论文

梨AFLP分子连锁图谱的构建与分析

  • 孙文英 ,
  • 张玉星 ,
  • 李秀根 ,
  • 王龙
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  • 1. 河北农业大学园艺学院, 河北保定 071001;
    2. 中国农业科学院郑州果树研究所, 河南郑州 450009
孙文英(1979-), 女, 河北衡水人, 博士, 主要从事果树结实生理与分子生物学的研究.

收稿日期: 2008-12-22

  网络出版日期: 2014-10-14

基金资助

河北省自然科学基金(303240);河北农业大学"将帅"计划基金

Construction and Analysis of AFLP Molecular Linkage Map in Pear

  • SUN Wen ying ,
  • ZHANG Yu xing ,
  • LI Xiu gen ,
  • WANG Long
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  • 1. College of Horticulture,Agricultural University of Hebei,Baoding 071001,China;

    2. Zhengzhou Fruit Institute,Chinese Academy of Agriculture Science,Zhengzhou 450009,China

Received date: 2008-12-22

  Online published: 2014-10-14

摘要

遗传图谱是数量性状基因定位、基因图位克隆及分子辅助育种等研究的基础,而目前国内在梨树上这方面的研究尚属空白.试验以早美酥×八月红的杂交F1 92株实生苗为作图群体,采用了扩增稳定、多态性丰富的16对Mse I/EcoR I引物组合,对分离群体进行AFLP多态性检测,共获得208条多态性带,其中偏离孟德尔遗传的比率29.8%(P<0.01).应用Joinmap 3.0软件且结合"双假测交"策略,对208个AFLP分子标记进行了遗传连锁分析,构建了一张包含14个AFLP标记,分属20个连锁群的梨分子遗传连锁图谱,每个连锁群包含4~20个标记,平均为9.06个标记,图谱总长度覆盖梨基因组618.7 cM,标记间平均图距为6.8 cM.此图谱为梨树基因定位、比较基因组学和重要农艺性状的QTL定位等研究奠定了基础.

关键词: ; 分子连锁图谱; AFLP

本文引用格式

孙文英 , 张玉星 , 李秀根 , 王龙 . 梨AFLP分子连锁图谱的构建与分析[J]. 华北农学报, 2009 , 24(3) : 179 -183 . DOI: 10.7668/hbnxb.2009.03.038

Abstract

Genetic linkage map was the foundation of locating quantitative trait loci,genes map-based cloning and marker assisted selection, while the research had done little on the pear. In order to accelerate the molecular breeding study on pear,a genetic map for pear( Pyrus L. ) has been developed based on 92 F1plants derived from an interspecific cross between the cultivars Zaomeisu and Bayuehong. The linkage map was constructed based on 208 polymorphic AFLP markers and the ″ double pseudo-testcross″strategy by using Jionmap version 3. 0 software. The map in this study covered 618. 7 cM of pear genome harboring 145 AFLP markers on the twenty linkage groups,which the length of linkage group varied from 2. 06 cM to 79. 3 cM and an average genetic distance between adjacent markers was about 6. 58 cM of Kosambi genetic distance. And also the markers on the linkage group varied from 4 to 20. Compared with the reports of pear genetic maps,the pear linkage map in this study was not saturated, but it was currently the first integrated linkage map in pear comprising 47 3∶ 1 and 98 1∶ 1AFLP markers by using Joinmap 3. 0 setting CP( cross pollination) type data.

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