长期施用牛粪对松嫩平原盐渍化土壤脱盐碱化效果的影响

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汪勇, 孟庆峰. 长期施用牛粪对松嫩平原盐渍化土壤脱盐碱化效果的影响[J].中国地质调查, 2022,37(2): 142-151.
WANG Yong, MENG Qingfeng. Desalinization Effects of Long-term Cattle Manure Application in Saline-sodic Soils on the Songnen Plain[J].Geological Survey of China, 2022,37(2): 142-151. 复制到剪切板

doi: 10.7668/hbnxb.20192705

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长期施用牛粪对松嫩平原盐渍化土壤脱盐碱化效果的影响

汪勇, 孟庆峰

东北农业大学资源与环境学院,黑龙江哈尔滨 150030

通讯作者:孟庆峰(1982—),男,黑龙江牡丹江人,副教授,博士,主要从事苏打盐碱土壤盐碱障碍消减研究。

作者简介:汪勇(1997—),男,安徽无为人,在读硕士,主要从事苏打盐碱土壤盐碱障碍消减研究。

收稿日期: 2021-12-22

基金资助: 黑龙江省博士后科研启动基金(LBH-Q19073); 东北农业大学“东农学者”(19XG08)

摘要

为了探究施用有机肥对盐碱土壤脱盐效果的长期效应,以腐熟牛粪为改良材料,根据牛粪施用年限共设置4个处理,分别为:施用牛粪8,12,18 a,以未施用牛粪的作为对照。通过对土壤可溶性盐分组成、交换性盐基、有机质、容重、EC和pH值等指标的测定,定量化探讨牛粪对各个指标影响及其关系,阐明长期施用牛粪对盐碱土脱盐效果的影响。结果表明,长期施用牛粪显著降低了土壤HCO₃^-的含量,消除了CO₃^2-;降低了土壤容重,增加了土壤孔隙度和土壤有机质;降低了Na⁺含量,增加了土壤交换性Ca²⁺、可溶性K⁺和Mg²⁺含量。相关性分析表明:土壤ESP和pH值与交换性Na⁺、HCO₃^-和CO₃^2-含量呈显著正相关,土壤pH值与土壤有机质呈显著负相关关系。回归分析表明:影响钠吸附比的主要因素是可溶性Mg²⁺,其次是可溶性Na⁺,即:施用牛粪后,SAR的降低主要是由于可溶性Mg²⁺的增加和可溶性Na⁺的降低而引起的。相关性分析表明:牛粪的累积施用量与土壤pH值、EC、ESP和SAR等呈显著负相关。综上所述,每年施用牛粪导致了土壤pH值、碱化度、电导率和钠吸附比显著降低,这是由于土壤胶体上的Na⁺被游离的Ca²⁺替换,并且促进了土壤可溶性盐从土壤表层淋溶,降低了可溶性盐的含量,也使得可溶性盐的离子组成发生了变化。这些可能是每年施用有机肥导致土壤钙镁离子含量以及土壤孔隙度增加,土壤容重降低的结果。

关键词: 牛粪; 长期试验; 碱土; 交换性阳离子; 可溶性盐; 土壤碱度

中图分类号:S141 文献标志码:A 文章编号:1000-7091(2022)02-0142-10

Desalinization Effects of Long-term Cattle Manure Application in Saline-sodic Soils on the Songnen Plain

WANG Yong, MENG Qingfeng

College of Resources and Environment,Northeast Agricultural University,Harbin 150030,China

Abstract

In order to explore the effects of cattle manure on soil salinity and sodicity on the sodic soil in long-term experiments,the experiments were performed in a randomized complete block design with four treatments;soils that received manure applications for 8,12,18 years were used as the experimental treatments,and soil that did not receive cattle manure application was used as the control treatment(CK).The results showed that the application of cattle manure to saline-sodic soil resulted in a reduction in the bicarbonate ion(HCO₃^-)contents,the elimination of carbonate ions(CO₃^2-),the decrease in soil bulk density(ρ_b),the increases in soil porosity(f_t)and soil organic matter(SOM),the decreases in the exchangeable and soluble sodium ion (Na⁺)contents associated with increases in the exchangeable calcium ion(Ca²⁺),soluble potassium ion(K⁺),and magnesium ion(Mg²⁺)contents compared to those in untreated soil.The soil exchangeable sodium percentage(ESP)and pH were both significantly and positively correlated with the exchangeable Na⁺ and HCO₃^-,and CO₃^2- contents,and soil pH was significantly and negatively correlated with SOM.Regression analysis showed that the dominant factors affecting the sodium absorption ratio(SAR)were the soluble Mg²⁺ and Na⁺ contents in the soil.Pearson correlation analysis showed that there were significantly negative correlation between the accumulated amount of cattle manure among the indicators of soil salinization degree,such as pH,EC,ESP and SAR.It was concluded that long-term manure application significantly decreased the soil pH,ESP,electrical conductivity(EC)and SAR due to the replacement of soil colloidal Na⁺ with Ca²⁺,the leaching of soil soluble salts from the topsoil and changes in the soil soluble salt ion composition.These outcomes were likely due to the decrease of ρ_b associated with increase of f_t and Ca²⁺ and Mg²⁺ contents caused by annual manure application.

Keyword: Cattle manure; Long-term experimentation; Solonetz; Exchangeable cations; Soluble salt; Soil sodicity

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Soil salinization refers to the increase in the soluble salt concentration in water and soils under natural conditions or due to human activities.The parent materials, topographical features, climatic conditions and anthropogenic factors contribute to the formation and evolution of the salinization of the soil^{[1, 2]}.Salt-affected soils with high salinity and alkalinity are not suitable for plant growth and have substantially adversely affected agricultural production and the environment^[3].As a result, soil salinization has become a common environmental problem worldwide^[4].The Songnen Plain in Northeast China is one of the three largest distribution regions of saline-sodic soil in the world; saline-sodic cultivated land accounts for 6.8% of the whole cultivated land area and covers 1.27× 10⁶ ha of the plain^[5].

Saline-sodic soil is typically characterized by high concentrations of sodium(Na⁺), carbonate( ${CO}_{3}^{2 -}$ )and bicarbonate(HCO₃^-)ions on the soil surface that result in high pH, electrical conductivity(EC), sodium adsorption ratio(SAR)and alkalinity values^{[6, 7]}.Among these saline-alkaline components, excessive exchangeable Na⁺ is the predominant factor that results in weaker soil structure, reduce water permeability, and increase osmotic pressure^{[8, 9]}.This suggests that a large area of saline-sodic land being used for agriculture will lead to farming declining or even being abandoned, which will seriously affect agricultural development.

To solve the problems of salinity and sodicity affecting crop production, many techniques have been applied to salt-affected soils, including tillage practices, diversion irrigation, the application of flue gas desulfurization gypsum or organic manure, etc.Organic manure application is a traditional method for the improvement of salt-affected soils in China.Salt-affected soils have been improved and made usable through the application of organic manure to soils.Compared with those in saline-sodic soil without organic amendments, the EC, exchangeable sodium percentage(ESP)and SAR with organic amendments are clearly lower^[10].In addition, Trivedi et al^[11] reported that the application of organic manure helped reduce sodicity factors, such as pH, EC and exchangeable Na⁺, and increased soil fertility.In conclusion, manure application helped improve the physicochemical properties of saline-sodic soils and increased crop yields.However, the results of many of these studies are based on short-term or small-scale experiments, and there are few conclusions from long-term experiments with large-scale organic manure application to soil.Applying organic manure to saline-sodic soil is beneficial for agricultural ecosystems and has the potential to improve the saline-sodic soil.This study aimed to evaluate the continuous effects of long-term cattle manure application on soil salinity and sodicity.Moreover, we also determined how long-term manure application affects the distribution of ions in various soil layers due to the key properties of soils in the study area.

1 Materials and Methods

1.1 Study site

The study was performed on the long-term saline-sodic soil study station located in the Yongle Village of Zhaozhou County, Heilongjiang Province, China(125.06° E, 45.04° N, and altitude 149 m)since 1995.According to the FAO World Soil Resources Reference Base, the soils in this area are classified as solonetz soils, and the soil texture is clay(26.2% sand, 21.5% silt, 52.3% clay)based on the International Society of Soil Science Classification^[12].The climate in this area is a continental monsoon climate in the middle temperate zone, and the area is part of the first accumulated temperature zone.The annual active accumulated temperature above 10 ℃ is approximately 2 800 ℃, the frost-free period is 143 d, the annual average precipitation is 436 mm, the annual average evaporation is 1 800 mm, and the annual average temperature is 3.7 ℃.The soil physicochemical properties prior to experimentation at 0— 20 cm soil depth were illustrated as follows:the soil pH is 9.56; the EC is 6.23 dS/m; the soil bulk density ρ _b is 1.35 g/cm³; and the total porosity(f_t)is 49.06%.

1.2 Experimental design

In late April of each year, cattle manure was applied to soil at a rate of 10 000 kg/ha on an oven-dry weight basis.The cattle manure was equably scattered spread onto the surface of the soil in the experimental area by hand and was immediately incorporated into the surface of the soil at 0— 20 cm by ploughing and ridging before seeding the filed with corn(Zea mays L.).The basic properties of the manure on an oven-dry weight basis were as follows:the pH was 8.42; the SOM was 602.94 g/kg; the total sodium, potassium, calcium and magnesium contents were 0.41, 15.35, 9.77, 4.58 g/kg, respectively; and the bulk density of the manure was 0.29 g/cm³.Based on their history of cattle manure application, five treatments were established in a randomized complete block design with three replicates in late April 2018.The treatments were:a control treatment of corn grown without manure application(CK)and corn grown with manure application for 8(T8), 12(T12), 18 years(T18).Urea(N=46%)was applied as a topdressing at a rate of 400 kg/ha at the jointing stage of corn.

1.3 Soil sampling

To determine the changes in soils as affected by the manure application, soils were sampled from each plot after harvest of the corn in October 2018, three sampling points were randomly selected in each plot, and soil samples from the 0— 20 cm, 20— 40 cm and 40— 60 cm soil layers were used for the determination of the soil salt contents, pH, EC, SOM and exchangeable cation contents, such as exchangeable Na⁺, K⁺, Ca²⁺ and Mg²⁺.Three replicates of soil cores were collected using a cutting ring with a volume of 100 cm³ for the determination of ρ _b and calculation of f_t.

1.4 Laboratory methods

After soil sample collection, the soil samples were treated with the Quartering method to remove any stones and residual crop roots.Each quartered sample weighed approximately 2 kg.After air-drying in a cool and ventilated location, the air-dried soil samples were ground and sieved through a 1.00, 0.25 mm sieve, respectively.

Soil pH was measured at a 2.5:1.0 water-to-soil radio using a pH-meter electrode and EC was measured at a 5:1 water-to-soil radio using a conductivity meter, respectively.The soluble and exchangeable Ca²⁺ and Mg²⁺concentrations were measured by atomic spectrophotometry after extraction with distilled water(5:1 water to soil ratio)and 1 mol/L NH₄OAc, respectively.The soluble and exchangeable K⁺ and Na⁺ concentrations were determined with flame photometry.Soluble ${HCO}_{3}^{-}$ and ${CO}_{3}^{2 -}$ were titrated with double indicator neutralization titration, and silver nitrate was used for the measurements.Soluble chloride(Cl^-)and sulfate( ${SO}_{4}^{2 -}$ )were titrated with EDTA indirect complexometric titration.

The soil cation exchange capacity(CEC)was determined as described by Bao^[13]; after 1 mol/L NaOAc(pH 8.2)saturation of the soil and followed by C₂H₅OH rinsing, Na⁺ was exchanged with 1 mol/L NH₄OAc(pH 7.0)and then determined by flame photometry.

ρ _b was measured using core method by undisturbed soil cores with volume of 100 cm³ and dried for 48 h at 105 ℃^[14], f_t was calculated using ρ _b and particle density(ρ _d)according to the equation ①:

f_t=1- $\frac{ρ_{b}}{ρ_{d}}$ × 100% ①

Where ρ _d is 2.65 g/cm^3[15]; f_t is soil total porosity(%); ρ _b is bulk density(g/cm³); ρ _d is particle density(2.65 g/cm³);

Soil organic matter(SOM)was determined by dichromate oxidation with 230 ℃ heating(K₂Cr₂O₇-H₂SO₄)and back titration with 0.2 mol/L FeSO₄.

ESP and SAR were calculated using equations ② and ③, respectively.

ESP= $\frac{N a_{EXC}^{+}}{CEC}$ × 100% ②

SAR_5:1= $\frac{N a^{+}}{\sqrt[]{\frac{C a^{2 +} + M g^{2 +}}{2}}}$ ③

where ESP is the exchangeable sodium percentage(%); N $a_{EXC}^{+}$ is the exchangeable Na⁺ (cmol/kg); CEC is the cation exchange capacity(cmol/kg); SAR_5:1 is the sodium adsorption ratio(mmol/L) at a 5:1 water to soil ratio; and Na⁺, Ca²⁺and Mg²⁺ are the soluble cations(mmol/L).

1.5 Statistical analysis

All statistical analyses were performed using SPSS 17.0, significant differences among all the measured data were determined with one-way analysis of variance(ANOVA)followed by Duncan test at P< 0.05, all figures were drawn using Origin 2019 and tables were listed using Microsoft Word 2016.

2 Results and Analysis

2.1 Soil pH and EC

The soil pH at 0— 20 cm, 20— 40 cm, 40— 60 cm was obviously affected by manure application and soil pH decreased from 9.35 to 7.92 in topsoil(Fig.1).At 0— 20 cm, the soil pH was significantly(P< 0.05)higher in the CK treatment than in the treatments with manure application, whereas there was no significant difference among the T8, T12 and T18 treatments.Similarly, the highest value of soil pH was observed in the CK treatment compared with other treatments at 20— 40 cm, and the soil pH at 20— 40 cm was significantly higher in the CK treatment than in the other treatments.Although the soil pH changed with the number of years of manure application, no significant difference in soil pH was observed among the T8, T12, and T18 treatments.A similar result was observed at 40— 60 cm, where the highest value of soil pH was also observed in the CK treatment, followed by the T8 and T12 treatments, and the lowest value was observed in the T18 treatment.The soil pH was significantly higher in the CK treatment than in the T8, T12 and T18 treatments.

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	Fig.1 Effects of manure application on soil pH across all treatments Within a soil depth, different letters indicate significant differences using Duncan test at P< 0.05.The same as Fig.2— 5.

At 0— 20 cm, EC decreased by 0.06— 0.39 dS/m and EC was significantly(P< 0.05) lower in the T18 treatment than in the CK treatment.Although the application of manure to the saline-sodic soil resulted in a decrease in EC, there was no significant difference between the CK, T8 and T12 treatments.At 20— 40 cm, the highest value of EC was also observed in the CK treatment compared to other treatments, followed by CK is the T8 and T12 treatments, and the lowest EC value was observed in the T18 treatment.EC was significantly higher in the CK treatment than in the T12 and T18 treatments.EC at 40-60 cm was significantly lower in the manure application treatments than in the CK treatment(Fig.2).

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	Fig.2 Effects of manure application on electrical conductivity(EC) across all treatments

2.2 Soil exchangeable cations and ESP

The soil ESP of the treatments at 0— 20 cm, 20— 40 cm, 40— 60 cm was shown in Fig.3.At 0— 20 cm, soil ESP decreased from 50.01% to 1.54%, and the soil ESP in the T8, T12 and T18 treatment was 43.33, 48.47, 47.79 percentage points lower than that in the CK treatment, respectively.The differences between the manure application treatments and the CK treatment at 0— 20 cm were statistically significant(P< 0.05).Similarly, significant differences were observed at 20— 40 cm between the CK treatment and the treatments with manure application.The manure application treatments showed significantly(P< 0.05) lower ESP than the CK treatment, and there was no significant difference in ESP among the manure application treatments.At 40— 60 cm, the ESP was also significantly higher in the CK treatment than in the T8, T12 and T18 treatments, whereas the differences in ESP were not significant between the T12 and T18 treatments.

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	Fig.3 Effects of manure application on exchangeable sodium percentage(ESP) across all treatments

Tab.1 showed the soil exchangeable cations at different sampling depths, which were influenced by different treatments.The manure application treatments showed significantly lower exchangeable Na⁺than the CK treatment, and significantly higher exchangeable Ca²⁺ and exchangeable Mg²⁺ were observed in the treatments with manure application than CK, except for the exchangeable Mg²⁺ was not significant in the T18 treatment at 0— 20 cm.Exchangeable K⁺ was significantly lower in the CK treatment than in the T8 treatment, whereas there was no significant difference in exchangeable K⁺ among the CK, T12 and T18 treatments at 0— 20 cm.As the exchangeable Ca²⁺ at 20— 40 cm increased, the manure application treatments showed significantly decreased exchangeable Na⁺, by 10.48, 12.19, 12.28 cmol/kg in the T8, T12 and T18 treatments, respectively, relative to the CK treatment.Exchangeable Mg²⁺ and K⁺ were significantly higher in the T8 treatment than in the CK treatment at 20— 40 cm.At 40— 60 cm, the exchangeable Na⁺ was significantly higher in the CK treatment than in the manure application treatments(P< 0.05).A significant difference in exchangeable Ca²⁺ was observed between the CK and treatments with manure application except for the T18 treatment(P< 0.05).In the treatments with manure application, except for the T18 treatment, exchangeable Mg²⁺ showed significantly higher than the CK treatment, whereas no significant difference in exchangeable K⁺ was observed between the CK, T8 and T12 treatments.

Tab.1 Effects of manure application on exchangeable cations across all treatmentscmol/kg

Depths/cm	Treatments	N $a_{Exc}^{+}$	$K_{Exc}^{+}$	C $a_{Exc}^{2 +}$	M $g_{Exc}^{2 +}$
0— 20	CK	12.91± 2.54a	0.67± 0.19c	10.84± 0.97d	7.86± 1.01c
	T8	2.14± 1.04bc	3.16± 0.38a	17.62± 0.81b	14.79± 0.77a
	T12	0.53± 0.04c	0.79± 0.03c	21.61± 0.24a	12.67± 0.10b
	T18	0.67± 0.35c	0.72± 0.04c	20.99± 0.65a	8.16± 0.18c
20— 40	CK	12.75± 2.51a	0.76± 0.09b	10.10± 3.02d	12.37± 1.78b
	T8	2.27± 0.73c	2.38± 0.46a	17.62± 1.34b	14.50± 0.86a
	T12	0.56± 0.03c	0.60± 0.09b	22.39± 0.35a	12.17± 0.13b
	T18	0.47± 0.07c	0.61± 0.07b	16.73± 0.70bc	8.05± 0.38c
40— 60	CK	13.02± 0.26a	0.68± 0.22abc	15.14± 3.57c	7.75± 0.80c
	T8	3.20± 1.18b	0.97± 0.31a	22.35± 2.82ab	14.26± 0.31b
	T12	0.65± 0.08c	0.42± 0.03a	24.91± 1.35a	13.28± 0.76b
	T18	0.52± 0.03c	0.52± .012bc	18.50± 1.15bc	7.35± 0.66c

Note:N $a_{Exc}^{+}$ , $K_{Exc}^{+}$ , C $a_{Exc}^{2 +}$ and M $g_{Exc}^{2 +}$ represents exchangeable sodium ion, calcium ion, potassium ion and magnesium ion, respectively.Mean values± the standard deviation, and different letters indicate significant differences using Duncan test at P< 0.05.The same as Tab.2— 4.

Tab.1 Effects of manure application on exchangeable cations across all treatmentscmol/kg

2.3 Soil soluble cations

The distribution of soluble cations in the different soil layers after manure application was shown in Tab.2.At 0— 20 cm, Na⁺and K⁺ in the CK treatment were significantly higher than those in the treatments with manure application, except for K⁺ in the T8 treatment.The Mg²⁺in the CK treatment was significantly lower than that in the treatments with manure application, except for the T18 treatment, and there was no significant difference in Ca²⁺ between CK and the treatments with manure application, except for the T12 treatment.Similarly, the Na⁺ and K⁺levels decreased significantly with manure application in the 20— 40 cm layer compared with those in the CK treatment.A significant increase in Ca²⁺ in the 20— 40 cm layer was observed in the T12 treatment compared with the CK treatment, whereas there was no significant difference between the CK treatment and the T8 and T18 treatments.There was a significant difference in Mg²⁺ content between the CK, T8 and T12 treatments.Similar results were observed at 40— 60 cm, and Na⁺ and K⁺ in the manure application treatments were significantly different from those in the CK treatment.Although manure application resulted in changes in Ca²⁺ and Mg²⁺ levels in the soil, there was no significant difference in Ca²⁺ and Mg²⁺ levels across all treatments.

Tab.2 Effects of manure application on soluble cations across all treatmentsmg/kg

2.4 Soil soluble anions

The effects of manure application for different time periods on soil soluble anions were shown in Tab.3.The value of ${CO}_{3}^{2 -}$ was high in the CK treatment, whereas it decreased considerably in the manure application treatments such that ${CO}_{3}^{2 -}$ was not measured in the three soil layers.At 0— 20 cm, ${HCO}_{3}^{-}$ and Cl^- were significant higher in the CK treatment than in the treatments with manure application, and there was no significant change in ${SO}_{4}^{2 -}$ between the CK, T8 and T18 treatments.Similarly, ${HCO}_{3}^{-}$ was significantly lower in the manure application treatments than in CK.A change in Cl^-was observed after manure application, but there was no significant difference between treatments with manure application.At 20— 40 cm, the CK treatment had significantly lower ${SO}_{4}^{2 -}$ content than the treatments with manure application, and the ${SO}_{4}^{2 -}$ content gradually decreased with the number of years of manure application.Similar results were observed at 40— 60 cm: ${CO}_{3}^{2 -}$ was found in the CK treatment; ${HCO}_{3}^{-}$ and ${SO}_{4}^{2 -}$ contents were significantly higher in the CK treatment than in the manure application treatments, except S ${O_{4}}^{2 -}$ in the T8 treatment.Although there was a decrease in Cl^- in the manure application treatments, significant differences in Cl^- compared with the CK treatment were observed only in the T12 and T18 treatments.

Tab.3 Effects of manure application on soluble anions across all treatmentsmg/kg

Depths/cm	Treatments	C ${O_{3}}^{2 -}$	HC ${O_{3}}^{-}$	Cl^-	S ${O_{4}}^{2 -}$
0— 20	CK	3.09± 0.18	22.29± 9.28a	8.36± 1.78a	1.26± 0.42b
	T8	—	8.08± 1.75b	5.92± 1.36b	1.74± 0.42b
	T12	—	9.60± 3.50b	4.73± 1.43b	4.50± 1.71a
	T18	—	5.86± 0.35b	5.03± 0.68b	0.62± 0.21b
20— 40	CK	10.44± 6.83	28.04± 16.08a	8.42± 0.75abc	8.64± 2.54a
	T8	—	7.58± 1.52b	9.61± 4.38ab	2.24± 0.32bc
	T12	—	9.19± 0.17b	5.47± 0.51bc	3.58± 0.34b
	T18	—	5.46± 1.05b	3.55± 0.44c	1.16± 0.25c
40— 60	CK	10.44± 6.83	45.17± 13.80a	26.92± 19.87a	14.13± 5.41a
	T8	—	19.71± 10.93b	15.53± 0.89ab	12.07± 1.19a
	T12	—	11.62± 3.16b	3.99± 0.44b	3.50± 0.35b
	T18	—	6.27± 1.26b	3.70± 0.26b	1.56± 0.08b

Tab.3 Effects of manure application on soluble anions across all treatmentsmg/kg

2.5 Soil SAR

The soil SAR was remarkably high in the CK treatment in the three soil layers(Fig.4).At 0— 20 cm, the SAR decreased by 8.05— 9.89 mmol/L and SAR was significantly higher in the CK treatment than in the treatments with manure application, none of the SAR contents in the manure application treatments were higher than 2.33 mmol/L at 0— 20 cm.Although the SAR in the T12 and T18 treatments was lower than that in the T8 treatment, there was no significant difference among those at 0— 20 cm.As at 0— 20 cm, the SAR in the CK treatment in the other soil layers were significantly different from those in the treatments with manure application.Although the application of manure to saline-sodic soil resulted in changes in the SAR, there was no significant difference among the manure application treatments at 20— 40 cm, 40— 60 cm.

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	Fig.4 Effects of manure application sodium adsorption ratio(SAR)across all treatments

2.6 Soil organic matter

The application of manure to saline sodic soil could increase 31.78%— 97.83% SOM content.A similar result was observed in the three soil layers(Fig.5).At 0— 20 cm, the SOM content was significantly lower in the CK treatment than in the T8 and T12 treatments, although the application of manure to the saline sodic soil resulted in an increase in SOM, there was no significant difference between the CK and T18 treatments.Similar result was observed at 20— 40 cm, the SOM content was significantly(P< 0.05)lower in the CK treatment than in the T8 treatment, whereas there was no significant difference among the CK, T12 and T18 treatments.Although the application of manure to saline-sodic soil resulted in changes in the SOM, there was no significant difference among all the application treatments at 40— 60 cm.

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	Fig.5 Effects of manure application soil organic matter(SOM)across all treatments

2.7 Soil bulk density and total porosity

Tab.4 showed the changes of ρ _b and f_t contents at the three layers, which were influenced by cattle manure application.The manure application treatments showed lower ρ _b and higher f_t than the CK treatment and there was a significant increase in ρ _b and decrease in f_t in the T18 treatment compared with the CK treatment at 0— 20 cm.The ρ _b was significantly lower and f_t was significantly higher than those in the CK treatment at 20— 40 cm.There was no significant difference in ρ _b and f_t values across all treatments at 40— 60 cm.

Tab.4 Effects of manure application on soil bulk density and total porosity across all treatments

2.8 Relationships between soil indexes

Relationships between soil indexes were shown in Tab.5.A significantly negative correlation was observed between cumulative amount of manure (CM) and EC by correlation analysis with a correlation coefficient of -0.60(P< 0.01). SAR was significantly and negatively correlated with CM and positively correlated with EC by correlation analysis with correlation coefficients of -0.75 and 0.85, respectively(P< 0.01). ${HCO}_{3}^{-}$ + ${CO}_{3}^{2 -}$ was significantly and negatively correlated with CM and positively correlated with pH by correlation analysis with correlation coefficients of -0.67 and 0.78, respectively(P< 0.01). A significantly negative correlation was observed between SOM and pH by correlation analysis with a correlation coefficient of -0.64(P< 0.01). Exchangeable Na⁺ was significantly and negatively correlated with CM and positively with pH by correlation analysis with correlation coefficients of -0.87 and 0.91, respectively(P< 0.01). A significantly negative correlation was observed between CM and ESP by correlation analysis with a correlation coefficient of -0.84(P< 0.01).

Tab.5 Relationships between soil indexes

Items	CM	pH	EC	SAR	${HCO}_{3}^{-}$ +C ${O_{3}}^{2 -}$	SOM	N $a_{Exc}^{+}$	ESP	ρ _b	f_t
CM	1.00
pH	-0.80^{* *}	1.00
EC	-0.60^{* *}	0.73^{* *}	1.00
SAR	-0.75^{* *}	0.90^{* *}	0.85^{* *}	1.00
${HCO}_{3}^{-}$ + ${CO}_{3}^{2 -}$	-0.67^{* *}	0.78^{* *}	0.33	0.65^{* *}	1.00
SOM	0.27	-0.64^{* *}	-0.35	-0.59^{* *}	-0.32^{* *}	1.00
N $a_{Exc}^{+}$	-0.87^{* *}	0.91^{* *}	0.62^{* *}	0.89^{* *}	0.76^{* *}	-0.58^{* *}	1.00
ESP	-0.84^{* *}	0.91^{* *}	0.57^{* *}	0.84^{* *}	0.79^{* *}	-0.53^{* *}	0.97^{* *}	1.00
ρ _b	-0.76^{* *}	0.66^{* *}	0.61^{* *}	0.71^{* *}	0.08	-0.28	0.71^{* *}	0.65^{* *}	1.00
f_t	0.75^{* *}	-0.65^{* *}	-0.61^{* *}	-0.70^{* *}	-0.06	0.27	-0.70^{* *}	-0.65^{* *}	-1.00^{* *}	1.00

Note:^{* *} means correlation significant at the 0.01 level(two tailed); ^* means correlation significant at the 0.05 level(two tailed); CM means the accumulated amount of cattle manure.

Tab.5 Relationships between soil indexes

3 Discussion and Conclusions

The application of manure to saline-sodic soil significantly increased the SOM compared to untreated soil, which is a direct result of the manure, this is consistent with the results of Zhang et al^[16], who reported that soils treated with manure application significantly increased SOM compared to untreated soil; as well as decreased the ρ _b, particularly at 0— 20 cm depth, decreased ρ _b is associated with increased soil porosity, and the change occurred as a direct result of the addition of the manure, which had a lower bulk density(0.33 g/cm³)than the soil, this result is consistent with Rasool et al^[17], who reported manure application had a positive effect on decrease of ρ _b associated with increase of f_t.In addition, the manure had higher Ca²⁺ and Mg²⁺ contents than the soil, which was beneficial for soil management and reduction of soil salinization^[18].

In our study, treatments in saline-sodic soil with manure application significantly reduced EC content compared to that in the untreated soil.This result was due to annual manure application decreased ρ _b, which is associated with increased f_t that promote the leaching of soil soluble salts from the topsoil by rain^{[19, 20]}.Furthermore, this led to changes in the soil soluble salt ion composition due to differences in migration ability among the soil ions.This result is further confirmed by the soluble Na⁺ in different layers in Tab.2.

The SAR is commonly used as a sensitive indicator of soil alkalization and is usually used to assess the potential for Na⁺ and soil alkalization to affect soil structure^{[21, 22]}.A previous study reported that the application of manure to saline-sodic soil improved the soil structure and that soil soluble salts could be transmitted from the surface soil to the subsoil by soil water; this resulted in a lower soluble Na⁺ content, which led to a reduction in the SAR in soil treated with manure.This is consistent with our results, in our study, the cumulative amount of manure was significantly and negatively related to SAR.In our study, a reduction in the SAR was caused by a decrease in the soil soluble salt content, and the SAR was significantly and positively related to EC.Additionally, the relationships of soluble Na⁺, Ca²⁺ and Mg²⁺ with SAR were determined by linear regression models; the results showed that SAR was significantly and negatively correlated with soluble Mg²⁺ and Ca²⁺ and had a significant correlation with soluble Na⁺ in equation④.This suggested that after manure application to saline-sodic soil, soluble Na⁺ and especially soluble Mg²⁺ were the dominant factors influencing SAR, based on their discriminant coefficients.This probably occurred due to the input of Mg²⁺ from the manure application and the leaching of soluble Na⁺ from the topsoil.This result verifies the findings observed by Yu et al^[23], who discovered that Na⁺ was the predominant soluble cation and that the content of soluble anions was very low at the 0— 50 cm depth on the Songnen Plain before planting.This finding is further confirmed by the contents of soluble cations in Tab.2.

SAR=1.424+9.012Na⁺-20.303Mg²⁺-1.887Ca²⁺ ④

where SAR is the sodium adsorption ratio(mmol/L)and the soluble Na⁺, Ca²⁺ and Mg²⁺ contents in soil are provided in mmol/L.

Soil pH is mainly influenced by the free NaHCO₃ and Na₂CO₃ and exchangeable Na⁺ contents of saline-sodic soil^[24] and their hydrolysis.In our study, the soluble ${HCO}_{3}^{-}$ and ${CO}_{3}^{2 -}$ contents were significantly lower in soil treated with manure than in untreated soil and decreased with the number of years of manure application, soluble ${CO}_{3}^{2 -}$ was eliminated in the soils treated with manure.In our study, the contents of ${HCO}_{3}^{-}$ and ${CO}_{3}^{2 -}$ were significantly and positively related to soil pH.The soil pH was significantly and negatively related to SOM.The decrease in soil pH with manure application is likely due to the organic acid produced during the decomposition of the SOM transformed ${CO}_{3}^{2 -}$ into ${HCO}_{3}^{-}^{[23]}$ , in addition, the input of Ca²⁺ replaced Na⁺ at soil colloid changed into highly soluble salts that restrained the hydrolysis of alkaline Na⁺.Furthermore, the reduction in soil pH is also probably due to the manure application resulted in the decrease of ρ _b, which is associated with increase of f_t that promoted the leaching of soluble Na⁺ from the solution.This result is further confirmed by the correlation among pH, ρ _b and cumulative amount of manure.

The soil exchangeable Na⁺ content decreased with the number of years of manure application.In contrast, the exchangeable Ca²⁺content increased significantly with the decrease in exchangeable Na⁺content in soils treated with manure except for the T18 treatment at 40— 60 cm.This suggested that the exchangeable Ca²⁺replaced the exchangeable Na⁺ in the soil colloids due to the input of Ca²⁺ from manure^[25].In addition, the increase of cumulative amount of manure led to the decrease of ρ _b associated with the increase of f_t, respectively promoted the leaching of soluble Na⁺ from the solution and then reducing the hydrolysis of alkaline Na⁺ as a result of decrease in soil pH.Thus, the decrease in soil pH was caused by a reduction in the exchangeable Na⁺ content; soil pH was significantly and positively correlated with exchangeable Na⁺.A similar result was observed by Meng et al^[10] in a laboratory soil column leaching experiment; they reported that amended soils had significantly higher soil exchangeable Ca²⁺ levels than control soils and that soil exchangeable Na⁺ concentrations were significantly lower in organic amendment-treated soils than in control soils after leaching.

Soil ESP is expressed as the saturation of exchangeable Na⁺ adsorbed on soil colloids.In general, the higher the ESP is, the higher the degree of soil alkalization and the poorer the soil properties.A reduction in soil ESP reflects a low level of exchangeable Na⁺ relative to the levels of other exchangeable cations^[18].In our study, soil ESP was significantly lower in the treated soils than in the untreated soil.This was likely due to a decrease in the exchangeable Na⁺ content and the leaching of soluble ions from the solution in topsoil as a result of increase of f_t, which affected by the manure application between cumulative amount of manure and ESP.In addition, soil ESP decreased significantly with decreasing exchangeable Na⁺ content.These findings are also consistent with the results presented by Mao et al^[26], who reported that an increase in soluble Ca²⁺ in the soil resulted in a reduction in the amount of exchangeable Na⁺ present in soil colloids.

The experiments were carried out by applying cattle manure application on sodic soil on the Songnen Plain, Northeast China, we studied the desalinization effects of long-term cattle manure application in saline-sodic soils and the results showed that the application of cattle manure to saline-sodic soil resulted in an increase in SOM, a reduction in pH, EC, ESP and SAR, compared with CK treatment at 0— 20 cm, SOM increased by 31.78%— 97.83%; pH and ESP decreased from 9.35 to 7.92 and from 50.01% to 1.54%, EC and SAR decreased by 0.06— 0.39 dS/m and 8.05— 9.89 mmol/L, respectively.The application of cattle manure to saline-sodic soil resulted in a change the ion composition.The application of cattle manure to saline-sodic soil resulted in an elimination of ${CO}_{3}^{2 -}$ , decreases in exchangeable and soluble Na⁺ contents associated with increases in exchangeable Ca²⁺, a decrease in soluble K⁺ and an increase in soluble Mg²⁺ contents, compared to those in untreated soil.The higher the accumulated amount of cattle manure, the more obvious the desalination effect in the treatments.There was an extremely significant negative correlation between the accumulated amount among the indicators of soil salinization degree, such as pH, EC, ESP and SAR.We concluded that the observed decreases in soil pH and ESP were caused by the replacement of soil colloidal Na⁺ with Ca²⁺.Moreover, the reductions in soil salinity and sodicity(soil pH, EC and SAR)resulted from the leaching of soil soluble salts from the topsoil and changes in the soil soluble salt ion composition.This result was likely due to the decrease in ρ _b associated with increase in f_t and the increased Ca²⁺and Mg²⁺contents caused by annual manure application.

参考文献

文献选项

[1]	He K, Li X P, Dong L L. The effects of flue gas desulfurization gypsum(FGD gypsum)on P fractions in a coastal plain soil[J]. Journal of Soils and Sediments, 2018, 18(3): 804-815. doi: 10.1007/s11368-017-1821-2. [本文引用:1]
[2]	Wei L X, Lü B S, Li X W, Wang M M, Ma H Y, Yang H Y, Yang R F, Piao Z Z, Wang Z H, Lou J H, Jiang C J, Liang Z W. *Priming of rice( Oryza sativa* L. )seedlings with abscisic acid enhances seedling survival, plant growth, and grain yield in saline-alkaline paddy fields*[J]. Field Crops Research*, 2017, 203: 86-93. doi: 10.1016/j.fcr.2016.12.024. [本文引用:1]
[3]	支晓蓉, 杨秀艳, 任坚毅, 武海雯, 朱建峰, 张华新. 我国园林植物耐盐性评价及鉴定研究进展[J]. 世界林业研究, 2018, 31(5): 51-57. doi: 1013348/j. cnki. sjlyyj. 2018. 0073. y. Zhi X R, Yang X Y, Ren J Y, Wu H W, Zhu J F, Zhang H X. Research progress in salt tolerance evaluation and identification of garden plants in China[J]. World Forestry Research, 2018, 31(5): 51-57. [本文引用:1]
[4]	王遵清. 中国盐碱土[M]. 北京: 科学出版社, 1993 Wang Z Q. Salt-affected soils in China[M]. Beijing: Science Press, 1993. [本文引用:1]
[5]	姚荣江, 杨劲松, 刘广明. 东北地区盐碱土特征及其农业生物治理[J]. 土壤, 2006, 38(3): 256-262. doi: 103321/j. issn: 0253-9829. 2006. 03. 004. Yao R J, Yang J S, Liu G M. Characteristics and agro-biological management of saline-alkalized land in Northeast China[J]. Soils, 2006, 38(3): 256-262. [本文引用:1]
[6]	李彬, 王志春, 迟春明. 吉林省大安市苏打碱土含盐量与电导率的关系[J]. 干旱地区农业研究, 2006, 24(4): 158-171. doi: 101016/S1872-2040(06)60047-9. Li B, Wang Z C, Chi C M. The relationship between salt content and electric conductivity of soda solonetz in Da'an City[J]. Agricultural Research in the Arid Area, 2006, 24(4): 168-171. [本文引用:1]
[7]	Li X B, Kang Y H, Wang X M. Response of soil properties and vegetation to reclamation period using drip irrigation in coastal saline soils of the Bohai gulf[J]. Paddy and Water Environment, 2019, 17(4): 803-812. doi: 10.1007/s10333-019-00758-7. [本文引用:1]
[8]	Zhao X M, Zhu M L, Guo X X, Wang H B, Sui B, Zhao L P. Organic carbon content and humus composition after application aluminum sulfate and rice straw to soda saline-alkaline soil[J]. Environmental Science and Pollution Research International, 2019, 26(14): 13746-13754. doi: 10.1007/s11356-018-2270-1. [本文引用:1]
[9]	王春裕, 武志杰, 石元亮, 王汝镛. 中国东北地区的盐渍土资源[J]. 土壤通报, 2004, 35(5): 643-647. doi: 1019336/j. cnki. trtb. 2004. 05. 028. Wang C Y, Wu Z J, Shi Y L, Wang R Y. The resource of saline soil in the northeast China[J]. Chinese Journal of Soil Science, 2004, 35(5): 643-647. [本文引用:1]
[10]	Meng Q F, Ma X F, Zhang J, Yu Z T. The long-term effects of cattle manure application to agricultural soils as a natural-based solution to combat salinization[J]. Catena, 2019, 175: 193-202. doi: 10.1016/j.catena.2018.12.022. [本文引用:2]
[11]	Trivedi P, Singh K, Pankaj U, Verma S K, Verma R K, Patra D D. Effect of organic amendments and microbial application on sodic soil properties and growth of an aromatic crop[J]. Ecological Engineering, 2017, 102: 127-136. doi: 10.1016/j.ecoleng.2017.01.046. [本文引用:1]
[12]	IUSS Working Group W R B. World reference base for soil resources 2006(2nd ed)[S]. World Soil Resources Report NO. 103. FAO, Rome, 2006. [本文引用:1]
[13]	鲍士旦. 土壤农化分析[M]. 北京: 中国农业出版社, 1999. Bao S D. Soil and agricultural chemistry analysis[M]. Beijing: China Agriculture Press, 1999. [本文引用:1]
[14]	Wang R Z. Soil particle density, bulk density and porosity determination;soil particle analyses;soil moisture determination[EB/OL]. 1989 [本文引用:1]
[15]	Alhaj Hamoud Y, Guo X P, Wang Z C, Chen S, Rasool G. Effects of irrigation water regime, soil clay content and their combination on growth, yield, and water use efficiency of rice grown in South China[J]. International Journal of Agricultural and Biological Engineering, 2018, 11(4): 126-136. doi: 10.25165/j.ijabe.20181104.3895. [本文引用:1]
[16]	Zhang Y, E S Z, Wang Y N, Su S M, Bai L Y, Wu C X, Zeng X B. Long-term manure application enhances the stability of aggregates and aggregate-associated carbon by regulating soil physicochemical characteristics[J]. Catena, 2021, 203: 105342. doi: 10.1016/j.catena.2021.105342. [本文引用:1]
[17]	Rasool R, Kukal S S, Hira G S. Soil organic carbon and physical properties as affected by long-term application of FYM and inorganic fertilizers in maize-wheat system[J]. Soil and Tillage Research, 2008, 101(1/2): 31-36. doi: 10.1016/j.still.2008.05.015. [本文引用:1]
[18]	Zhao Y G, Wang S J, Li Y, Liu J, Zhuo Y Q, Chen H X, Wang J, Xu L Z, Sun Z T. Extensive reclamation of saline-sodic soils with flue gas desulfurization gypsum on the Songnen Plain, Northeast China[J]. Geoderma, 2018, 321: 52-60. doi: 10.1016/j.geoderma.2018.01.033. [本文引用:2]
[19]	刘哲, 孙增慧, 吕贻忠. 长期不同施肥方式对华北地区温室和农田土壤团聚体形成特征的影响[J]. 中国生态农业学报, 2017, 25(8): 1119-1128. doi: 1013930/j. cnki. cjea. 161060. Liu Z, Sun Z H, Lü Y Z. Effect of long-term fertilization on soil aggregate formation in greenhouse and farmland conditions in the North China Plain[J]. Chinese Journal of Eco-Agriculture, 2017, 25(8): 1119-1128. [本文引用:1]
[20]	Sharma P K, Verma T S, Bhagat R M. *Soil structural improvements with the addition of Lantana camara* biomass in rice-wheat cropping*[J]. Soil Use and Management*, 1995, 11(4): 199-203. doi: 10.1111/j.1475-2743.1995.tb00956.x. [本文引用:1]
[21]	张济世, 于波涛, 张金凤, 刘玉明, 蒋曦龙, 崔振岭. 不同改良剂对滨海盐渍土土壤理化性质和小麦生长的影响[J]. 植物营养与肥料学报, 2017, 23(3): 704-711. doi: 1011674/zwyf. 16415. Zhang J S, Yu B T, Zhang J F, Liu Y M, Jiang X L, Cui Z L. Effects of different amendments on soil physical and chemical properties and wheat growth in a coastal saline soil[J]. Journal of Plant Nutrition and Fertilizers, 2017, 23(3): 704-711. [本文引用:1]
[22]	Luo J M, Yang F, Wang Y J, Ya Y J, Deng W, Zhang X P, Liu Z. Mechanism of soil sodification at the local scale in Songnen plain, Northeast China, as affected by shallow groundwater table[J]. Arid Land Research and Management, 2011, 25(3): 234-256. doi: 10.1080/15324982.2011.565856. [本文引用:1]
[23]	Yu P J, Liu S W, Yang H T, Fan G H, Zhou D W. Short-term land use conversions influence the profile distribution of soil salinity and sodicity in Northeastern China[J]. Ecological Indicators, 2018, 88: 79-87. doi: 10.1016/j.ecolind.2018.01.017. [本文引用:1]
[24]	Mashhady A S, Rowell D L. Soil alkalinity. i. equilibria and alkalinity development[J]. Journal of Soil Science, 1978, 29(1): 65-75. doi: 10.1111/j.1365-2389.1978.tb02032.x. [本文引用:1]
[25]	Sakai Y J, Matsumoto S, Sadakata M. Alkali soil reclamation with flue gas desulfurization gypsum in China and assessment of metal content in corn grains[J]. Soil and Sediment Contamination: an International Journal, 2004, 13(1): 65-80. doi: 10.1080/10588330490269840. [本文引用:1]
[26]	Mao Y M, Li X P, Dick W A, Chen L M. Remediation of saline-sodic soil with flue gas desulfurization gypsum in a reclaimed tidal flat of southeast China[J]. Journal of Environmental Sciences, 2016, 45: 224-232. doi: 10.1016/j.jes.2016.01.006. [本文引用:1]

2018

0.0

... The parent materials,topographical features,climatic conditions and anthropogenic factors contribute to the formation and evolution of the salinization of the soil^[1,2] ...

2017

0.0

... The parent materials,topographical features,climatic conditions and anthropogenic factors contribute to the formation and evolution of the salinization of the soil^[1,2] ...

2018

0.0

... Salt-affected soils with high salinity and alkalinity are not suitable for plant growth and have substantially adversely affected agricultural production and the environment^[3] ...

1993

0.0

... As a result,soil salinization has become a common environmental problem worldwide^[4] ...

2006

0.0

... 10⁶ ha of the plain^[5] ...

2006

0.0

... Saline-sodic soil is typically characterized by high concentrations of sodium(Na⁺),carbonate(C O32-)and bicarbonate(HCO₃^-)ions on the soil surface that result in high pH,electrical conductivity(EC),sodium adsorption ratio(SAR)and alkalinity values^[6,7] ...

2019

0.0

2019

0.0

... Among these saline-alkaline components,excessive exchangeable Na⁺ is the predominant factor that results in weaker soil structure,reduce water permeability,and increase osmotic pressure^[8,9] ...

2004

0.0

2019

0.0

... Compared with those in saline-sodic soil without organic amendments,the EC,exchangeable sodium percentage(ESP)and SAR with organic amendments are clearly lower^[10] ...

... A similar result was observed by Meng et al^[10] in a laboratory soil column leaching experiment ...

2017

0.0

... In addition,Trivedi et al^[11] reported that the application of organic manure helped reduce sodicity factors,such as pH,EC and exchangeable Na⁺,and increased soil fertility ...

2006

0.0

... 3% clay)based on the International Society of Soil Science Classification^[12] ...

1999

0.0

... The soil cation exchange capacity(CEC)was determined as described by Bao^[13] ...

1989

0.0

... _b was measured using core method by undisturbed soil cores with volume of 100 cm³ and dried for 48 h at 105 ℃^[14],f_t was calculated using #cod#x003c1 ...

2018

0.0

... 65 g/cm^3[15] ...

2021

0.0

... 3 Discussion and ConclusionsThe application of manure to saline-sodic soil significantly increased the SOM compared to untreated soil,which is a direct result of the manure,this is consistent with the results of Zhang et al^[16],who reported that soils treated with manure application significantly increased SOM compared to untreated soil ...

2008

0.0

... 33 g/cm³)than the soil,this result is consistent with Rasool et al^[17],who reported manure application had a positive effect on decrease of #cod#x003c1 ...

2018

0.0

... In addition,the manure had higher Ca²⁺ and Mg²⁺ contents than the soil,which was beneficial for soil management and reduction of soil salinization^[18] ...

... A reduction in soil ESP reflects a low level of exchangeable Na⁺ relative to the levels of other exchangeable cations^[18] ...

2017

0.0

... _b,which is associated with increased f_t that promote the leaching of soil soluble salts from the topsoil by rain^[19,20] ...

1995

0.0

... _b,which is associated with increased f_t that promote the leaching of soil soluble salts from the topsoil by rain^[19,20] ...

2017

0.0

... The SAR is commonly used as a sensitive indicator of soil alkalization and is usually used to assess the potential for Na⁺ and soil alkalization to affect soil structure^[21,22] ...

2011

0.0

... The SAR is commonly used as a sensitive indicator of soil alkalization and is usually used to assess the potential for Na⁺ and soil alkalization to affect soil structure^[21,22] ...

2018

0.0

... This result verifies the findings observed by Yu et al^[23],who discovered that Na⁺ was the predominant soluble cation and that the content of soluble anions was very low at the 0#cod#x02014 ...

1978

0.0

... Soil pH is mainly influenced by the free NaHCO₃ and Na₂CO₃ and exchangeable Na⁺ contents of saline-sodic soil^[24] and their hydrolysis ...

2004

0.0

... This suggested that the exchangeable Ca²⁺replaced the exchangeable Na⁺ in the soil colloids due to the input of Ca²⁺ from manure^[25] ...

2016

0.0

... These findings are also consistent with the results presented by Mao et al^[26],who reported that an increase in soluble Ca²⁺ in the soil resulted in a reduction in the amount of exchangeable Na⁺ present in soil colloids ...