论文

中国荷斯坦牛BoLA-DRB3基因的mRNA表达研究

  • 王学清 ,
  • 李魁英 ,
  • 裴翠娟 ,
  • 张峰 ,
  • 吴占军 ,
  • 刘小虎 ,
  • 马书林 ,
  • 张新同 ,
  • 王昆
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  • 河北省农林科学院粮油作物研究所, 河北石家庄 050035
王学清(1981- ),男,河北平山人,助理研究员,硕士,主要从事动物遗传育种研究

收稿日期: 2013-04-26

  网络出版日期: 2014-10-14

基金资助

河北省自然科学基金项目(C2012301011);河北省农林科学院财政项目(2010055008)

Expression of BoLA- DRB3 mRNA in Chinese Holstein Cows

  • WANG Xue-qing ,
  • LI Kui-ying ,
  • PEI Cui-juan ,
  • ZHANG Feng ,
  • WU Zhan-jun ,
  • LIU Xiao-hu ,
  • MA Shu-lin ,
  • ZHANG Xin-tong ,
  • WANG Kun
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  • Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, China

Received date: 2013-04-26

  Online published: 2014-10-14

摘要

为揭示牛MHC-DRB3上游调控区多态对其表达调控作用,利用PCR-SSCP和DNA序列测定技术分析并建立BoLA-DRB3*exon2和BoLA-DRB3 URR之间的连锁关系。结合不同近端调控区与转录因子结合模式预测结果,选择3种不同上游调控序列的连锁组合,应用实时定量RT-PCR技术对牛MHC-DRB3基因mRNA相对表达量进行了验证分析。结果表明,上游调控区基因型为H17H17的BoLA-DRB3基因mRNA相对表达量显著高于(P<0.01)基因型H1H1及H5H6。初步证明上游调控区中的多态会引起结构基因表达量的变化。

本文引用格式

王学清 , 李魁英 , 裴翠娟 , 张峰 , 吴占军 , 刘小虎 , 马书林 , 张新同 , 王昆 . 中国荷斯坦牛BoLA-DRB3基因的mRNA表达研究[J]. 华北农学报, 2013 , 28(4) : 19 -22 . DOI: 10.3969/j.issn.1000-7091.2013.04.004

Abstract

To study the regulation of upstream regulatory region polymorphisms on BoLA- DRB3 gene expres- sion,PCR- SSCP and DNA sequencing were used to identify the linkage relationship between BoLA- DRB3* exon2 and BoLA- DRB3 URR.Combined with the predicted results of binding mode between different URR and transcrip- tion factor,three linkage types were selected to detect the BoLA-DRB3 mRNA levels by relative Real- time PCR ex- periments. The results showed that the expression abundances of BoLA-DRB3 varied in different linkage types.The expression level of mRNA of BoLA- DRB3 gene linked with H17H17 was significant higher than H1H1 and H5H6 (P<0.01).This preliminary proved that the polymorphism of upstream regulatory region can cause the expression change of BoLA- DRB3 structural gene.

参考文献

[1] Klein J. Origin of MHC Polymorphisms: the trans-species hypothesis[J]. Human Immunology,1987,19: 155-62.
[2] Babbit B P,Allen P M. The binding of immunogenetic peptides to Ia histocompatibility molecules[J]. Nature,1987,317: 359-362.
[3] Buus S,Sette A. The relation between major histocompati-bility complex (MHC) restriction and the capacity of Ia to bind immunogenetic peptides[J]. Science,1987,235: 1353-1355.
[4] Schmittgen T D,Zakrajsek B A. Effect of experimental treatment on housekeeping gene expression: validation by real-time,quantitative RT-PCR[J]. J Biochem Biophys Methods,2000,46(1-2): 69-81.
[5] Russell G C. BoLA class nucleotide sequences,1996: report of the ISAG BoLA Nomenclature Committee[J]. Animal Genetics,1997,28: 169-180.
[6] Anim Gelhaus,Sequence and PCR-RFLP analysis of 14 novel BoLA-DRB3 alleles[J]. Animal Genetics,1995,26: 147-153.
[7] Wang Kun,Sun Dong xiao,Zhang Yuan. Sequencing of 15 new BoLA-DRB3 alleles[J]. International J Immunogenet-ics,2008: 331-332.
[8] 王学清,王 昆,王沛宇,等. 中国荷斯坦牛MHC-DRB3 * exon2 的 PCR-SSCP 多态性分析[J]. 华北农学报,2010,25(5): 94-98.
[9] 王 昆,孙东晓,徐如海,等. BoLA-DQB 上游调控区多 态性研究[J]. 畜牧兽医学报,2005,36(6): 521-525.
[10] Wang K,Sun D X,Li K Y,et al. Identification of four novel alleles of the BoLA-DRB3 upstream regulatory re-gion in Chinese yellow cattle[J]. Tissue Antigens,2012,80: 58-60.
[11] Ripoli M V,Peral-Garcia P,Dulout F N,et al. Polymor-phism in the bovine BoLA-DRB3 upstream regulatory re-gions detected through PCR-SSCP and DNA sequencing [J]. Gene,2004,339: 71-78.
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