为防治土传病害,克服黄瓜连作障碍,探讨了土壤生物添加剂不同添加量对土壤微生物种群结构的影响.结果表明,土壤添加剂施用后不同时期,土壤微生物总量增多,土壤细菌和放线菌变化较大,真菌变化不大.优势菌的种类和数量发生变化,木霉、青霉等一些有益菌数量明显增加,根际优势种的抑菌作用较强.施用添加剂后,提高了土壤微生物多样性,能够调节土壤微生物群落结构,比应用化学农药更具有生态安全性.
Soil biologic amendment (BSA)was applied to the cucumber continuous cropping to control soil2borne dis2 eases and reduce replant diseases of cucumber. The changes of soil microorganism population structure were discussed by different agent. The result showed that total population of cucumber rhizosphere microbes increased remarkably after ap2 plying soil amendment.Number of bacteria and actinomycetes had a drastic variation.Number of fungi increased slightly. The species and amount of the dominant species were changing,beneficial microbes such as Trichoderma and Penicillium were more.Dominant species in rhizosphere antagonism were stronger along with controlling effect increasing. Applying BSA could increase soil microbial diversity and adjust community structure of soil microbial,and had better ecological sta2 bility than fungicide.
[1] 吴凤芝,王伟,栗非时.土壤灭菌对大棚连作黄瓜生长发育影响[J].北方园艺999(5):49.
[2] 袁飞,彭宇,张春兰,等.有机物料减轻设施连作黄瓜苗期病害的微生物效应[J].应用生态学报,20045(5):867-870.
[3] 吕卫光,张春兰,彭宇,等.外源苯丙烯酸抑制连作黄瓜生长的机制初探[J].中国蔬菜,2001(3):10-12.
[4] 方中达.植病研究方法[M].北京:中国农业出版社998.
[5] 王振,赵廷昌,刘学敏,等.转基因作物对土壤微生物多样性影响[J].植物保护,2007,33(4):15-19.
[6] 廖宗文.工业废物的农用资源化:理论、技术和实践[M].北京:中国环境科学出版社996:169-174.
[7] 蔡燕飞,廖宗文,董春,等.番茄青枯病的土壤微生态防治研究[J].农业环境保护,2002,21(5):417-420.