为阐明高渗透压甘油激酶在链格孢菌中的功能,通过遗传学手段,构建了细极链格孢菌(Alternaria tenuissima)cDNA酵母表达文库,并对其进行筛选而获得细极链格孢菌高渗透压甘油蛋白激酶AtHOG1基因,该基因全长1 539 bp,编码355个氨基酸.AtHog1p含有MAPK保守的激酶激活区域"TGY",与来源于烟曲霉(Aspergillus fumigatus)AfOsm1p(XP-7524)、稻瘟菌(M.grisea)MG01822(XP-3389)及酿酒酵母(S.cerevisiae)ScHog1p(AAB7558)高度同源,相似性分别为94%,90%和80%.在高渗环境下,AtHOG1基因与ScHOG1基因功能相同.链格孢菌中存在HOG通路信号途径,AtHOG1基因可能与链格孢菌的逆境适应性调节密切相关.
冯飞
,
纪春艳
,
杨秀芬
,
曾洪梅
,
邱德文
. 细极链格孢菌Hog1 MAPK(酵母)同源基因AtHOG1的克隆与功能分析[J]. 华北农学报, 2009
, 24(4)
: 74
-79
.
DOI: 10.7668/hbnxb.2009.04.015
An Alternaria tenuissima cDNA expression library was constructed. The MAP kinase HOG1 was isolated from the Alternaria tenuissima cDNA expression library,designated as AtHOG1. It had a size of 1 539 bases in length,en2 coded a protein of 355 amino acids. The AtHog1p contained the conserved TGYactivation loop found in the stress2activat2 ed protein kinase subgroup of MAPKs and its amino acid sequence showed 94 %,90 % and 80 % identities with AfOsm1p (XP2752664)of Aspergillus f umigatus,Mg01822 (XP2363896)of Magnaporthe grisea and ScHog1p (AAB67558)of Sac2 charomyces cerevisiae, respectively. AtHOG1 cDNA sequences could complement the functions of S. cerevisiae ScHOG1 genes in sodium chloride tolerance,suggesting a functional HOGpathway exists in A. tenuissima,AtHOG1 gene was in2 volved in the stress adaptation regulation of A. tenuissima.
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