论文

植物病原细菌hrp基因簇比较基因组分析

  • 陶晡 ,
  • 刘彬 ,
  • 司贺龙 ,
  • 赵斌 ,
  • 邢继红 ,
  • 董金皋
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  • 河北农业大学, 真菌毒素与植物分子病理学实验室, 河北 保定 071001
陶晡(1981-),男,河北正定人,助理研究员,硕士,主要从事生物信息学研究。陶晡、刘彬为同等贡献作者。

收稿日期: 2013-01-09

  网络出版日期: 2014-10-14

Bioinformatics Analyse the hrpgene Clusters of Phytopathogenic Bacteria

  • TAO Bu ,
  • LIU Bin ,
  • SI He-long ,
  • ZHAO Bin ,
  • XING Ji-hong ,
  • DONG Jin-gao
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  • Agricultural University of Hebei, Mycotoxin and Moleculer Plant Pathology Laboratory, Baoding 071001, China

Received date: 2013-01-09

  Online published: 2014-10-14

摘要

hrp基因是植物病原细菌中一类重要的致病基因,一般成簇排列。对14种已完成全基因组测序的植物病原细菌基因组中的hrp基因簇进行了大小、G+C含量、基因组成的BlastP比较。发现它们的大小在18~24 kb,由22~28个基因组成。欧文氏菌属和假单胞菌属的菌株中G+C含量较低,拉尔氏菌属和黄单胞菌属菌株的G+C含量较高。而且,各菌属的基因簇都有其独有的hrp基因,如假单胞菌属是hrpK1、hrpV、hrpA|欧文氏菌属是hrpT、hrpV、hrpA|拉尔氏菌属是hrpZ、hrpX、hrpY、hrpV|黄单胞菌属是hrpE、hrpD6、hpaA。其中,基因hrpA1、hrpA、hrpY和hrpE在假单胞菌属、欧文氏菌属、拉尔氏菌属和黄单胞菌属中均编码Ⅲ型菌毛的基因,可能在寄主和病原物识别中起重要作用。

本文引用格式

陶晡 , 刘彬 , 司贺龙 , 赵斌 , 邢继红 , 董金皋 . 植物病原细菌hrp基因簇比较基因组分析[J]. 华北农学报, 2013 , 28(2) : 27 -31 . DOI: 10.3969/j.issn.1000-7091.2013.02.005

Abstract

hrpgene is one of important pathogenitygenes in phytopathogenic bacteria,usually in cluster. The size,g+ C contents and BlastP of the constitutedgenes of 14 completed sequenced phytopathogenic bacterias in this article compare. It find that these size ofgene clusters among 18-24 kb,made up by 22-28genes. Theg+ C con-tents is lower in the Pseudomonas and Erwiniagenus,but higher in the Xanthomonas and Ralstoniagenus. Moreo-ver,everygenus has its own unique hrpgene cluster,such as in the Pseudomonas are hrpZ1,hrpA1,hrpVgenes. In the Erwiniagenus are hrpT,hrpV,hrpAgenes. In the Ralstoniagenus are hrpZ,hrpX,hrpY,hrpVgenes. In the Xan-thomonas are hrpE,hrpD6,hpaA. Among thesegenes,hrpA1,hrpA,hrpY,hrpE respectively belong to Pseudomonas spp,Erwinia spp,Ralstonia spp and Xanthomonas spp,encode type Ⅲ pilus,thesegenes maybe act as important function in pathogens recognize host progross.

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