研究不同剂量的硫酸铜对小鼠睾丸组织的损伤,为研究动物生殖毒性和繁衍提供参考依据。首先采用上下法测定硫酸铜对小白鼠的半数致死量LD50为55 mg/kg,95%可信限区间为35.02～155。将40只小白鼠随机分为4组,即对照组(生理盐水)、低剂量组、中剂量组、高剂量组,采用灌胃法染毒。期间记录小鼠体质量,染毒结束后采集睾丸组织称质量并制作石蜡切片,观察其病理组织学变化。结果显示,与对照组相比,不同剂量的试验组体质量的增长率均下降,高剂量组在第1周和第2周甚至出现了负增长,分别为:-3.86%,-0.62%。光学显微镜观察睾丸的病理切片,与对照组相比,试验组小鼠睾丸曲精细管呈现不同程度的变性,管内各级生精细胞数目减少或缺失,精子形成极少或无,且随剂量的升高,睾丸组织病理损伤越严重。以上结果表明,硫酸铜可诱导生精细胞凋亡,且可显著抑制精原细胞的增殖,随着剂量的增加对睾丸组织的生殖毒性效应越强。
According to the study on histological damage induced by the different doses of copper sulfate， this paper can provide reference for the study of animal reproductive toxicity and reproduction． Firstly，LD50 was 55 mg /kg ( 95% CI， 35． 02 to 155) which was determined by Up-and-Down Procedure( UDP) ． 40 rats were randomly separated into control group( normal saline) ， low-dose copper sulfate group，middle-dose copper sulfate group，high-dose copper sulfate group． The rats were exposed to copper sulfate by gavage every day and the treatment was lasted for 28 days．Body weight of rats were recorded during experiment， organ coefficient of tissues and pathology changes of testis were observed after poisoning． The results showed that， in comparison with control group，body weight increasing rate was differently decreased on body weight in each poisoning group． The high-dose group experienced negative growth in the first and second weeks， and were -3． 86%，-0． 62% respectively． Obvious pathological changes through light microscope were observed in testis tissues，seminiferous tubules showed degeneration in varying degrees in poisoning groups． The number of all the spermatogenic cells in each grade in seminiferous tubules and spermatozoa were decreased or absent obviously． Testicular pathologic injury was more serious with the increase of concentration． To sum up， copper sulfate can induce the apoptosis of spermatogenic cells， and can significantly inhibit the proliferation of spermatogonia． The effect of reproductive toxic on testicular tissue are stronger as doses increased
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