采用水培的方法,以芥菜为材料,研究了施加不同质量浓度外源硒(Se)(1,2,6,8 mg/L)对单一质量浓度镉(Cd)(10 mg/L)胁迫下芥菜种子萌发生理效应的影响。结果表明,单一质量浓度Cd胁迫显著抑制了芥菜种子的发芽(P<0.01),种子萌发过程中幼苗中的可溶性糖含量和淀粉含量显著低于对照(P<0.01),丙二醛(MDA)含量则明显高于对照(P<0.01),表明单一Cd对芥菜种子的萌发产生了一定程度的伤害;单一Cd胁迫下加入不同质量浓度外源Se后,能够有效地提高种子的发芽率,使可溶性糖和淀粉含量增加,MDA含量明显降低,表明外源Se可以有效地缓解Cd对芥菜种子萌发造成的伤害;当外源Se质量浓度为2 mg/L时,芥菜的发芽率、可溶性糖含量和淀粉含量为最大值,且与对照相比变化不大(P>0.05),但均显著高于单一Cd胁迫下的相应值(P<0.01),MDA含量最低,且显著低于对照和单一Cd胁迫下的相应值(P<0.01),表明当外源Se的质量浓度为2 mg/L时,对单一质量浓度Cd胁迫下芥菜种子萌发的缓解作用最佳。
The effects of different concentration exogenous Se(1，2，6，8 mg /L) on physiological effects of ger- mination of mustard seeds under the stress of single concentration Cd(10 mg /L) were studied by hydroponic experi- ment．The results showed that the germination were significantly inhibited(P ＜ 0．01)．The starch content and solu- ble sugar content of seedling during the seed germination was markedly lower than the control group(P ＜0．01)，and the MDA content of seedling was significant higher than those in control(P ＜0．01) under the stress of Cd，which indi- cated that Cd had damage effects on germination of mustard seeds．The germination rate，the starch content and soluble sugar content was increased，and the MDA content was decreased by adding different concentration Se under Cd stress (P ＜0． 05)．It could be concluded that the exogenous Se could effectively alleviate the damage effect by the stress of Cd for the germination of mustard seeds．When exogenous Se concentration was 2 mg /L，the germination rate，the starch content and soluble sugar content were highest，and there were no obviously change compared with the control (P ＞0． 05)，but the all values were significant higher than those of the stress under single concentration Cd(P ＜ 0. 01); the MDA content was lowest and was significant lower than those of the control and the stress under single con- centration Cd(P ＜0． 01)．It could be concluded that when exogenous Se concentration was 2 mg /L，there was best al- leviate effect on the germination of mustard seeds under the stress of single concentration Cd．
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