论文

来源黑曲霉WP1的两种植酸酶基因的克隆及序列比较

  • 柯晓静 ,
  • 郭润芳 ,
  • 于宏伟 ,
  • 贾英民
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  • 河北农业大学 食品科技学院, 河北 保定 071001
柯晓静( 1980- ), 女, 河北张家口人, 硕士, 主要从事酶工程及食品资源开发研究.

收稿日期: 2008-11-20

  网络出版日期: 2014-10-14

基金资助

河北省"十一五"科技攻关项目资助(06220106D)

Cloning and Sequence Analysis of the phyA and phyB Gene of Aspergillus niger WP1

  • KE Xiao-jing ,
  • GUO Run-fang ,
  • YU Hong-wei ,
  • JIA Ying-min
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  • College of Food Science, Agricultural University of Hebei, Baoding 071001, China

Received date: 2008-11-20

  Online published: 2014-10-14

摘要

为了获得高产植酸酶菌株,利用PCR技术,从黑曲霉WP1中克隆出2种植酸酶基因phyAphyB,分别命名为phyA1phyB1.phyA1长1346 bp,成熟肽序列不含内含子,共编码448个氨基酸;phyB1基因长1560 bp,基因序列含有3段内含子,共编码460个氨基酸.phyA1phyB1序列同源性很低,只有21.12%.二者都含有1个植酸酶基因保守序列RHGXRXP;但phyA1含2个HD保守序列,phyB1只含有1个.将黑曲霉WP1植酸酶phyA1phyB1的基因序列在国际基因库中注册,注册号分别为:DQ60711;DQ836360.

关键词: phyA; phyB1; PCR; 序列同源性

本文引用格式

柯晓静 , 郭润芳 , 于宏伟 , 贾英民 . 来源黑曲霉WP1的两种植酸酶基因的克隆及序列比较[J]. 华北农学报, 2009 , 24(2) : 22 -26 . DOI: 10.7668/hbnxb.2009.02.005

Abstract

In order to get a high phytase activity strain,the phyA1 and phyB1 encoding phytase of Aspergillus niger WP1 were amplified by the polymerase chain reaction(PCR)with primers designed according to the sequences in Gene Bank.The amplified fragments were cloned and sequenced.The results showed that the coding region were 1346 bp and 1560 bp in size,there was no intron in phyA1 gene,encodes a peptide of 448 amino acids;but phyB1 includes 3 introns,and encodes a peptide of 460 amino acid residues.The area of highest homology RHGXRXP was found in the two sequences.There were two HD in phyA1 and only one in phyB1.The phyA1 and phyB1 of A.niger WP1 strain in this paper their sequences have been accessed by Gene Bank(Accession :DQ650711;DQ836360).

Key words: phyB; phyA; PCR; Sequence homology

参考文献

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