以163份来自于中国、日本、韩国、不丹、尼泊尔和越南6国的各种类型小豆种质资源的大样本群体为材料,利用27条RAPD引物检测该群体得到的261个等位基因数据构建成"总群体",采用完全随机抽样方法,研究不同样本容量(样本位点数)与系统遗传相似系数(Jaccard系数)变化的关系,并进一步配置出不同引物数量、多态位点与Jaccard系数的变异系数曲线方程,通过分析回归曲线的相应切点,确定在一定精度下适合的引物数量及多态位点数.研究结果表明:不同引物数量之间的Jaccard系数的标准差、平均数、变异系数差异极显著. 不同引物数量、不同多态位点与Jaccard系数的变异系数、标准差回归方程为Y=a+b/X形式,决定系数均在0.9以上. RAPD遗传信息分析精度控制在%～10%时的最适样本容量为1～32条随机引物或120～230个多态性位点.
文自翔, 金文林, 濮绍京, 赵波, 卢为国, 李海朝, 李金英
. RAPD多态位点数变化对小豆遗传变异信息估计的影响[J]. 华北农学报, 2010
, 25(1)
: 75
-79
.
DOI: 10.7668/hbnxb.2010.01.014
RAPD (Random amplified polymorphic DNA) variation was assessed in 163 accessions of adzuki bean from China,Japan,Korea,Bhutan,Nepal and Vietnam.A "population" was constructed by 261 polymorphic loci detected by 27 RAPD primers.The method of entirely random sampling was adopted to analyze the regression relationship between the sizes of sample (allelic number) and genetic similarity index (Jaccard index).Regression equation for different number of primers,loci and Jaccard index was calculated out,and then the reasonable number of primers and loci under given precision can be decided by analyzing the tangent of the curve equation.The results were as follows:There exists significant difference among the S,CV and average of Jaccard index calculated from different number of primers. Regression equation for different number of primers,loci and the CV of Jaccard index was Y=a+b/X,and the determine index was above 0.95.Under the precision of 5%-10%,the reasonable sample should be 15-32 RAPD primers or 120-230 polymorphic loci.
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