以有毛亲本F80为母本,无毛突变体F80WM为父本杂交,配制F1、F2、BC1群体,通过对6世代群体有无刚毛特征的观察和统计分析,研究有刚毛性状的遗传规律,并利用BSA法、SSR技术筛选与刚毛性状基因紧密连锁的分子标记。结果表明,黄瓜无毛性状是由1对核基因控制的稳定遗传的隐性性状,有刚毛相对于无毛为完全显性。通过BSA法和SSR分子标记,应用1 193对引物组合对无毛、有毛亲本进行筛选,筛选到了1个与黄瓜有毛性状相关的显性标记SSR01647。测序结果表明,片段长度为164 bp,在有毛个体中扩增出了164 bp的特异片段,具有13个TC重复序列,无毛个体中未扩增出条带。经组外其他F2单株验证发现鉴定结果符合率高达100%。该性状可以作为苗期遗传标记性状,在杂交育种和品种纯度鉴定上有极大的利用价值。
关键词：
黄瓜; 刚毛; 无毛; 分子标记; SSR
The glabrous mutant F80WM was found in the inbred line F80 and can be inherited stably．F2and BC1 population derived from F1 of F80 (trichomes female) and F80WM(glabrous male) was used as materials to study the inheritance and molecular marker associated to the trichomes and glabrous trait．The results indicated that the glabrous trait was controlled by a pair of recessive gene，and the character of trichomes was dominant to that of glabrous．The polymorphism between trichomes and glabrous parents of cucumber were studied using BSA method and SSR technology．1 193 SSR markers were screened，and a dominant marker SSR01647 was obtained． Sequen- cing of the fragment indicated that the lengths were 164 bp．The trichomes F2 plants possessed the 164 bp frag- ments，and the glabrous F2 plants were no fragments．The marker was closely linked to the cucumber trichomes trait- related gene，and the marker was confirmed to be 100% accurate by F2 individuals．The trait was a useful marker for hybridization and purity identification of F1 hybrids．
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