫对水稻产量和根叶渗透调节物质的影响[J].生态学报,2008,28(12):6148-6158.
[20] 关军锋,李广敏.Ca~(2+)与植物抗旱性的关系[J].植物学通报,2001,18(4):473-478.
[21] 蒋明义,郭少川.水分亏缺诱导的氧化胁迫和植物的抗氧化作用[J].植物生理学通讯,1996,32(2):17-23.
[22] 宗会.干旱胁迫下Ca~(2+)·CaM信使系统对稻苗保护酶活性的影响[J].西北植物学报,2004,24(5):843-849.
[23] Nicola H,Evans.Modulation of guard cell plasma membrane potassium currents by methyl jasmonate[J].Plant Physiology,2003,131:8-11.
文章导航

/