论文

柞蚕核型多角体病毒p11基因克隆及序列分析

  • 石生林 ,
  • 刘彦群 ,
  • 潘敏慧 ,
  • 鲁成
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  • 1. 沈阳农业大学 生物科学技术学院, 辽宁 沈阳 110161;
    2. 西南大学 农业部蚕桑学重点开放实验室, 重庆 400716
石生林( 1972- ), 男, 辽宁岫岩人, 副教授, 理学博士, 主要从事昆虫病原分子生物学相关研究.

收稿日期: 2008-12-09

  网络出版日期: 2014-10-14

基金资助

沈阳农业大学中青年研究生导师基金资助(2006):国家自然科学基金资助(30800803;30771630)

Cloning and Sequence Analysis of Antheraea pernyi Nucleopolyhedrovirus (ApNPV) p11 Gene

  • SHI Sheng-lin ,
  • LIU Yan-qun ,
  • PAN Min- hui ,
  • LU Cheng
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  • 1. College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110161, China;
    2. Key Sericultural Laboratory of Agriculture Ministry, Southwest University, Chongqing 400716, China

Received date: 2008-12-09

  Online published: 2014-10-14

摘要

为丰富柞蚕核型多角体病毒分子生物学基础,通过PCR扩增克隆了ApNPV p11基因并进行了序列的生物信息学分析.ApNPV p11基因编码102个氨基酸,预测分子量11.2 kDa;氨基酸序列N端1~33位是信号肽序列,中部0~72位是跨膜区.PSI-BLAST搜索表明有20种核型多角体病毒编码蛋白与ApNPV P11蛋白有显著性匹配;比对分析发现P11蛋白氨基酸序列中部相对保守,两端变异较大.进化分析表明ApNPV属于NPV类群Ⅰ且与EppoNPV、AgMNPV、CfDefNPV亲缘关系较近.

本文引用格式

石生林 , 刘彦群 , 潘敏慧 , 鲁成 . 柞蚕核型多角体病毒p11基因克隆及序列分析[J]. 华北农学报, 2009 , 24(2) : 42 -46 . DOI: 10.7668/hbnxb.2009.02.009

Abstract

To enrich the molecular biology information of Antheraea pernyi nucleopolyhedrovirus( ApNPV),the p11 gene was PCR amplified and cloned. Moreover,the sequence was subjected to bioinformatics analysis. ApNPV p11 gene encoded a 102 amino acid sequence with a predicted molecular weight of 11.2 kDa.The position 1-33 of the N terminal was the signal peptide sequence and the middle position(50-72) was the transmembrane domain.PSI- BLAST search revealed that total 20 nucleopolyhedrovirus proteins significantly match with ApNPV P11 protein.Align analysis indicated that the middle parts was relatively conserved and the two ends were less conserved. Phylogenetic analysis classified ApNPV as one group I NPV and closely related to EppoNPV,AgMNPV and CfDefNPV.

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