以番茄无菌苗的子叶和下胚轴为外植体,通过农杆菌介导法,对其遗传转化条件进行了优化,建立了高效番茄子叶和下胚轴农杆菌介导的遗传转化体系.结果表明:在农杆菌浸染前(浸染浓度为OD600=0.3)进行2 d的预培养浸染6 min的外植体在MS+210 mg/L6-BA+015 mg/L IAA的培养基上转化效率最高.PCR检测初步证明,NPTII基因已整合到番茄再生植株中.
Tomato(Lycopersicon esculentum Mill ) cultivars,Texuan-28,Taiyuan 823were used as the materials,and cotyledon and hypocotyls were cultured to optimize the transformation system via Agrobacterium.The results showed that the highest transformation efficiency was obtained when the explants were preconditioned on preconditioning medium for 2 d prior to infect with Agrobacterium (OD600= 013) for 6 min growing on the culture mediumof MS+ 210mg/L 6-BA+015 mg/L IAA.It was proved that the target NPTII gene had been integrated into the genome of regenerated plants by PCR analysis.
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