论文

玉米自交系AS-9化学诱变后代SSR遗传变异分析

  • 李红英 ,
  • 卢存福 ,
  • 兰小中 ,
  • 杨凤娇 ,
  • 金德善 ,
  • 乔佩 ,
  • 卢骁 ,
  • 玉猛 ,
  • 陈玉珍
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  • 1. 北京林业大学生物科学与技术学院林木育种国家工程实验室, 北京 100083;
    2. 西藏农牧学院, 西藏林芝 860000
李红英(1985-),女,河北张家口人,在读硕士,主要从事植物分子生物学方面的研究

收稿日期: 2013-03-29

  修回日期: 2013-03-29

  网络出版日期: 2014-10-14

基金资助

国家科技支撑计划项目(2011BAI13B06);北京市自然科学基金(6112016);国家自然科学基金(31270737)

Genetic Variation Analysis in Maize Mutants from AS-9 Inbread Line Based on SSR Marker

  • Li Hongying ,
  • Lu Cunfu ,
  • Lan Xiaozhong ,
  • Yang Fengjiao ,
  • Jin Deshan ,
  • Qiao Pei ,
  • Lu Xiao ,
  • Yü Meng ,
  • Chen Yuzhen
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  • 1. College of Biological Sciences and Biotechnology,National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants Supported Ministry of Education,Beijing Forestry University, Beijing 100083,China;
    2. Tibet Agricultural and Animal Husbandry College, Linzhi 860000,China

Received date: 2013-03-29

  Revised date: 2013-03-29

  Online published: 2014-10-14

摘要

为分析系列化学诱变对玉米自交系AS-9诱变后代的影响,以玉米自交系AS-9及其化学诱变自交第1代M1和第4代M4为材料,进行发芽试验,同时利用分布在10条染色体上的68对SSR引物进行遗传变异分析。结果表明:诱变M1和M4种子的发芽率、芽长和根数普遍高于对照;能在2代玉米试材中扩增出多态性条带的引物有54对,占引物总数的79.41%;M1诱变系与基础材料对照的遗传相似系数平均值为0.364 7,遗传相似系数中心化后的数据在-0.12处,可将材料分为2个类群,基础材料(ⅠCK)为一类,诱变系为一类;M4诱变系与基础材料对照的遗传相似系数平均值为0.434 6,遗传相似系数中心化后的数据在-0.09处,可将材料分为基础自交系(ⅣCK)和诱变系2个类群;M1和M4主成分分析与聚类结果基本一致。说明基础材料AS-9与诱变系材料间存在真实的遗传差异,系列化学诱变已使玉米自交系AS-9产生了广泛的遗传变异。

本文引用格式

李红英 , 卢存福 , 兰小中 , 杨凤娇 , 金德善 , 乔佩 , 卢骁 , 玉猛 , 陈玉珍 . 玉米自交系AS-9化学诱变后代SSR遗传变异分析[J]. 华北农学报, 2013 , 28(3) : 92 -101 . DOI: 10.3969/j.issn.1000-7091.2013.03.018

Abstract

Analysis of genetic diversity in maize germplasm is of great importance to conserving and utilizing these valuable chemical mutagenic resources. In this research, the genetic differences between mutants ( M1&M4) and the corresponding orginal material( AS-9) was studied based on SSR markers with selected 54 polymorphism SSR primers. The results showed that the seed germination rate,bud length and root number of M1 &M4 were higher than AS-9 in most cases. In two generations maize materials,54 polymorphism primers can be amplified,accounting for 79. 41% of the total. Relatived toⅠCK , the average value of genetic similarity coefficient of M1 was 0.364 7.With the certered genetic similarity coefficient of - 0.12 by UPGMA, the maize inbred line materials were divided into two groups: the orginal material(ⅠCK) and mutants. Relatived to ⅣCK, the average value of genetic similarity coefficient of M4 was 0.434 6. With the cemtered genetic similarity coefficient of - 0.09, the maize materials were classified into two groups: ⅣCK and mutants. Principal component analysis and clustering results were similar. The results showed there were distinct variation between orginal material AS-9 and induced material, indicating chemical mutagen had produced genetic variations in maize inbred lines on large scale.

参考文献

[1] 徐明,路铁刚. 植物诱变技术的研究进展[J]. 生物技术进展, 2011,1( 2) : 90 - 97.
[2] 刘录祥,郭会君,赵林姝,等. 我国作物育种20 年的基本成就与展望[J]. 核农学报, 2007, 21( 6) : 589 - 592.
[3] 李奇,石海春,柯永培,等. 玉米自交系48 - 2 和R08辐照后代M3 株系遗传变异的SSR 分析[J]. 核农学报, 2011, 25( 6) : 1100 - 1106.
[4] 覃鸿妮,蔡一林,杨春蓉,等. 玉米诱变系的SSR 遗传变异分析[J]. 核农学报, 2008, 22( 6) : 750 - 755.
[5] 秦家友,石海春,柯永培,等. 玉米辐射诱变系表型及SSR 遗传差异研究[J]. 玉米科学,2012,20( 2) : 41 -47.
[6] 魏良明,姜鸿勋,胡学安,等. 植物诱变新技术及其在玉米育种上的应用[J]. 玉米科学,2000,8 ( 1) : 19 -20.
[7] 赵霞,周波,李玉花,等. T-DNA 插入突变在植物功能基因组学中的应用[J]. 生物技术通讯,2009,20( 6) : 317 - 321.
[8] Walden R,Hayashi H,Schell J. T-DNA as a gene tag[J]. Plant, 1991,1( 3) : 281 - 288.
[9] Hiei Y,Ohta S,Komari T, et al . Efficient transformation of rice ( Oryzasativa L. ) mediated by agrobacterium and sequence analysis of the boundaries of the T-DNA[J].Plant Journal, 1994,6( 2) : 271 - 282.
[10] 郭龙彪,储成才,钱前. 水稻突变体与功能基因组学[J]. 植物学通报, 2006, 23( 1) : 1 - 13.
[11] Li Y H,Qian Q,Zhou Y, et al. BRITTLE CULM1,which encodes a COBRA-like protein,affects the mechanical properties of rice plants[J]. Plant Cell, 2003, 15: 2020 -2031.
[12] Haga K,Takano M,Neumann R, et al. The rice COLEOPTILE PHOTOTROPISM1 gene encoding an ortholog of Arabidopsis NPH3 is required for phototropism of coleoptiles and lateral translocation of auxin[J]. Plant Cell,2005, 17: 103 - 115.
[13] Inukai Y,Sakamoto T,Ueguchi-Tanaka M, et al. Crown rootless 1,which is essential for crown root formation in 3 期李红英等: 玉米自交系AS-9 化学诱变后代SSR 遗传变异分析101 rice, is a target of an auxin response factor in auxin signaling[J]. Plant Cell, 2005, 17: 1387 - 1396.
[14] 叶亚峰. 水稻脆秆突变体遗传与基因定位研究[D].芜湖: 安徽大学, 2012.
[15] 乔晓,石海春,柯永培,等. 玉米航天诱变SP3 株系的遗传变异分析[J]. 玉米科学,2012,20 ( 3) : 15 -21.
[16] 蔡一林,何晓阳. 玉米单交种和地方品种的辐射效应及亲子相关性研究[J]. 核农学报,1995,9( 2) : 81 -85.
[17] 杨红善,常根柱,包文生. 紫花苜蓿的航天诱变[J].草业科学, 2013, 30( 2) : 253 - 258.
[18] 李清国,付晶,钮力亚,等. 化学诱变及其突变体筛选在育种中的应用[J]. 河北农业科学, 2010, 14( 5) :68 - 72.
[19] 崔霞,梁燕,李翠,等. 化学诱变及其在蔬菜育种中的应用[J]. 西北农林科技大学学报,2013,41( 3) : 1 - 8.
[20] 朱保葛,路子显,耿玉轩,等. 烷化剂EMS 诱发花生性状变异的效果及高产突变系的选育[J]. 中国农业科学, 1997, 30( 6) : 87 - 89.
[21] 张兵. EMS 诱导黄瓜突变体的初步研究[D]. 泰安: 山东农业大学, 2012.
[22] 尹冬冬,安调过,李立会,等. 分子标记技术在黑麦研究中的应用[J]. 中国生态农业学报,2011,19 ( 2) :477 - 483.
[23] Smith J S C,Chin E C L,Shu H , et al. An evaluation of the utility of SSR loci as molecular markers in maize( Zea mays L.) comparison with data from RFLPs and pedigree[J]. Theor Appl Genet, 1997, 95( 1 - 2) : 163 -173.
[24] Ajmone-Marsan P,Castiglioni P,Fusari F, et al. Genetic diversity and its relationship to hybrid performance in maize as revealed by RFLP and AFLP markers[J]. Theor Appl Genet, 1998, 96( 2) : 219 - 227.
[25] Reif J C,Melchinger A E,Xia X C, et al. Genetic distance based on simple sequence repeats and heterosis in tropical maize population[J]. Crop Sci,2003,43 ( 4) :1275 - 1282.
[26] Wang Feng-ge,Tian Hong-li,Zhao Jiu-ran, et al. Development and characterization of a core set of SSR markers for fingerprinting analysis of Chinese maize varieties[J]. Maydica, 2011, 56( 1) : 1686 - 1693.
[27] Yu Y,Wang R,Shi Y, et al. Genetic diversity and structure of the core collection for maize inbred lines in China[J]. Maydica, 2007, 52: 181 - 194.
[28] Wang Feng-ge,Zhao Jiu-ran,Dai Jing-rui, et al. Selection and development of representative simple sequence repeat primers and multiplex SSR sets for high throughput automated genotyping in maize [J]. Chinese Science Bulletin, 2007, 52(2) : 215 - 223.
[29] 刘志斋,吴迅,刘海利,等. 基于40 个核心SSR 标记揭示的820 份中国玉米重要自交系的遗传多样性与群体结构[J]. 中国农业科学, 2012, 45( 11) : 2107 - 2138.
[30] 李晓辉,李新海,李文华,等. SSR 标记技术在玉米杂交种种子纯度测定中的应用[J]. 作物学报, 2003, 29( 1) : 63 - 68.
[31] Saghai-Maroof M A,Soliman K M, Jorgensen R A,et al.Ribosomal DNA spacer-length polymorphisms in barley:mendelian inheritance,chromosomal location,and population dynamics[J]. PNAS,1984,81 ( 24 ) : 8014 - 8019.
[32] 张微. 利用RIL 群体定位玉米苗期根系QTL 的研究[D]. 北京: 中国农业大学, 2005.
[33] Kostovaa A,Todorovskaa E,Christova N, et al. Assessment of Genetic Variability Induced by Chemical Mutagenesis in Elite Maize Germplasm via SSR Markers[J].Crop Improvement, 2006, 16( 1 - 2) : 37 - 48.
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