论文

棉花GhRDL1基因启动子分析

  • 上官小霞 ,
  • 吴霞 ,
  • 李燕娥
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  • 山西省农业科学院,棉花研究所, 山西运城 044000
上官小霞(1974-),女,山西阳城人,助理研究员,博士,主要从事棉花外源基因的遗传转化及棉纤维功能基因组研究工作.

收稿日期: 2009-12-09

  网络出版日期: 2014-10-14

基金资助

转基因专项:安全转基因技术(2008ZX08010-3);山西省青年基金项目(2008021038-1)

Analysis of the Promoter of Cotton GhRDL1 Gene

  • SHANGGUAN Xiao-xia ,
  • WU Xia ,
  • LI Yan-e
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  • Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China

Received date: 2009-12-09

  Online published: 2014-10-14

摘要

通过PCR法扩增出陆地棉GhRDL1基因ATG上游1.2 kb的片段,将该片段融合GUS报告基因进行棉花、拟南芥及烟草转化.分析显示,GhRDL1基因启动子是一个棉纤维或表皮毛特异的启动子,能够驱动靶基因在单细胞的表皮毛(如棉纤维和拟南芥表皮毛)表达;在烟草表皮毛中,GUS基因在多细胞的腺毛(包括顶端的腺体细胞和柄细胞)和非腺毛中皆有表达.表明GhRDL1基因启动子是一个多功能的表皮毛特异启动,可以用于棉花基因工程改良和植物次生代谢工程的研究.

本文引用格式

上官小霞 , 吴霞 , 李燕娥 . 棉花GhRDL1基因启动子分析[J]. 华北农学报, 2010 , 25(1) : 6 -10 . DOI: 10.7668/hbnxb.2010.01.002

Abstract

A 1.2 kb upstream fragment of GhRDL1 was isolated form Gossypium hirsutum and fused to a β-glucuronidase(GUS) reporter gene,and was transferred into cotton,Arabidopsis and tobacco.In transgenic cotton plants,GhRDL1 promoter exhibited activities predominantly in developing fiber cellsand 0-DPA ovule and to a less extent in trichomes of other aerial organs,including leaf,stem,bract,sepal and petal.In transgenic Arabidopsis plants the promoter was also specific to trichomes.Different from Arabidopsis and cotton fiber that have unicellular simple trichomes,tobacco has glandular secreting trichomes(GSTs) and simple trichomes,both are multicellular.In tobacco the GhRDL1 promoter directed GUS gene expression exclusively in the GSTs(Including the head cells and stalk cell) and multicellular simple trichomes.This promoter provides a tool not only for genetic engineering of cotton fiber improvement,but also for the GSTs-based plant secondary metabolism engineering.

参考文献

[1] 李杰,张福诚,王文泉,等.高等植物启动子的研究进展[J].生物技术通讯,2006,17(4):658-661.
[2] 朱亚兰,姚伟,窦建华,等.种子特异表达启动子的克隆分析及其植物表达载体的构建[J].华北农学报,2007,22(2):6-10.
[3] John M E,Keller G.Metabolic pathway engineering in cotton:biosynthesis of polyhydroxybutyrate in fiber cell[J].Proceeding of the National Academy of Sciences,USA,1996,93:12678-1277.
[4] HsuC Y,Creech R G,Jenkins J N,et al.Analysis of promoter activity of cotton lipid transfer protein geneLTP6in transgenic tobacco plants[J].Plant Science,1999,143:63-70.
[5] Liu H C,Creech R G,Jenkins J N,et al.Cloning and promoter analysis of the cotton lipid transfer protein gene Ltp3[J].Biochimicaet Biophysica Acta,2000,1487:106-111.
[6] Delaney S K,Orford S J,Martin-Harris M,et al.The fiber specificity of the cotton FSltp4 gene promoter is regulated by an AT-Rich promoter region and the AT-hook transcription factor GhAT1[J].Plant and Cell Physiology,2007,48:1426-1437.
[7] Wang S,Wang J W,Yu N,et al.Control of plant trichome development by a cotton fiber MYB gene[J].Plant Cell,2004,16:2323-2334.
[8] Shangguan X X,Xu B,Yu Z X,et al.Promoter of a cotton fibre MYB gene functional in trichomes of Arabidopsis and glandular trichomes of tobacco[J].Journal of Expreimental Botany,2008,59(13):3533-3542.
[9] 吴霞,焦改丽,吴家和,等.棉花农杆菌介导法转化机理及研究进展[J].山西农业科学,2001,29(1):27-30.
[10] 王彦霞,王省芬,马峙英.棉花转基因技术的研究及应用[J].华北农学报,2006,21(增刊):41-45.
[11] Clough S J,Bent A F.Floral dip:a simplified method for Agrobacterium mediated transformation of Arabidopsis thaliana[J].Plant Journal,1998,16:735-743.
[12] Jefferson R A,Kavanagh T A,Bevan M W.GUS fusions:beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants[J].EMBO Journal,1987,6:3901-3907.
[13] Wang E,Gan S,Wagner G J.Isolation and characterization of the CYP71D16 trichome-specific promoter from Nicotiana tabacum L[J].Journal of Experimental Botany,2002,53:1891-1897.
[14] AbeM,Takahashi T,Komeda Y.Identification of a cis regulatory element for L1 layer-specific gene expression,which is targeted by an L1-specific homeodomain protein[J].Plant Journal,2001,26:487-494.
[15] Plesch G,Ehrhardt T,Mueller-Roeber B.Involvement of TAAAG elements suggests a role for Dof transcription factors in guard cell-specific gene expression[J].Plant Journal,2001,28:455-464.
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