为了对转基因植物源饲料的安全性提供依据,针对转基因豆粕中的CaMV3S启动子和NOS终止子设计引物,利用PCR技术研究加热、高压蒸汽、膨化以及制粒加工工艺对豆粕中启动子及终止子片段长度的影响.结果表明,加热处理对豆粕中启动子基因和终止子基因的片段长度均没有影响;但高压蒸汽处理对豆粕中对启动子基因和终止子基因的影响明显不同,启动子基因的246,16和101 bp这3个片段在处理后均可被检测到,而终止子基因只有12 bp的片段能被检测到,217 bp片段在处理后检测不到;对以豆粕为原料的饲料产品进行检测也得到相同的结果,即启动子基因较为稳定,终止子基因则较易被破坏.
In order to privide some knowledge for transgenic plant feed, primeres were designed on transgenic soy2 bean meal within CaMV35 S promoter gene and NOS terminator, and effect of heat treatment, autoclave, and other process2 ing technics on degradation of CaMV35 S and NOS in soybean meal was investigated by polymerase chain reaction detec2 tion system in this experiment.The results showed that heat treatment had no effect on degradation of both CaMV35S and NOS.Autoclave treatment also did not affect degradation of CaMV35S,and 246,165,and 101 bp fragments of CaMV35S were all detectable,but resulted in degradation of NOS,and 125 bp fragment was detectable and 217 bp fragment was undetectable.And the same result was obtained also in feeds those processed fromsoybean meal.The promoter gene was relatively stable and terminator gene was relatively easily destroyed.
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