2011年从山东不同地区的发病鸡体内分离到4株新城疫病毒,分别命名为SD1～SD4。4株毒株经病毒蚀斑克隆纯化后动物回归试验可产生典型的NDV病变|为了解其遗传起源和分子特性对其融合蛋白(Fusion,F)进行序列和分子特性的分析。研究结果发现,生物学毒力测定显示4株毒株均为强毒。F蛋白多肽裂解位点的序列为112RRQKRF117,符合NDV强毒氨基酸基序。F基因分型显示,SD01、SD02、SD03和SD04与另外一株鸡源SG01以及广东鹅分离株等属于基因Ⅶ型。同源性比较显示:SD1-SD4与同期分离株的同源性明显高于其他的传统毒株,且与国内分离株的同源性高于国外的分离株。
Four Newcastle Disease Virus isolates named SD01-SD04 were collected from chicken in Shandong in 2011. The four strains were purified by plaque assay can form typical cytopathic. The Fgenes of the isolates were cloned and sequenced to study its molecular characterization and hereditary variation. The deduced amino acid se-quence of Fgene showed that these viruses shared a velogenic motif 112RRQKRF 117at the cleavage site which were coincident with biological index. Phylogenetic analysis showed that SD01-SD04 and the viruses derived from chicken andgoose in China belonged togenotype Ⅶ. For Fgene of the isolates,the homologies of the deduced amino acid se-quences of SD01-SD04 with the same time isolates in China were higher compared with traditional trains. There was a closergenetic relationship between the four isolates and epidemic strains in China than foreign epidemic strains.
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