以抗病资源收集一号和感病农家品种皇后杂交的F1、BCs、BCr、F2以及双亲为材料,苗期进行白粉病抗性鉴定,分析了白粉病抗源收集一号的抗性遗传规律,并利用集团分离分析法结合SRAP标记进行连锁分析。结果表明,收集一号对白粉病Podosphaera xanthii生理小种1的抗性由显性单基因控制,同时对1 600对SRAP引物进行筛选,引物me4em37扩增出的多态性条带与抗病基因表现连锁关系,该多态性片段大小为100 bp,与抗病基因遗传连锁距离为17.0 cM。
Parental lines and(No. 1Shouji×Huanghou)F1,F2and backcross populations were scored for re-sponse to inoculation with Podosphaera xanthiirace 1. The sequence related amplified polymorphism(SRAP)tech-nique and bulk segregant analysis(BSA)were used to identify molecular markers linked to the resistance of melon powdery mildew. The resistance to P. xanthii race 1 in No. 1 Shouji was controlled by one single dominantgene. A total 1 600 pairs SRAP primers could detect the polymorphism between the resistant pool and susceptible pool. An unique 100 bp fragment was amplified with the primer me4em37. It was identified that is linked to resistancegene of No. 1 Shouji at a distance of 17. 0 cM.
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