试验旨在观察赛啦唑对大鼠不同脑区突触体Ca2+-ATP酶活性的影响,以探讨其在全麻分子机理中的作用.Wistar大白鼠8只,随机取12只为生理盐水对照组,其余随机均分为低剂量赛啦唑用药组(ip赛啦唑80 mg/kg)和高剂量赛啦唑用药组(ip赛啦唑100 mg/kg),每个剂量组又分为麻醉期、翻正反射恢复期和苏醒期三个亚组(各12只).采用差速离心法分离了大鼠大脑皮质、小脑、海马和脑干突触体,比色法测定了不同脑区突触体Ca2+-ATP酶活性.结果表明:赛啦唑明显地抑制大鼠大脑皮质、小脑、海马和脑干突触体Ca2+-ATP酶的活性,并且其抑制作用呈现出明显的剂量依赖性增强趋势,这种变化趋势与大鼠ip赛啦唑麻醉后行为学变化基本一致.结果提示:大脑皮质、小脑、海马和脑干突触体Ca2+-ATP酶是赛啦唑全麻作用的靶位之一.
:This experiment was aimed to determine the influence of xylazole on Ca2 +-ATPase activities in rats′different brain regions in order to clarify its general anesthetic mechanism.84 Wistar rats were randomly divided into conrol group(12 rats), high dose xylazole group (ip.xylazole 100mg/kg)and low dose xylazole group (ip.xylazole 80 mg/kg), and each dose group was divided into three sub-groups, eg anesthesia group, recovery of righting reflex and aroused group. Rats'brain samples of cerebral cortex, hippocampus, cerebellum and brain stem in different period under xylazole anesthesia were separated by differential centrifugation.The Ca2+-ATPase activities of synaptosome in above brain brain regions were determined by using colorimetry methods.The results showed that, xylazole inhibited Ca 2+-ATPase activities of synaptosomal membranes in cerebral cortex, hippocampus, cerebellum and brain stem in a dose-dependent manners.The inhibition on Ca2+-ATPase activities of synaptosome in these brain regions fitted with the behavioral changes in rats after being administrated with xylazole intraperitoneally.The results indicated that Ca2 +-ATPase in synaptosome in cerebral cortex, hippocampus, cerebellum and brain stem might be the targets of general anesthetic action mediated by xylazole.
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