在经过高代自交选育出结球甘蓝高抗霜霉病自交系R103和感霜霉病自交系S101的基础上,根据苗床田间自然发病的S101病害症状,以及病原物镜检和回接鉴定,明确该病害的病原是十字花科霜霉菌。经过苗期田间自然诱发和十字期人工室内接种抗性鉴定,以感病亲本为回交亲本的BC1群体中抗病株和感病株的比例,田间是0.91∶1,室内为0.96∶1,经卡平方适合性检测均符合1∶1分离比例;F2分离群体中自然鉴定和人工鉴定抗病单株和感病单株的比例分别为2.93∶1和3.01∶1,检测结果经卡平方分析证明均符合3∶1的分离比例,2种抗性鉴定方法都表明这些群体中结球甘蓝霜霉病抗性受单基因显性控制,为了缩短抗性鉴定时间,可采用人工接种鉴定代表整个苗期结球甘蓝霜霉病抗性。
We confirmed that the pathogen of cabbage downy mildew was obligate cruciferae Hyaloperonospora parasitica,according to the symptom of the susceptive S101 in filed,and the result of pathogen microscopical examination and re-inoculating on susceptive materials having no disease. Then did the genetic analysis by natural induction in field and artificial inoculation in greenhouse, the proportions of resistant plants and susceptive plants were 0. 91 ∶ 1( in field) and 0.96∶ 1( in greenhouse) in the backcross population constructed by F1 crossing susceptive parent S101,as are in accordance with 1∶ 1 separation rate assayed by the Chi-square test. In the F2 separation population,the proportions were 2. 93∶ 1( in field) and 3.01 ∶1( in greenhouse) ,being in accordance with 3 ∶ 1 separation rate by the same test. Two kinds of resistance identification methods show that the resistance to downy mildew of these population is monogenic inheritance, so we could use the artificial inoculation in cross stage to identify the resistance to cabbage downy mildew in entire seedling stage simply and fastly.
[1] Constantinescu O,Fatehi J. Peronospora-like fungi( Chromista,Peronosporales) parasitic on Brassicaceae and related hosts[J]. Nova Hedwigia, 2002, 74: 291 - 338.
[2] Persoon C H. Observationes Mycologicae[M]. Wolf,Leipzig,1796: 1.
[3] 王铨茂. 十字花科霜霉病菌生理分化及寄主抗病现象[J]. 科学农业, 1944,1: 249 - 257.
[4] Kucharek T. Downy mildew of Crucifers[J]. Plant Pathology Fact Sheet, 2000: 33.
[5] Felton M W,Walker J C. Environmental factors affecting downy mildew of cabbage[J]. J Agric Res, 1946, 72: 69 -81.
[6] Koch E ,Slusarenko A J. Arabidopsis is susceptible to infection by a downy mildew fungus[J]. Plant Cell,1990,2: 437 - 445.
[7] Mauch-Mani B,Slusarenko A J. Downy mildew of Arabidopsis thaliana[M]/ /Bowman J L. Arabidopsis: An Atlas of Morphology and Development. New York: Springer,1994: 414 - 417.
[8] 李寿田,汪隆植,龚义勤,等. 萝卜霜霉病抗性鉴定及抗性品种筛选[J]. 中国蔬菜, 2002,1: 30 - 31.
[9] 王超,吴世昌,秦智伟,等. 甘蓝苗期多抗性鉴定技术研究[J]. 东北农业大学学报, 2000, 31( 2) : 152 - 59.
[10] Coelho P S,Valério L,Monteiro A A. Leaf position, leaf age and plant age affect the expression of downy mildew resistance in Brassica oleracea[J]. Eur J Plant Pathol, 2009, 125: 179 - 188.
[11] Natti J J,Dickson M H,Atkin J D. Resistance of Brassica oleracea varieties to downy mildew[J]. Phytopathology,1967, 57: 144 - 147.
[12] Jensen B D,bak S V,Munk L, et al. Characterization and inheritance of partial resistance to downy mildew,Peronospora parasitica, in breeding material of broccoli,Brassica oleracea convar. Botrytis var. italica [J]. Plant Breeding, 1999, 118( 6) : 549 - 554.
[13] Farinhó M,Coelho P,Monteiro A, et al. SCAR and CAPS markers flanking the Brassica oleracea L. Pp523 downy mildew resistance locus demarcate a genomic region syntenic to the top arm end of Arabidopsis thaliana L. chromosome 1[J]. Euphytica, 2007, 157: 215 - 221.
[14] Malin C,Roland V B,Arnulf M. Screening and evaluation of resistance to downy mildew( Peronospora parasitica) and clubroot ( Plasmodiophora brassicae) in genetic resources of Brassica oleracea[J]. Hereditas,2004,141( 3) : 293 - 300.
[15] Hoser-Krauze J,Lakowska-Ryk E,Antosik J. The inheritance to resistance to some Brassica oleracea L. cultivars and lines to downy mildew-Peronospora parasitaca ( Pers. ) ex Fr[J]. J Appl Genet, 1995, 36: 27 - 33.
[16] Monteiro A A,Coelho P S,Bahcevandziev K, et al. Inheritance of downy mildew resistance an cotyledon and adultplant stages in‘Couve Algarvia’( Brassica oleracea var. tronchuda) [J]. Euphytica, 2005, 141( 1 - 2) : 85 -92.
[17] 程永安,柯桂兰. 影响大白菜霜霉病抗性鉴定的因素[J]. 西北农业学报, 1995,4( 4) : 69 - 72.
[18] Jensen B D,Hockenhull J,Munk L. Seeding and adult plant resistance to downy mildew( Peronospora parasitica) in cauliflower ( Brassica oleracea convar. Botry var.botrytis) [J]. Plant Pathol, 1999, 48: 604 - 612.
[19] 李树德,方中远,李明远,等. 中国主要蔬菜抗病育种 进展[M]. 第1 版. 北京: 北京科学出饭社, 1995: 60 -67.
[20] Yerkes W D,Shaw C G. Taxonomy of the Peronospora species of Cruciferae and Chenopodiaceae[J]. Phytopathology,1959, 49: 499 - 507.
[21] 邢来君,李明春. 普通真菌学[M]. 第11 版. 北京: 高等教育出版社, 1999: 301 - 303.
[22] Coelho P S,Bahcevandziev K,Valério L, et al. The relationship between cotyledon and adult plant resistance to downy mildew( Peronospora parasitica) in Brassica Oleracea[J]. Acta Hort( ISHS) , 1998, 459: 335 - 342.
[23] 李怀方,刘分权,郭小密. Horticultural Pathology[M].北京: 中国农业大学出版社, 2001: 127 - 130.
[24] Yu ShC,Zhang F L,Zhao X Y, et al. Sequence-characterized amplified region and simple sequence repeat markers for identifying the major quantitative trait locus responsible for seedling resistance to downy mildew in Chinese cabbage ( Brassica rapa ssp. pekinensis) [J]. Plant Breeding, 2011, 130: 580 - 583.
[25] Farnham M W,Wang M,Thomas C E. A single dominant gene for downy mildew resistance in broccoli[J]. Euphytica,2002, 128: 405 - 407.
[26] Coelho P S,Monteiro A A. Inheritance of downy mildew resistance in mature broccoli plants [J]. Euphytica,2003, 131( 1) : 65 - 69.