利用SSR分子标记技术以及软件Mapmaker3.0,Mapdraw2.0和QTLMaper1.6对齐319和掖478及其F2∶3家系进行了初步的遗传分析,构建了分子遗传连锁图谱.该连锁图总长度1 638.7 cM,包含114个标记,平均两个标记间的距离为14.37 cM.在所做标记的附近得到个QTL,分别位于第2,3,,6,9条染色体上,能解释表型变异的36.21%,21.6%,39.41%,20.67%,30.4%,而且均检测出加性效应.另外,还发现QTLs之间表现出上位性互作.
In this study,SSR molecular marker technology and software Mapmaker3.0,Mapdraw2.0 and alignment QTLMaper1.6 were applied for preliminary genetic analysisof Ye 478,Qi319 and populationof F2:3 lines,and molecular genetic linkage map was constructed. The total length of the linkage map was 1638.7 cM,containing 114 markers,and the average distance between two markerswas 14.37 cM. Five QTLswerefound near the SSR markers set,whichwere localizedon chromosome 2,3,5,6,9,which could explain 36121 %,2116 %,39141 %,20167 %,30154 %of the phenotypic variation. They all demonstrated additive effects.Digenic epistasis interactions existed between some detected QTLs related to resistance to Curvularia lunata.
[1] Mandokhot,A M.Survival of Curvularia clavata,incitant of leaf spot of maize[J].Indian Phytopathoiogy,1980,33 (3):396-398.
[2] Macri F.Isolation and characterization of phytotoxins from Curvularia lunata (Wakl';) Boed[J].Physiological Plant Pathology,1976,8 (3):325-331.
[3] Ito M F.Maize leaf spots caused Curvularia lunata Boed[J].Dumma Phytopathologica,1979(3):181-184.
[4] 段双科.玉米自交系黄早4"黄斑病"的调查研究[J].植物保护,1984,10(6):12.
[5] 甘贤友,周围顺,袁桂荣,等.玉米弯孢霉叶斑病初步研究[J].植物保护,1995,21(5):24-25.
[6] 徐德坤,王琪,殷秀东,等.玉米弯孢菌叶斑病调查初报[J].山东农业科学,1995(6):38,50.
[7] 戴法超,高卫东,王晓鸣.玉米弯孢菌叶斑病的初步研究简报[J].植物保护,1996,22(4):36-37.
[8] 吕国忠,陈捷,白金恺,等.我国玉米病害发生现状及防治措施[J].植物保护,1997,23(4):20-21.
[9] 吕国忠,刘志恒,何副刚,等.辽宁省爆发一种新病害-玉米弯孢菌叶斑病[J].沈阳农业大学学报,1997,28(1):75-76.
[10] 戴法超,王晓鸣,朱振东,等.玉米弯孢菌叶斑病研究[J].植物病理学报,1998,28(2):123-129.
[11] 石洁,刘玉瑛,刘爱国,等.河北省玉米弯孢霉菌叶斑病发生调查[J].河北农业大学学报,1998,21(2):94.
[12] 刘玉瑛,石洁,邵艳军.玉米弯孢菌叶斑病接种技术研究[J].华北农学报,1999,14(增刊):96-100.
[13] 刘兰浩,孙阳,穆平,等.部分玉米种质资源对弯孢菌叶斑病的抗性评价[J].玉米科学,2008,16(3):126-129.
[14] 王宏,吕国忠,纪明山,等.玉米杂交种对弯孢菌叶斑病抗性鉴定[J].沈阳农业大学学报,2000,31(5):476-478.
[15] Saghai-Maroof M A,Biyashey R M,Yang G P,et al.Ribosomal DNA spacer length polymorphisms in barley:Mendelian inheritance,chromosomal location and population dynamics[A].Proceeing of the National Academy of Science USA,1984,81:8014-8018.
[16] 黎裕,戴法超,景蕊莲,等.玉米弯孢菌叶斑病抗性的QTL分析[J].中国农业科学,2002,35(10):1221-1227.
[17] 赵君.玉米弯孢菌叶斑病抗性遗传分析及在亲本P138和5003间筛选具有RFLP多态性酶和探针组合[D].北京:中国农业大学,2000.
[18] 黎裕,王天宇,贾继增.玉米抗病虫性的分子标记研究进展[J].生物工程进展,2001,21(3):42-49.
[19] Melchinger A E.Advances in the analysis of data on quantitative trait loci[C] //Intematioonal Crop Science Ⅱ.Oxford IBH Publishing Co,New Delhi,1997:773-791.
[20] 朱军.复杂数量性状基因定位的混合模型方法[C] //王连铮,戴景瑞.全国作物育种学术讨论会论文集.北京:中国农业科技出版社,1998:19-20.
[21] Moreno-Gonzalez J.Efficiency of generations for estimating marker-associated QTL effects by multiple regression[J].Genetics,1993,135:223-231.
[22] 姜丽丽,樊明寿,郭九峰.干旱和灌溉两种处理条件下玉米株高、产量的QTL分析[J].华北农学报,2007,22(4):86-90.
[23] 李玉玲,李学慧,董永彬,等.利用相同来源F2∶3和BC2S1群体定位玉米生育期QTL[J].华北农学报,2007,22(6):38-43.
