水稻的芒性是一个与驯化相关的重要性状,是由多基因控制的数量性状,遗传基础复杂,且易受环境因素影响。染色体单片段代换系由于减少了个体间遗传背景的干扰,已经成为鉴定复杂性状QTL的新型遗传材料。以籼稻品种广陆矮4号为受体和粳稻品种日本晴为供体构建而成的119个染色体单片段代换系为材料,对芒长性状进行了调查分析。结合2011,2012年的调查结果,通过代换作图共鉴定出15个与芒性相关的QTL,这些QTL分布在除1,10,12外的9条染色体上,第3染色体上检测到的QTL最多为3个。15个QTL中效应最大的QTL是qAN4-1,2年平均芒长为4.7 cm;效应最小的QTL是qAN11-2,2年平均芒长为0.7 cm。这些QTL的定位,为进一步克隆芒性相关基因以及研究芒性的分子机理提供了重要的依据。
Awn character in rice relating with domestication is a typical quantitative trait which is liable to be influenced by genetic backgrounds and environments. As a novel research material,chromosome single segment substitution lines are useful in QTL identification because of diminishing the interference of genetic backgrounds among plants. In this study 15 QTLs for awn character were identified using 119 rice chromosome single segment substitution lines derived from Nipponbare as donor parent in the background of Guangluai 4 in 2011 and 2012. A total of 15 QTLs for awn character distributed on 9 chromosomes except chromosome 1,10 and 12 by substitution mapping with maximum 3 QTLs on chromosome 3 .Of these 15 QTLs,qAN4-1 had the largest effect with 4.7 em mean awn in the two years ,while qANl l-2 had the smallest effect with 0.7 em mean awn in the two years. These results provided important basis for the QTLs cloning and molecular breeding of rice.
[1] Nagao S,Takahashi M E. Trial construction of twelve linkage groups in Japanese rice(Genetical studies on rice plant,XXVIII) [J] . J Fac; Agr Hokkaido Univ,1963，53:72-130.
[2] Iwata N,Omura T. Linkage analysis by the reciprocal translocation method in rice plants ( Oryza .saliva L)IILinkage groups。orrespondingto the chromosomes 5，6, 8，9，10 and 11 [J] . Sci Bull Fac Agr Kyushu Univ , 1971，25:137-153
[3] 王开锡，张居念稻芒及其在杂交后代表现的调查田福建稻麦科技，2003,21 (1):9-10
[4] Kowata H , Hatakeyama H. Effects oI cool water irrigation treatment on the awn c;harac;ters in rice [J] .Tohoku .lournal of Crop Science,1997 ,40:1-3
[5] Takamure [J] Kinoshita T. Linkage analysis in chromosomes 3 and 6[J] .Rice Genet Newsl,1991，8:98-99
[6] http://www. gramme. org
[7] Xiong L Z,Liu K D, Dai X K , et al. Identification of genetice factors controlling domestication-related traits of rice using an F2= population of a cross between Oryza .saliva and O. rufipogon [J] Theor Appl Genet 1999, 98:243-251
[8] Thomson M,Tai T,Mc;Clung A, et al. Mapping quantitalive trait loci for yield ,yield components and morphological traits in an advanced bac;kcross population between Oryza rufipogo}a and the Oryza .saliva cultivar Jefferson[J] . Theor Appl Genet ,2003，107:479-493
[9] 胡广隆，张冬玲，潘慧乔，等关联分析定位水稻第4号染色体的芒性基因并结合连锁分析精细定位Awn4. 1[J]科学通报，2010,55(28):2777-2781
[10] Paterson A H , Deverna JW，Lanini B , et al. Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes in an interspecies cross of tomato[J]Genetics，1990,124:735-742
[11] Young N D ,Tanksley S D. Restriction fragment length polymorphism maps and the concept of graphical genotypes[J]Theor Appl Genet , 1989,77(1):95-101
[12] WengJF}GuSH，Wan X Y , et al. Isolation and initial characterization of GM5，a major QTL associated withrice grain width and weight [J] . Cell Res, 2008，18(12):1199-1209
[13] Li Y B, Fan C C, Xing Y Z, et al. Natural variation in G SS plays an important role in regulating grain size and yield in rice[J] . Nat Genet, 2011，43(12):1266-1269
[14] Jin J, HuangW，Gan J P, et al. Genetic; control of rice plant architecture under domestication[J].Nat Genet,2008,40,1365-1369
[15] Shomura A, Izawa T, Ebana K, et al. Deletion in a gene associated with grain size increased yields during rice domestication[J].Nat Genet,2008,40,1023-1028
[16] Tan L, Li X, Liu F, et al. Control of a key transition from prostrate to erect growth in rice domestication[J].Nat Genet,2008,40,1360-1364
[17] 林静，朱文银，张亚东，等利用染色体单片段置换系对水稻芒性状的4个QTLs进行定位分析[J].分子植物育种，2010 , 8(3):426-431
[18] 肖坷，巩迎军，张俊芝，等水稻芒长及其分布特征相关QTL的定位[J].上海师范大学学报:自然科学版，2008,37(6):606-612
[19] Matsushita S, Kurakazu T, Sobrizal N，et al. Mapping of genes for awn in rice using Oryza meridionalis introgreslion lines[J].Rice Genet News1,2003,20:17-18
[20] Linh L H , Jin F X , Kang K H ,et al. Mapping quantitative trait loci for heading date and awn length using an advanced backcross line from a cross between Oryza .sativa and O. minu ta [J] . Breeding Sci,2006,56 (4):341-349
