为解决奶白菜杂交种生产中的杂交制种手段问题,配制优良杂交种。以核不育"复等位基因遗传"假说为指导,以青梗白菜核基因雄性不育系00S107作不育源,采用连续回交转育同时测交鉴定基因型的方法,定向转育奶白菜核基因雄性不育系。并利用转育成的不育系与奶白菜优良自交系配制杂交组合,进行了杂种优势分析。选育出园艺学性状与目标品系相似,具有100%不育株率和不育度的奶白菜核基因雄性不育系GMS3,筛选出2个产量高、整齐度高的优异杂交组合GMS3×B1、GMS3×B2。定向转育模式兼顾了不育基因和园艺学性状的转育,解决了奶白菜核不育系转育和利用的难题。
In oRdeR to Resolve the pRoblem that cRoss bReeding and pRoduction of hybRid in milk Chinese cabbage. AccoRding to the hypothesis of multiple allele inheRited genetic male steRile,A genetic male steRile line of Chinese cabbage with gReen stipe 00S107 was used as a souRce of male steRility,and methods of continuous backcRossing and identifying of the genotypes weRe applied to tRansfeR the male steRile line of milk Chinese cabbage. HybRidized combinations weRe obtained by the male steRile line as female paRent cRoss with excellent self-line and we analysed the hybRid vigoR. A new genetic male steRile line GMS3 with 100% male steRile plants and 100% male steRility was obtained. Two excellent hybRidized combinations GMS3 × B1 ,GMS3 × B2 weRe selected,which is unifoRm and supeRioR to CK in yield. DiRectional tRansfeR model which tRansfeR steRile gene and hoRticultuRal chaRacteRs at the same time Resolved the puzzle that bReeding and utilization of the genetic male steRile line in milk Chinese cabbage.
[1] 冯辉,魏毓棠,许明. 大白菜核基因雄性不育的复 等位基因假说[C]. 辽宁省第二届青年学术年会论文 集. 大连: 大连理工大学出版社, 1995: 149 - 153.
[2] Feng H,Wei Y T,Xu M. Multiple allele model foR genetic male steRility in Chinese cabbage[J]. Acta HoRticultuRae, 1996, 467: 133 - 142.
[3] 闻凤英,宋连玖,王玉龙,等. 青麻叶结球白菜雄性不 育系的转育[J]. 园艺学报, 2001, 28( 2) : 133 - 138.
[4] 吕艳玲,陶承光,王鑫. 利用杂交种转育大白菜核基 因雄性不育系的研究[J]. 华北农学报,2008,23( 3) : 31 - 33.
[5] 逯保德,赵美华,兰创业. 大白菜细胞质雄性不育系选 育研究[J]. 山西农业科学, 2009, 37( 5) : 24 - 27.
[6] 张德双,张凤兰,王永健,等. 大白菜细胞质雄性不育 的分子鉴定及序列分析[J]. 华北农学报,2007,22 ( 6) : 53 - 59.
[7] 杨皓宁,冯辉,李承. 大白菜核不育复等位基因的 鉴定与分布[J]. 河南农业科学, 2008( 4) : 87 - 90.
[8] 许明,魏毓棠,白明义,等. 大白菜显性核基因雄性 不育性向紫菜薹的转育初报[J]. 园艺学报,2003,30 ( 1) : 98 - 100.
[9] 许明,白明义,魏毓棠. 大白菜细胞核雄性不育基因向 自交系97A407 的转育[J]. 中国蔬菜, 2003( 2) : 8 -10.
[10] 岳艳玲,冯辉. 核基因雄性不育在不同生态型大白 菜间的转育[J]. 中国蔬菜, 2005( 7) : 22 - 23.
[11] 岳艳玲,冯辉. 核雄性不育基因向卵圆生态型大白 菜中的成功转育[J]. 沈阳农业大学学报,2005,36 ( 5) : 603 - 605.
[12] 冯辉,王玉刚,林桂荣,等. 大白菜细胞核雄性不育 基因向小白菜中转育的研究[J]. 河北科技师范学 报, 2004( 6) : 10 - 13.
[13] 张庶,周新成,李利斌,等. 利用EST-SSR 标记鉴定 大白菜杂交种纯度的研究[J]. 天津农业科学, 2010, 16( 6) : 1 - 4.
[14] 闫世江,赵俊. 大白菜杂种优势预测研究[J]. 山西 农业科学, 2007, 35( 1) : 33 - 35.