为了研究家兔ATG16L1基因对非特异性消化道紊乱的易感性,试验采用PCR-HRM技术,首次对家兔ATG16L1基因进行单核苷酸多态性(SNP)检测,同时结合低纤维群体结肠ATG16L1基因mRNA表达量进行关联分析。结果表明:ATG16L1基因存在1个SNP位点(c.1482 G>A)与NSDD的易感性相关联;c.1482 G>A位点A等位基因能增加NSDD的易感性(OR=1.43,95%置信区间:1.08～1.91,P<0.05);在隐性遗传模型中,AA基因型能增加NSDD的易感性(OR=1.69,95%置信区间:1.05～2.78,P<0.05);ATG16L1基因在结肠中的mRNA表达量随着炎症的加剧显著升高(P<0.05);AA基因型在整个低纤维诱导NSDD组中的表达水平最低(P<0.05)。这些结果均表明,ATG16L1基因与家兔NSDD的遗传易感性相关。
To study the susceptibility of ATG16L1 gene to non- specific digestive disorder (NSDD) in rabbit， single nucleotide polymorphism (SNP) of rabbit ATG16L1 gene was firstly detected by PCR- HRM and the associa- tion analysis of ATG16L1 gene mRNA expression in colon of fibre- deficient diet group was conducted．The results found that there was a SNP locus (c． 1482 G ＞ A) in ATG16L1 gene and the SNP locus was associated with the sus- ceptibility to NSDD; the allele A of c． 1482 G ＞ A could increase the susceptibility (OR = 1． 43， 95% confidence interval: 1． 08－ 1． 91， P ＜ 0． 05); in recessive genetic model，the genotype AA could increase the susceptibility (OR = 1． 69，95% confidence interval: 1．05－ 2．78，P ＜ 0．05); along with the increasing severity of non- specific digestive disorder， the ATG16L1 gene mRNA expression in colon was gradually increased (P ＜ 0．05); genotype AA had the lowest mRNA expression in fibre- deficient diet group (P ＜ 0．05) ．These results showed that ATG16L1 gene was associated with the genetic susceptibility to NSDD in rabbit．
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