论文

氮素供应水平对小粒型花生氮素代谢及相关酶活性的影响

  • 张智猛 ,
  • 万书波 ,
  • 孙奎香 ,
  • 戴良香
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  • 1. 山东省花生研究所, 山东 青岛 266100;
    2. 山东省农科院, 山东 济南 250100
张智猛( 1963- ), 男,河北衡水人, 研究员, 博士, 主要从事植物栽培生理学研究.

收稿日期: 2008-12-15

  网络出版日期: 2014-10-14

基金资助

国家农业科技成果转化资金项目(03EFN213710259);国家科技支撑计划(2006BAD21B04-2)

Effects of Nitrogen Level on Nitrogen Metabolism and Correlating Enzyme Activity of Peanut

  • ZHANG Zhi-meng ,
  • WAN Shu-bo ,
  • SUN Kui-xiang ,
  • DAI Liang-xiang
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  • 1. Peanut Research Institute of Shandong Province, Qingdao 266100, China;
    2. Shandong Academy of Agriculture Sciences, Jinan 250100, China

Received date: 2008-12-15

  Online published: 2014-10-14

摘要

花生籽仁蛋白质含量较高,研究不同品种花生各器官中氮代谢酶活性的差异及与籽仁蛋白质含量间的关系,可为花生优质高产提供依据.在大田高产条件下研究了氮素水平对小粒型花生各器官中可溶性蛋白质、游离氨基酸含量及氮代谢相关酶活性的影响,结果表明,适当提高氮素水平既能增加花生各器官中可溶性蛋白质和游离氨基酸的含量,又能提高硝酸还原酶、谷氨酰胺合成酶和谷氨酸脱氢酶等氮素同化酶的活性,使其达到同步增加;氮素水平过高虽能提高硝酸还原酶和籽仁蛋白质含量,但谷氨酰胺合成酶和谷氨酸脱氢酶的活性下降;N素施肥水平不改变花生植株各器官中硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、谷氨酸脱氢酶(GDH)活性的变化趋势,但适量施N(B2、B3处理)使花生各营养器官中NR、GS活性提高;氮素水平对花生各叶片和籽仁中GDH活性的高低影响较大,但对茎和根中活性大小的影响则较小.

本文引用格式

张智猛 , 万书波 , 孙奎香 , 戴良香 . 氮素供应水平对小粒型花生氮素代谢及相关酶活性的影响[J]. 华北农学报, 2009 , 24(2) : 170 -175 . DOI: 10.7668/hbnxb.2009.02.035

Abstract

The effects of nitrogen level on soluble protein content, free amino acid content and correlating enzyme activity of nitrogen metabolism of peanut was studied in the field.The results showed that: with the increase of nitrogen,the soluble protein content and free amino acid content increased, and the activities of the nitrate reductase( NR),glutamine synthetase(GS) and glutamate dehydrogenase(GDH) also increased at the same time;when the excessive nitrogen used,the NR act ivity and kernel protein content were increased, while the activities of GS and GDH were decreased;the change trends of NR,GS and GDH were not affected by the nitrogen level,but with suitable nitrogen the activit ies of NR and GS in different organs could be increased; and the nitrogen level effected the GDH activity in leaf and kernel more obviously,with lower effects on its activity in stalk and root.

参考文献

[1] Alloush G A,Bot J Le,Sanders F E.Mineral nutrition of chickpea plants supplied with NO3 or NH3-N ionic balance in relation to irons tress[J].Journal of Plant Nutrition,1990,13(12):1575-1590.
[2] Beusichem M L,Kirkby E A,Baas R.Influence of nitrate and ammonium nutrition on the uptake,assimilation and distribution of nutrients in ricinus communis[J].Plant Physiology,1988,86(3):914-921.
[3] 柴小清,印莉萍,刘祥林,等.不同浓度的NO3+和NH4+对小麦根谷氨酰胺合成酶及其相关酶的影响[J].植物学报,1996,38(10):803-808.
[4] 黄勤妮,印莉萍,柴晓清,等.不同氮源对小麦幼苗谷氨酰胺合成酶的影响[J].植物学报,1995,37(11):856-868.
[5] 王月福,于振文,李尚霞,等.氮素营养水平对冬小麦氮代谢关键酶活性变化和籽粒蛋白质含量的影响[J].作物学报,2002,28(6):743-748.
[6] 王小纯,熊淑萍,马新明,等.不同形态氮素对专用型小麦花后氮代谢关键酶活性及籽粒蛋白质含量的影响[J].生态学报,2005,25(4):802-807.
[7] 张智猛,戴良香,胡昌浩,等.氮素对不同类型玉米蛋白质及其组分和相关酶活性的影响[J].植物营养与肥料学报,2005,11(3):320-326.
[8] 王宪泽,程炳嵩,张国珍.氮素形态与作物生育的关系及其影响因子[J].山东农业大学学报,1990,21(1):93-98.
[9] Rhodes D,Rendon G A,Stewart D R.The regulation of ammonia assimilating enzymesin lemna minor[J].Planta,1976,129:203-210.
[10] 曹云,范晓荣,贾莉君,等.不同水稻品种对NO3-同化差异的比较[J].南京农业大学学报,2005,28(1):52-56.
[11] 陈煜,朱保葛,张敬,等.不同氮源对大豆硝酸还原酶和谷氨酰胺合成酶活性及蛋白质含量的影响[J].大豆科学,2004,23(4):143-146.
[12] 张宏纪,马凤鸣,李文华,等.不同形态氮素对甜菜谷氨酰胺合成酶活性的影响[J].黑龙江农业科学,2001(6):7-10.
[13] 朱长甫,苗以农,杨文杰,等.大豆种子蛋白质含量与固氮酶活性和硝酸还原酶活性的关系[J].中国油料作物学报,1992,14(2):29-32.
[14] 李彩凤,马风呜,赵越,等.氮素形态对甜菜氮糖代谢关键酶活性及相关产物的影响[J].作物学报,2003,29(1):128-132.
[15] 王云华,王志强,张楚富,等.硝态氮对黄瓜子叶谷氨酰胺合成酶和谷氨酸脱氢酶活性的影响[J].武汉植物学研究,2004,22(6):534-538.
[16] 肖昌珍,吴渝,甘冬生,等.中国花生种质主要生化品质分析[J].花生科技,1999(增):161-167.
[17] 王才斌,孙彦浩,牛振荣,等.高产花生叶片和荚果谈碳氮化合物代谢规律的研究[J].莱阳农学院学报,1996,13(2):99-104.
[18] 左元梅,刘永秀,张福锁.玉米、花生混作改善花生铁营养对花生根瘤碳氮代谢及固氮的影响[J].生态学报,2004,24(11):2584-2590.
[19] 姜慧芳,任小平.干旱胁迫对花生叶片SOD活性和蛋白质的影响[J].作物学报,2004,30(2):169-174.
[20] 聂呈荣,不同氮肥和调节膦对花生氮代谢与产量的作用[J].中国油料,1996,18(2):31-33.
[21] 聂呈荣,凌菱生.花生不同密度群体施用植物生长调节剂对生长发育和氮素代谢的影响[J].中国油料作物学报,1998,20(4):47-51.
[22] 杜应琼,廖新荣,黄志尧,等.硼、钼对花生氮代谢的影响[J].作物学报,2001,27(5):612-616.
[23] 上海植物生理学会,植物生理学实验手册[M].上海:上海科学技术出版社,1999:115-150.
[24] Cren M,Hirel B.Glutamine synthetase in higher plant:regulation of gene and protein expression from theorgan to the cell[J].Plant cell Physiology,1999,40:1187-1193.
[25] 王月福,姜东,余振文,等.氮素水平对小麦籽粒产量和蛋白质含量的影响及其生理基础[J].中国农业科学,2003,36(5):513-520.
[26] 荆奇,曹卫星,戴廷波.小麦籽粒品质形成及其调控研究进展[J].麦类作物学报,1999,19(4):46-50.
[27] 张翔,焦有,孙春河,等.不同施肥结构对花生产量和品质的影响[J].土壤肥料,2003(2):30-32.
[28] 甄志高,段莹,王晓林,等.长期定位施肥对花生产量和品质的影响[J].土壤通报,2006,37(2):323-325.
[29] 范雪梅,姜东,戴廷波,等.不同水分条件下氮素供应对小麦植株氮代谢及籽粒蛋白质积累的影响[J].生态学杂志,2006(2):323-325.
[30] Thomas W Becker,Elisa Carrayol,Bertrand Hirel.Glutamine synthetase and glutamatede hydrogenase is formsin maize leaves:localization,relative ropertion and the irrolein ammonium assimilation omitrogen transport[J].Planta,2000,(211):800-806.
[31] Miflin B J,Habash D Z.The role of glutamine synthetase and glutamate dehydrogenase in nitrogen assimilation and possibilities for improvement in the nitrogen utilization of crops[J].J Exp Bot,2002,53:979-987.
[32] 欧吉权,魏国威,张楚富,等.甜瓜子叶发育过程中蛋白质水平和氨同化酶活性变化[J].武汉大学学报:理学版,2003,49(4):538-542.
[33] Len Edela,Haba P,Maldonado J M.Changes in the Levels of enzymes involved in ammonia assimilation during the dvelopment of phaseolus vulgaris seedlings[J].Effects of Exogenous Ammonia Physiol Plant,80:20-26.
[34] 李常军,宫长荣,陈江华,等.烘烤湿度条件对烟叶氮代谢的影响[J].华北农学报,2001,16(2):141-144.
[35] 韩锦峰,汪耀富,岳翠凌,等.干旱胁迫下烤烟光合特性和氮代谢研究[J].华北农学报,1994,9(2):39-45.
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