水稻苗期冷害是导致水稻减产的重要因素之一.为了发掘新的耐冷基因、诠释其耐冷分子机制并应用分子育种的手段提高水稻苗期耐冷性,前期已通过Northern表达分析,从已构建的水稻苗期低温诱导表达正向抑制差减cDNA文库中,发现了一个受低温诱导上调表达的功能未知的C_2H_2型锌指结构域蛋白新基因(特命名为OsCOI).本研究进一步通过RT-PCR的方法从水稻中克隆了该基因,构建了其植物过表达载体,并通过农杆菌介导转入水稻基因组中,获得了其过表达转基因水稻株系,为进一步研究该基因的功能并探讨其在水稻耐冷分子育种中的应用价值奠定了基础.
Chilling injure in seedling period is one of major limiting factors for productivity of rice(Oryza sativa L.).To improve rice cold tolerance in seedling period,it is important to exploit novel cold-resistant genes,unravel the underlying molecular mechanism and utilize the genes in cold-resistant molecular breeding.A novel cold-inducible gene encoding a putative C2H2-type zinc finger protein,designated OsCOI(Oryza sativa Cold-Inducible),have been identified in our former research through northern blot analysis.In this study,the gene was cloned to a plant binary over-expression vector AHLG and transformed into rice by Agrobacterium-mediated approach.Finally,through PCR screening method,some over-expression transgenic rice lines were acquired.These works established a basis for further studying the function and application potential of the gene in rice seedling cold tolerance.
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