采用形态标记和SSR分子标记相结合的方法,对 13个野生扁蓿豆居群的单位面积分枝数、花序花朵数、单枝花序数、花序结荚数、花序种子数、单荚种子数和种子千粒重等种子产量性状的遗传变异进行了研究.结果表明,扁蓿豆野生居群间种子产量性状的变异为 19.97%～109%,种子产量变异最大,变幅为 0.08～11.208 kg/hm~2 (P<0 0);种子千粒重的变异最小,差异不显著(P>0.0),而其他性状差异均显著,根据形态性状聚类结果显示:13个野生居群可分成4大类群;SSR分子标记分析表明:各居群的遗传距离变异范围为1.00～0.11,可见扁蓿豆野生居群间的种子产量性状具有丰富的遗传多样性,经UPGMA方法建立的树状图,可将13个野生居群划分为4大类群.两种标记结果部分一致.

Medicago ruthenica is an important legume forage in China.Genetic diversity was studied in 13 Medicago ruthenica populations by morphological and SSR markers of seed yield characteristics,such as number of twigs in unit area,number of raceme on flowers,number of racemes on a twig,number of pods on a raceme,number of seeds on a raceme,number of seeds on a pod and 1 000 seeds weight.The results showed that genetic variance in seed yield characteristics was from 19.97% to 109%.The variance percentage range of seed yield was the biggest,which the variance scale was from 0.08 to 11.208 kg/ha(P<0.05).The variance of 1 000 seeds weight was the smallest and not significant(P>0.05).Based on the morphological data,13 Medicago ruthenica populations of wild were clustered into 4 groups.The variance of range genetic distance was from 1.00 to 0.11 in different varieties of Medicago ruthenica using SSR.Genetic diversity of seed yield characteristics was rich in different varieties of Medicago ruthenica.The UPGMA Dendrogram displayed that the 13 Medicago ruthenica populations were clustered into 4 groups.