论文

产胞外淀粉酶枯草芽孢杆菌的分离筛选及其紫外诱变育种

  • 谢凤行 ,
  • 赵玉洁 ,
  • 周可 ,
  • 张峰峰 ,
  • 李亚玲
展开
  • 天津市农业生物技术研究中心, 天津 300192
谢凤行(1979-), 女, 湖南邵阳人, 硕士, 主要从事微生物发酵研究工作.

收稿日期: 2008-11-14

  网络出版日期: 2014-10-14

基金资助

天津市应用基础研究计划项目(07JCZDJC03600);天津市农业科学院院长基金项目(07012)

The Isolation,Screening and Ultraviolet Mutation Breeding of Bacillus subtilis Strains Producing Extracellular Amylase

  • XIE Feng xing ,
  • ZHAo Yujie ,
  • ZHoU Ke ,
  • ZHANG Feng feng ,
  • LI Ya ling
Expand
  • Tianjin Research Center of Agricultural Biotechnology,Tianjin 300192,China

Received date: 2008-11-14

  Online published: 2014-10-14

摘要

为提高枯草芽孢杆菌产生淀粉酶能力,从养虾池、混养池及污染河流的底质活性污泥中分离到12株枯草芽孢杆菌,通过淀粉降解试验筛选到产酶能力较高的菌株H4和H,菌株淀粉酶活性分别为38.66,37.10 U/mL.以筛选到H4和H为原始菌株,采用紫外诱变的方法对H4和H进行连续诱变筛选产淀粉酶高的突变株.结果发现,第一次诱变后突变株的酶活分别为原始菌株的107%和111%;经过第二次诱变后,H4的突变株H4 Ⅱa,H的突变株HⅡa和HⅡb的产酶能力有很大程度的提高,分别为原始菌株的147%,136%和13%,酶活达6.9,0.47和0.02 U/mL,说明紫外连续诱变有利于突变株产酶能力的提高.

本文引用格式

谢凤行 , 赵玉洁 , 周可 , 张峰峰 , 李亚玲 . 产胞外淀粉酶枯草芽孢杆菌的分离筛选及其紫外诱变育种[J]. 华北农学报, 2009 , 24(3) : 78 -82 . DOI: 10.7668/hbnxb.2009.03.017

Abstract

Bacillus subtilis used as probiotic was widely applied to the aquiculture to purify the water with the needs of healthy breeding. In this paper twelve Bacillus subtilis strains were isolated from activate sludge of breeding shrimp pond,breeding fish pond and polluted river. H4and H5with high amylase activity were screened from the isolated strains by amylum degradation experiment and the activities of amylase were 38. 66,37. 10 U/ mL,respectively. The mean of ul2 traviolet continuous induced mutation was used on H4and H5. The results showed that the extracellular amylase activities of first mutant strains H4Ⅰ and H5Ⅰ were 41. 36 and 41. 32 U/ mL,which were 107 % and 111 %of initial strains. While the extracellular amylase activities of second mutant strains H4Ⅱ a,H5Ⅱ a and H5Ⅱ b were increased to 147 %,136 % and 135 % of initial strains,and the activities of amylase were 56. 95,50. 47 and 50. 02 U/ mL,respectively. The results indicate that ultraviolet continuous induced mutation would be in favor of the amylase producing of mutant strain.

参考文献

[1] 祁真,杨京平.几种微生物制剂和微藻在水产养殖中的应用[J].水生生物学报,2004,28(1):85-89.
[2] 蒋若天,卢涛,宋航,等.一株a-高温淀粉酶的地衣芽孢杆菌产酶培养基的优化[J].现代食品科技,2006,4:52-54.
[3] 杨慧,王振华,潘康成.芽孢杆菌产淀粉酶活性的研究[J].现代食品科技,2007,2:90-93.
[4] 孟立花,李社增,郭庆港,等.枯草芽孢杆菌NCD-2菌株抗菌蛋白初步分析(J].华北农学报,2008,23(1):189-193.
[5] 赵达,刘伟成,裘季燕,等.枯草芽孢杆菌B03液体发酵条件的优化[J].华北农学报,2008,23(2):205-209.
[6] 杜立新,冯书亮,王容燕,等.拮抗BS-208菌株对番茄灰霉病诱导抗性的初步研究[J].华北农学报,2005,20(6):84-87.
[7] 孙瑶,马平,朱宝成,等.棉花黄萎菌拮抗菌BDT-25的鉴定及抗菌蛋白产生条件研究[J].华北农学报,2006,21(6):119-123.
[8] 胡咏梅,葛向阳,梁运祥.枯草芽孢杆菌FY99201菌株的净水作用[J].华中农业大学学报,2006,25(4):404-407.
[9] 雷爱莹,彭敏,曾地刚,等.枯草芽孢杆菌的分离和净化水质的研究[J].广西农业科学,2006,36(3):248-250.
[10] Vaseeharan B,Ramasaray P.Control of pathogenic Vibrio spp.by Bacillus subtilis BT23,a possible probiotic treatment for black tiger shrimp Penaeus monodon[J].Letters in Ap-plied Microbiology,2003,36(2):83-87.
[11] Salinas I,Cuesta A,Esteban M A,et al.Dietary administra-tion of Lactobacill delbrueckii and Bacillus subtilis,single or combined,on giltllead seabream cellular innate immune re-sponses[J].Fish and Shellfish Immunology,2005,19(1):67-77.
文章导航

/