以笋王一号莴苣为试验材料,研究了不同浓度Cd胁迫对莴苣种子萌发、胚根胚芽生长、丙二醛(MDA)及抗氧化酶系统的影响.结果表明:随着Cd浓度的增加,莴苣种子的发芽率逐渐降低,而Cd对种子的发芽势、发芽指数和活力指数以及胚根的伸长则表现出低促高抑的效应.Cd对胚根生长的抑制程度较胚芽明显.随着Cd浓度的增加.胚根中MDA含量、SOD、G-POD活性明显增加,APX和CAT活性则明显降低,而胚芽中只在高浓度Cd胁迫下(100μmol/L)才发生明显变化.
The effects of different Cadmium(Cd) concentration on seed germination,the growth of radicle and plumula,melondialodehyde (MDA) and the activities of antioxidant enzymes were investigated. The results showed germination rate decreased with the increasing of Cd concentration. Germination potential,germination index,vigour index and the length of radicle were promoted at lower Cd concentration and inhibited at higher Cd concentration. Moverover,Cd had more inhibition effects on radicle growth than plumula.The content of MDA and activities of SOD and G- POD in radicle increased significantly with the increasing of Cd concentration,whereas,the activities of APX and CAT decreased markedly.The content of MDA and the activities of SOD,G- POD,APX,CAT did not change significantly except at the higher Cd concentration (100μmol/L).
[1] Clemente R,Walker D J,Roig A,et al.Heavy metal bioavailability in a soil affected by mineral sulphides contamination following the mine spillage at Aznalcollar (Spain)[J].Biodegradation,2003,14 (3):199-205.
[2] 顾继光,林秋奇,胡韧,等.土壤-植物系统中重金属污染的治理途径及其研究展望[J].土壤通报,2005,36(1):128-133.
[3] 杨居荣,贺建群,张国祥,等.作物对Cd毒害的耐性机理探讨[J].应用生态学报,1995,6(1):87-91.
[4] Das P,Samantaray S,Rout G R.Studies on cadmium toxicity in plants:a review[J].Environ Poilu,1998,98:29-36.
[5] 郑春荣,孙兆海,周东美,等.土壤Pb、Cd污染的植物效应Ⅱ.Cd污染对水稻生长和Cd含量的影响[J].农业环境科学学报,2004,23(5):872-876.
[6] 马文丽,金小弟,王转花.镉处理对乌麦种子萌发幼苗生长及抗氧化酶的影响[J].农业环境科学学报,2004,23(1):55-59.
[7] 何俊瑜,任艳芳.镉胁迫对水稻种子萌发、幼苗生长和淀粉酶活性的影响[J].华北农学报,2008,23(增刊):131-134.
[8] 任安芝,高玉葆.铅、镉、铬单一和复合污染对青菜种子萌发的生物学效应[J].生态学杂志,2000,19(1):19-22.
[9] 秦秀昌,郭秀璞,史国安.镉对小麦种子萌发和幼苗生长的影响[J].麦类作物学报,2002,22(3):89-91.
[10] 宋玉芳,许华夏,任丽萍,等.土壤重金属对白菜种子发芽与根伸长抑制的生态毒性效应[J].环境科学,2002,23(1):103-107.
[11] 陈静波,田迪英.莴笋不同部位抗氧化活性的研究[J].食品研究与开发,2006,27(9):54-57.
[12] 张志良,瞿伟.植物生理学实验指导[M].北京:高等教育出版社,2003.
[13] Klapheck S,Zimmer I,Cossse H.Scavenging peroxide in the endosperm of Ricinus communis peroxidase[J].Plant Cell Physiol,1990,31:1005-1013.
[14] Nakano Y,Asada K.Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts[J].Plant Cell Physiol,1981,22:867-880.
[15] Aebi H.Catalase in vitro[J].Method Enzymol,1984,105:121-126.
[16] 张春荣,李红,夏立江,等.镉、锌对紫花苜蓿种子萌发及幼苗的影响[J].华北农学报,2005,20(1):96-99.
[17] 何宇清,王炳锐,王建波.镉胁迫下小麦根尖分生细胞中Ca2+分布的变化[J].武汉植物学研究,2002,20(6):417-420.
[18] 吴启堂,陈卢,王广寿.水稻不同品种对Cd吸收累积的差异和机理研究[J].生态学报,1999,19(1):104-107.
[19] 何俊瑜,任艳芳,朱诚,等.镉胁迫对不同水稻品种种子萌发、幼苗生长和淀粉酶活性的影响[J].中国水稻科学,2008,22(4):409-414.
[20] Shah K,Kumar R G,Verma A,et al.Effect of cadmium on lipid peroxidation,superoxide anion generation and activities of antioxidant enzymes in growing rice seedlings[J].Plant Science,2001,161:1135-1144.
[21] 吴旭红.三个苜蓿品种对镉污染的生理生态反应及抗性比较[J].生态环境,2005,14(5):658-661.
[22] 李元,王焕校,吴玉树.Cd、Fe及其复合污染对烟草叶片几项生理指标的影响[J].生态学报,1992,12(2):147-154.
[23] Hall J L.Cellular mechanisms for heavy metal detoxification and tolerance[J].J Exp Bot,2002,53(366):1-11.