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2019 Vol. 34, No. 2
Published: 2019-04-28

Crop Genetics·Germplasm Resources·Biotechnology
Tillage and Cultivation·Physiology and Biochemistry
Environment·Plant Protection
0 Table of Contents for VOL.34 No.2
2019 Vol. 34 (2): 0-0 [Abstract] ( 161 ) 全文: HTML   PDF (244 KB)  ( 47 )
       Crop Genetics·Germplasm Resources·Biotechnology
1 Molecular Characterization and Target Gene Prediction of Plant miR399 Family
LIU Chao, CHU Honglong, HAN Lihong, DAI Dongqin, CHEN Huanhuan, TANG Lizhou
DOI: 10.7668/hbnxb.201751113
MicroRNA plays a variety of important regulatory roles in cells. The miR399 family plays a role in plant response to phosphorus starvation stress. In order to further understand the composition, molecular characteristics and target gene function of plant miR399 family, the number, phylogeny, secondary structure, promoter characteristics and target genes of miR399 family were analyzed by bioinformatics methods. Results showed that a total of 244 mature miR399 members were obtained, distributing only in angiosperms. The distribution of pre-miR399 and mature miR399 in different species varied greatly. Most pre-miR399 was only cut into a single mature miR399. The pre-miR399 with the largest number was 64 nt, and 93.85% of mature miR399 was only 21 nt. Pre-miR399 sequence analysis showed that 3' end was more conservative than 5' end. Most of the mature miR399 sequence was derived from the 3' end of pre-miR399, and the conservative sequence was UGCCAAAGGAGA*UUGCCC*G. Evolutionary analysis showed that the miR399 sequence was more consistent, but the cluster relationship of different species was complex. pre-miR399 was mainly divided into three types:①Producing one mature miR399 from 3' end;②Producing two mature miR399 respectively from 3' and 5' end; ③Producing one mature miR399 from 5' end. The pre-miR399 promoter region contained a large number of hormones and stress response elements. Gene ontology analysis of the target genes in rice miR399 family showed that the proportion of the cell and the cell part classifications were 59.1%, while the proportion of the catalytic activity and the binding types were 40.9% and 45.5% respectively. The miR399 regulated multiple phosphorus response and phosphorus transport target genes. Those target genes are mainly involved in plant phosphorus metabolism, stress response, gene expression regulation and many other processes. Above results indicated that miR399 was highly conserved and participated many signal processes, and played a role in plant phosphorus starvation stress and phosphorus transport.
2019 Vol. 34 (2): 1-7 [Abstract] ( 118 ) 全文: HTML   PDF (1182 KB)  ( 115 )
8 Cloning and Expression of ZmPP2C6-01 Gene in Maize
YUAN Zhen, ZHANG Pengyu, WANG Guorui, CAO Liru, KU Lixia, WEI Li
DOI: 10.7668/hbnxb.201751477
In order to study the response of maize serine/threonine protein phosphatase PP2C gene to abiotic stress and its physiological function, the transcriptome of maize inbred line Yu 882 was analyzed under 20% PEG6000 stress. The PP2C gene strongly expressed under drought stress was screened and cloned, while the bioinformatics analysis and expression pattern analysis were carried out. A PP2C protein gene that was up-regulated significantly by drought stress was found in the 20% PEG6000 stress-treated transcriptome of maize, and was named as ZmPP2C6-01. The open reading frame of ZmPP2C6-01 gene was 1 242 bp, encoding 413 amino acids. The encoded protein had a molecular mass of 43.8 ku and an isoelectric point of 6.6. It was a hydrophilic protein. The ZmPP2C6-01 protein was closely related to the PP2C protein of sorghum, and also had the close relationship with the PP2C protein of winter wheat and Aegilops tauschii. The ZmPP2C6-01 protein was localized in the nucleus. Real-time quantitative PCR analysis showed that ZmPP2C6-01 gene had the highest expression in maize roots, followed by leaves and stems. Under the condition of PEG stress, the expression level of ZmPP2C6-01 gene in roots was higher than that of the control in most of the time, and it was up-regulated in leaves. However, under the stresses of ABA, NaCl and high temperature, the expression level of ZmPP2C6-01 gene in roots was lower than that of the control, and the overall expression showed a downward trend, while the expression level in leaves was higher than that of the control, showing an up-regulated expression pattern. In summary, the ZmPP2C6-01 gene responded to drought and other stresses, but the expression patterns were inconsistent.
2019 Vol. 34 (2): 8-18 [Abstract] ( 82 ) 全文: HTML   PDF (1445 KB)  ( 52 )
19 Cloning and Expression of LRR-RLK Gene LP7 in Rice
LIN Faming, LI Shen, WANG Ke, GAO Junfeng, LI Guanghao, WANG Daichang, DU Changqing, ZHAO Quanzhi
DOI: 10.7668/hbnxb.201751355
LRR-RLK (Leucine-rich repeat receptor-like protein kinase) is the largest subfamily of the receptor-like protein kinase RLK family and plays an important role in regulating the plant abiotic stress. To understand the role of rice LRR-RLK member LP7 (LOC_Os05g24010) in resistance to low phosphorus stress, the full-length sequence of LP7 was cloned from rice variety Nipponbare, and its deduced amino acid sequence was analyzed to study the tissue expression pattern, subcellular localization and expression change under low phosphorus stress. The results showed that the LP7 gene was 2 832 bp in length and encoded 943 amino acids. The LP7 protein had the typical characteristics of LRR-RLK membership. The LP7 protein had high homology with the homologous protein NP_001131018 of maize, reaching to 77%. Expression patterns showed that LP7 could express in roots, stems, leaves and other tissues, but the highest in leaves. Subcellular localization result indicated that the LP7 protein was localized on the cell membrane. Real-time quantitative PCR analysis showed that the LP7 gene was induced by low-phosphorus stress, and its expression level increased by 15 times, compared to normal culture condition. The results indicated that LP7 might have important functions in response to low phosphorus stress in rice.
2019 Vol. 34 (2): 19-24 [Abstract] ( 80 ) 全文: HTML   PDF (1246 KB)  ( 72 )
25 Effects of GA3 and PBZ on Shoot Growth and Expression of GA-related Genes in Peach
TAN Bin, WANG Ting, HAO Pengbo, ZHENG Xianbo, CHENG Jun, WANG Wei, FENG Jiancan
DOI: 10.7668/hbnxb.201751523
To investigate the effects of exogenous GA3 and PBZ treatments on the growth of shoots and the expression of genes related to gibberellin biosynthesis and gibberellin signal transduction pathway in Huangshuimi, the annual shoots of Huangshuimi were treated with exogenous GA3 and PBZ. The growth of shoots was measured and the expression levels of eight GA-related genes were determined by qRT-PCR. The results showed that the net length of shoots at 8 d was significantly higher in GA3 treatment than in CK. The expressions of KO and GA3-ox showed a trend of firstly increasing and then decreasing in the early stage after treatment, while gradually rising in the later stages. The expression of DELLA, GID1c and SLY1 genes related to GA signal transduction was inhibited at the early stage and then rapidly increased in the late growth period after treatment. And the expression of the three genes was up to maximum in 14 d after treatment. For the PBZ treatment, the net length of shoots was lower than the CK at many stages, and reached to a significant level at 17 d after treatment. The expression of genes related to gibberellin biosynthesis KO, GA20-ox and GA2-ox showed the trend of increasing gradually and then decreased. While GA3-ox showed almost no expression in whole treatment period. The expression of DELLA, GID1c, SLY1 and ERF11 related to gibberellin signal transduction pathway were slightly lower than the CK during the whole process. It was speculated that the exogenous PBZ might regulate the plant growth at the transcriptional level by inhibiting the synthesis of active gibberellin and further affecting the expression of genes involved in the gibberellin signal transduction pathway. Meanwhile, the reverse treatment of GA3 could significantly abolish the inhibitory effect of PBZ on plant growth, and the expression of GID1c gene involved in gibberellin signal transduction pathway increased firstly and then decreased under GA3 treatment, while the expression of DELLA gene appeared to be inhibited in most of the time.
2019 Vol. 34 (2): 25-34 [Abstract] ( 48 ) 全文: HTML   PDF (1266 KB)  ( 72 )
35 Genome-wide Identification and Analysis of Gene Families Related to Fruit Ripening of Melon
GUO Chengyu, LIU Jun, LI Yuanlei, AN Rui, LI Xingyan, HASI Agula
DOI: 10.7668/hbnxb.201750903
To obtain the genes related to fruit maturation of melon, the genome-wide identification of four gene families, HB(Homeobox),RIN(Ripening inhibitor),ETR(Ethylene receptor) and CTR(Constitutive triple reaction) from melon, which were related to ethylene biosynthesis and signal transduction, were carried out in the study. A total of 17 members of CmHB family,21 members of CmRIN family,3 members of CmETR family and 20 members of CmCTRs family were retrieved from the melon genome, which were further verified by sequence alignment and motif analysis. Using transcriptional profiling analysis, the expressions of the four gene family members in wild type Cucumis melo Hetao and transgenic Cucumis melo Hetao with antisense CmACO1 gene (M9) were determined during growth period and at ripening stage of fruits. The results showed that 13 genes were differentially expressed between the two developmental stages of fruit in wild type melon, of which the expression amount of CmHB3 and CmHB11 in growth period was, respectively, 42 and 9 times that at ripening stage, but the expression level of CmHB4 at ripening stage was 27 times that in growth period, all reaching to a highly significant level. And 12 genes were also differentially expressed between two developmental stages of fruit in M9 transgenic line, among which the expression amount of CmHB3 and CmHB11 in growth period was, respectively, 6 and 3 times that at ripening stage, while the expression level of CmHB4 at ripening stage was 41 times that in growth period, all the differences being highly significant. During the growth of fruit, the expression levels of CmHB3 and CmHB11 had extremely significant difference, and the expression levels of CmRIN14 and CmRIN15 were significantly different between wild type and M9 fruits. At ripening stage of fruit, the difference in expression level of CmHB3 was highly significant and the differences in expression levels of CmRIN14 and CmRIN15 were significant between two types of melon fruits. In addition, the expression patterns of CmRIN14 and CmRIN15 between the two melon materials presented a opposite trend,indicating that their expression patterns were affected by the expression level of CmACO1.
2019 Vol. 34 (2): 35-43 [Abstract] ( 62 ) 全文: HTML   PDF (1652 KB)  ( 58 )
44 Genome-wide Identification and Expression Analysis of bZIP Transcription Factor Family in Jatropha curcas
WANG Haibo, GUO Junyun, TANG Lizhou, LIU Chao
DOI: 10.7668/hbnxb.201750960
The purpose of the study was to further understand the structure characteristics of Basic leucine zipper (bZIPs) and its function in response to biotic and abiotic stress. Based on the Jatropha curcas genome and bioinformatics methods, bZIP family genes from J.curcas were identified, and then the gene structure, phylogentic relationship, chromosomal location, synteny relationship, as well as organ and chilling hardening expression, were systematically analyzed. The results showed that a total of 51 bZIP genes were systematically identified from J.curcas genome and classified into 10 subfamilies (A-I and S) according to phylogentic relationship. Chromosome mapping analysis indicated that J.curcas bZIP genes were distributed with different densities on 11 chromosomes, and tandem duplications were found on chromosome No.2 and 4, which was the main power for the expansion of J.curcas bZIP gene family. The results of gene structure analysis revealed that most of the J.curcas bZIP gene contained 1-13 exons, and the member of subfamilies of S and G owned 1-2 and 12-13 exons, respectively. Subcellular localization showed that the predicted bZIP proteins mainly located in nucleus with amino acid number ranged from 113 to 768 aa and pI value distributed from 4.70 to 10.30. 3-27 hormonal response elements for gibberellin, abscisic acid, ethylene, auxin and abiotic stress response elements like low temperature, heat and wound were identified in the promoter of J.curcas bZIP genes. Transcriptome expression analysis showed that J.curcas bZIP genes exhibited different tissue-specific expression patterns, and 26 J.curcas bZIP genes were found to be expressed in the detected organs of leaves, roots, and seeds, while the others were expressed in the specific organ tissues. Moreover, the expression profiling of 14 J.curcas bZIP genes were up-regulated under chilling hardening. In leaves, JcbZIP3 and JcbZIP14 reached to the highest expression levels after 24 h chilling hardening based on the qRT-PCR results, which were associated with the events of chilling resistance formation and cold signalling transduction. These results were helpful for the cloning and regulation mechanism analysis of bZIP genes in J.curcas.
2019 Vol. 34 (2): 44-58 [Abstract] ( 48 ) 全文: HTML   PDF (2031 KB)  ( 13 )
59 Functional Analysis of A7-FT Promoter in Brassica napus L.
ZHANG Xiyu, HE Hui, LI Zhen, XING Man, HONG Bo, GUAN Chunyun
DOI: 10.7668/hbnxb.201751081
Based on the whole genome sequence of Brassica napus, the sequence of A7-FT gene promoter was obtained by PCR. The promoter online prediction software was used to predict the function and structure of the promotor, and five promoter sequences with different fragment lengths were obtained from the 5'-end deletion according to the distribution of predicted cis-elements. The GUS gene expression vector containing promoters with different fragment lengths was constructed and T2 seedlings were obtained by Agrobacterium -mediated Arabidopsis thaliana. After GUS staining and decolorization, the function of A7-FT gene promoter was explored to study the flowering of B. napus. The study of regulatory mechanisms provided a theoretical basis.The A7-FT gene promoter sequence was obtained from the DNA of Brassica napus L. Xiangyou 15 by PCR. Using the PLACE and PlantCARE online tools to predict the sequence, it was found that in addition to the promoter core elements CAATbox and TATAbox,the A7-FT gene promoter also had photo responses, hormone response components, expression response element of endosperm,response element of stress resistance and physiological control. Based on the predicted distribution of cis-acting elements, specific primers were designed to clone the promoter with different fragment lengths. A 5' segment deletion vector was constructed with pCAMBIA1303 vector and designated as M1, M2, M3, M4, and M5, respectively. Through Agrobacterium -mediated Arabidopsis thaliana, GUS staining and decolorization results showed that there might be some negative regulatory element binding sites between-1 549 and-238, and the core region of the promoter was from-238 to +1.
2019 Vol. 34 (2): 59-65 [Abstract] ( 33 ) 全文: HTML   PDF (1298 KB)  ( 16 )
66 Cloning and Expression Characteristics of Solanum tuberosum StPR1 Gene
LI Xiuyu, HE Fumeng, HAN Yingqi, ZHAO Xiaocan, WU Jiawen, ZHU Yuanfang, ZHOU Lei, SHI Qihai, FENG Zhe, LI Fenglan
DOI: 10.7668/hbnxb.201751433
In order to explore the correlation between pathogenesis related protein (PR protein) and potato disease resistance, the ability to resist soft rot, bacterial wilt and dry rot in potato was tested. In the previous potato disease resistance study, 17 family gene-binding transcriptome data of PRs were analyzed and the genes were screened, and PR1 was finally determined. The potato Atlantic variety was selected as the material, which was provided by Heilongjiang Academy of Agricultural Sciences. The StPR1 gene was cloned and analyzed by bioinformatics. F.sambucinum and F.avenaceum were used. And E.carotovora subsp. Carotovora Borgey (Ecc), E.chrysanthemi Burkholder. Atroseptica Dye (Ech), can cause soft rot and bacterial wilt. The specific analysis of StPR1 gene expression was carried out after inoculation of potato tuber with E. carotovora subsp. Mc Fadden et Dimock (Eca) and Ralstonia solanacearum (RS). The results showed that the StPR1 gene was 540 bp in length and encoded 179 amino acids. The hydrophobicity and hydrophilicity of the protein are predicted.The GRAV value of the PR1 protein sequence was between +4 and-3, containing hydrophilic and hydrophobic regions, and protein secondary structure. It is predicted to be a mixed protein. The tertiary structure of the protein is predicted to be a tightly complex helical structure with a SCP_PR-1_like conserved domain, which is highly similar to the pepper PR1 gene on an evolutionary branch. The results of qRT-PCR showed that the expression level of StPR1 gene was higher than that of bacterial stress in antifungal stress, and its ability to resist dry rot was better than that against soft rot and bacterial wilt. In summary, when the potato is attacked by external pathogens, StPR1 plays an important role in the disease resistance of the potato.In summary, when the potato is attacked by external pathogens, StPR1 plays an important role in the disease resistance of the potato.
2019 Vol. 34 (2): 66-71 [Abstract] ( 29 ) 全文: HTML   PDF (1092 KB)  ( 18 )
72 Identification and Expression Analysis of Whirly Gene Family in Capsicum annuum L.
LÜ Yanyan, WEI Xiaochun, ZHAO Yanyan, YUAN Yuxiang, WANG Zhiyong, YANG Shuangjuan, ZHENG Xiaolan, JIANG Jun, LI Yan, ZHANG Mengyuan, YAO Qiuju, ZHANG Qiang, ZHANG Xiaowei
DOI: 10.7668/hbnxb.201750777
To preliminarily explore the functions and evolutionary relations of Whirly gene family members, the bioinformatics was used to analyze the gene structure, conserved motif, evolutionary relationship and expression patterns, etc., and the fluorescence quantitative PCR was used to determine the expression levels under abscisic acid, high temperature, low temperature and epidemic stress. The results showed that two Whirly genes (CaWHY1 and CaWHY2) were identified from the pepper variety CM334. Compared with the physicochemical properties of other species, Whirly, the CaWHY1 and CaWHY2 have little difference in structure and similarity.The CaWHY2 gene had exactly the same genetic structure with the SlWHY2 gene of tomato, which were clustered together in phylogenetic tree. CaWHY1 and SlWHY1 were also clustered together, but their genetic structures were different, indicating that the structure of the Whirly protein is conserved during the evolution.The expression pattern analysis showed that both CaWHY1 and CaWHY2 could express in pepper CM334, but their expression levels were different in different tissues and at different fruit development stages. CaWHY1 and CaWHY2 were induced to different degrees under different stress treatments, among which CaWHY1 was obviously induced by epidemic diseases. The expression of CaWHY2 under low temperature and abscisic acid stress showed an opposite trend. Above results suggested that, Whirly gene family played a regulatory role in pepper growth and development, and also played a certaun role in various stress.
2019 Vol. 34 (2): 72-78 [Abstract] ( 42 ) 全文: HTML   PDF (1148 KB)  ( 13 )
79 Cloning and Expression Analysis of 4-coumarate CoA Ligase Gene in Walnut Endocarp
GUO Yongcui, QIN Jiangnan, ZHU Ling, ZHANG Rui
DOI: 10.7668/hbnxb.201751031
In order to study the mechanism of Xinjiang walnut dew, the germplasm Wen 138 of bared-nut walnut was taken as the research object, and the mutant Zhipi walnut was used as the control. The 4CL gene was cloned by RT-PCR and analyzed by bioinformatics method. Fluorescence quantitative PCR was used to study the relative expression of 4CL gene in the process of walnut hardening, and the expression characteristics of 4CL in the development of hard shell walnut and the endocarp of bared-nut walnut were studied. The results showed that the cDNA sequence in WJ-4CL gene contained a 552 bp ORF, encoding 184 amino acids, with the molecular weight of 20.10 ku and isoelectric point of 5.83. The cDNA sequence in ZJ-4CL gene contained a 453 bp ORF, encoding 151 amino acids, and the molecular weight and isoelectric point were 16.52 ku and 9.35, respectively. The phylogenetic tree indicated that Wen 138 and Zhipi were clustered together, and their genetic relationship was relatively close. Real-time fluorescent quantitative PCR analysis indicated that relative expression of WJ-4CL gene in the 100 d after flowering was 117 times that in the 51 d after flowering, while the relative expression of ZJ-4CL gene in the 86 d after flowering was 205 times that in the 51 d after flowering, which preliminarily certified that the expression of 4CL gene was different in different development stages in the two walnut species. The results proved that the 4CL gene participated in the development of walnut endocarp harden processing and affected the synthesis of lignin in the endocarp, resulting in the hypoplasia of walnut endocarp and bared-nut.
2019 Vol. 34 (2): 79-86 [Abstract] ( 29 ) 全文: HTML   PDF (1478 KB)  ( 10 )
87 Functional Analysis of PH-START1 in Regulating Growth and Development of Arabidopsis thaliana
ZHAO Yazhuo, WANG Xin, FENG Jiajia, SUN Dandan, WANG Fengru, DONG Jingao
DOI: 10.7668/hbnxb.201751132
In order to clarify the function of PH-START1 (At2g28320) in regulating the growth and development of Arabidopsis, the expression of PH-START1 in different organs of Arabidopsis during different growth periods was analyzed by Real-time PCR, and the space-time expression characteristics of PH-START1 was determined. The PH-START1 function-obtaining and function-missing transgenic Arabidopsis were created and their phenotypes were analyzed. The biological function of PH-START1 in regulating the growth and development of Arabidopsis was elucidated. The Real-time PCR results showed that the expression of PH-START1 gene presented in the roots, leaves, flowers and siliques, and the highest expression level of PH-START1 was found in roots at seedling stage. The highest expression of the gene was in the 6th rosette leaf, then gradually decreased as the development time extended. In the development period of flowers, the expression of PH-START1 increased gradually with the development time of flowers. The highest expression of PH-START1 in kernels presented in 3-5 d after pollination, and then gradually decreased, but the expression of PH-START1 increased slightly in 9 d kernels after pollination. The highest expression of PH-START1 was in stamens, followed by petals and sepals, and the least in pistil. In order to analyze the function of PH-START1 in regulating the growth and development of Arabidopsis, the phenotype of the gain-of-function (overexpression, OE) and deletion (T-DNA insertion, ph-start1) transgenic Arabidopsis of PH-START1 was observed. The vegetative growth of PH-START1 overexpressing Arabidopsis was significantly inhibited, presenting shorter plant, weaker stems, smaller leaves and less roots. This suggested that PH-STARTI had a negative regulatory role in the growth and development of Arabidopsis, which provided a theoretical basis for elucidating and promoting the plant growth and development.
2019 Vol. 34 (2): 87-94 [Abstract] ( 26 ) 全文: HTML   PDF (1320 KB)  ( 15 )
95 Partial Virus Gene Sequence and Real-time Quantitative PCR Analysis of Tomato after Mixed Infection of ToCV and TYLCV
SONG Jian, XUE Jun, JIN Fengmei, SUN Haibo, ZHANG Yue, WANG Shu, FAN Huan, ZHOU Xiangming, CHEN Rui
DOI: 10.7668/hbnxb.201751035
There exists the compound infection of Tomato chlorosis virus (ToCV) and Tomato yellow leaf curl virus (TYLCV), and the incidence of the two diseases presents a tread of increase in recent years, which becomes a new threat to tomato farming. In order to study the interaction and variation of the two virus genes after single and mixed infection, the molecular identification and sequence analysis of the CP gene and the HSP gene of ToCV and the gene of TYLCV occurred in Tianjin were carried out. The results showed that, compared with the single infection samples, 6 base mutations of TYLCV were found in mixed infection samples, i.e. 73rd A→C, 92nd A→G, 347th C→T, 1 209th C→T, 1 618th A→G and 2 107th A→T, among which the 73rd and 92nd mutations were not in the coding region and the other four were located in the ORF box with 3 missense mutations and one samesense mutation(1 209th). There was also one samesense mutation in the CP gene of ToCV. There were 4 base mutations in HSP gene of ToCV, among which the 826th T→C and 1 166th G→A were samesense mutations, while the 1 395th A→G and 1 630th A→G were missense mutations. The accumulation amount of TYLCV and ToCV in tomato samples after single infection and mixed infection were analyzed by RT-qPCR. The results showed that the accumulations of TYLCV and ToCV had no significant difference between single infection and mixed infection. In the mixed infection samples, the copy of TYLCV was about 262-436 times that of ToCV.
2019 Vol. 34 (2): 95-102 [Abstract] ( 49 ) 全文: HTML   PDF (1188 KB)  ( 28 )
103 Reaction and Resistant Gene Loci Detection of Different Chinese Cabbage Varieties to Different Plasmodiophora brassicae Strains
WEN Juanjuan, WANG Chaonan, HUA Deping, ZHANG Hong, XU Yingli, ZHANG Bin
DOI: 10.7668/hbnxb.201751322
To clarify the resistant relationship between physiological races and Chinese cabbage(Brassica rapa L.ssp. pekinensis) variety, physiological races 4, 7, 10 and 11 were inoculated by injecting. The disease resistance of the 25 Chinese cabbage and CR markers were detected. The results showed that 8 cultivars such as Shandiwang 2 were resistance to only 4 physiological races; 7 cultivars such as Degao CR117 were susceptible to only 4 physiological races; 4 cultivars (including C1) were susceptible to 2 physiological races, and 6 cultivars (including Huakang 301) were only susceptible to 4 physiological race. All materials contained Crr1, CRk was detected in samples except the Degao CR Tiejia 1 and the Dadi CR118, the disease resistance locus CRa and CRb were detected in 21 and 16 samples respectively, 11 cultivars (including Degao CR117) contained Crr2 resistance locus, Crr3 resistance locus was detected only in Jingchun CR3, CRc resistance locus was contained in Xingguan and CR75.In short,different Chinese cabbage materials have different resistance to the same physiological race,and the same material responds differently to different physiological races. The physiological race No.4 was the most serious, followed by the physiological race No.7. The results of molecular marker identification showed that the resistance sites of Chinese cabbage materials were not necessarily resistant to disease. The resistance of Crr1 had little effect on material resistance.
2019 Vol. 34 (2): 103-109 [Abstract] ( 30 ) 全文: HTML   PDF (961 KB)  ( 20 )
110 KASP Assays of Disease Resistance Genes from 130 Watermelon Varieties and Germplasms in Hebei Province
ZHANG Jingjing, ZHANG Haiying, PAN Xiuqing, XU Yong, REN Yi, GAO Xiurui, LI Bing, SHI Yufan, DANG Jige, YANG Mingzhu, WU Yanrong
DOI: 10.7668/hbnxb.201751582
This study aimed to screen the watermelon varieties and germplasms resistant to fusarium wilt, anthracnose and powdery mildew in Hebei Province, and analyze the related resistance genes, which could provide a reference for breeding disease-resistant varieties. The modified CTAB method was used to extract the DNA from watermelon bud, and the DNA concentration was determined by Ultramicro spectrophotometer. After the DNA was diluted to 2-10 ng/μL, they were detected by KASP. The molecular markers of resistance to fusarium wilt, anthracnose and powdery mildew of watermelon were developed by XU Yong's team. The resistant genes related to fusarium wilt, anthracnose and powdery mildew of watermelon were identified by high-throughput KASP markers. Totally 130 watermelon materials were tested, including varieties or advantaging combinations (Xingyan No.7, Meijia, Meisheng, Guifei, 17-11 and so on) that have been approved or will be approved in Hebei Province in recent years, and some excellent germplasms (Huazaolü,JB-1,JB-3,901xin and so on). 3Kang302 was used as the disease-resistant control and GBZG as the susceptible control. The result showed that 33 materials contained fusarium wilt resistance gene Fon-1, 19 contained anthracnose resistance gene AR1, 7 contained powdery mildew resistance gene PM1, 5 contained Fon-1 and AR1, 1 contained AR1 and PM1, and 1 contained all three resistance genes(Fon-1, AR1 and PM1). According to the result of KASP, the materials were clustered, and divided into 4 categories:12 materials resistant to powdery mildew without detection signal or with heterozygous resistance, 67 resistant to powdery mildew or susceptible to at least two diseases, 38 resistant to fusarium wilt or anthracnose, and 13 resistant to fusarium wilt or anthracnose or powdery mildew without detection signal or with heterozygous resistance.
2019 Vol. 34 (2): 110-116 [Abstract] ( 53 ) 全文: HTML   PDF (1045 KB)  ( 26 )
117 Inheritance Model Analysis of Adult Plant Resistance Gene in Qinghai Spring Wheat Cultivar Gaoyuan 363 to Stripe Rust
DOI: 10.7668/hbnxb.201751235
In order to study the genetic rule of resistance to stripe rust in Qinghai spring wheat cultivar Gaoyuan 363, Qinghai spring wheat adult stage stripe rust resistance cultivar Gaoyuan 363 was crossed with the susceptible spring wheat cultivar Taichung 29 (T29) to produce the F2:3 segregating populations. The stripe rust resistance was identified separately in Xining and Huzhu for two years. The inheritance effects of stripe rust resistance in Gaoyuan 363/T29 F2 populations was analyzed by the method of single generation of joint segregation analysis using mixed inheritance model of major gene plus polygene. Frequency distributions results of Gaoyuan 363/T29 F2:3 populations the infection type and disease severity data showed that:tested on two places, Xining and Huzhu, and in two years for Gaoyuan 363/T29 F2:3 populations, the disease severity and infection type presented a bimodal distribution, which was uncontinuous, but was continuous in some parts. It preliminarily indicated that stripe rust resistance of Gaoyuan 363 was jointly controlled by major gene and minor genes; the result of inheritance model analysis showed that, for Gaoyuan 363 tested in both environments, whether using the disease severity or the infection type data, the most fitted genetic model for stripe rust resistance was controlled by two major genes and partially by minor genes, and the two major gene interactions (C-1:2MG-ADI additive-major-epistatic interaction,C-5:2MG-AED complete dominance effect,C-6:2MG-EEAD equal dominance effect) were different.
2019 Vol. 34 (2): 117-123 [Abstract] ( 30 ) 全文: HTML   PDF (985 KB)  ( 10 )
       Tillage and Cultivation·Physiology and Biochemistry
124 Response of Dry Matter Accumulation and Translocation to Planting Pattern and Planting Density in Spring Maize
LUO Fang, YANG Hengshan, ZHANG Yuqin, HE Dongdong, LIU Zhixiong
DOI: 10.7668/hbnxb.201751066
The maize variety Nonghua 101 was used as test material,to study the response of dry matter accumulation and translocation to planting patterns and planting densities of spring maize, including 2 planting patterns:wide-narrow row (80 cm+40 cm,KZ) and equal row spacing (60 cm, CK), and 3 planting densities. The dry matter accumulation amount at different stages of growth was measured, and the dry matter transfer amount and contribution rate to seeds were calculated. The results showed that the grain yields of maize in 3 densities of two experimental areas all showed KZ>CK, reaching to a significant level, and the increase rate ranging from 2.5% to 15.1%. The leaf area index was also KZ>CK. At the later growth stage, the decrease rate of leaf area index was CK>KZ, and the greater the density, the greater the decline. The SPAD value of leaves was KZ>CK at all stages, except for the leaves above ear in D1 and D2 densities at silking stage. The canopy transmittance was KZ>CK for all levels and in all densities, reaching to a significant level. The total dry matter accumulation and translocation amounts of stems and leaves showed KZ>CK in all three planting densities, reaching to a significant level. The contribution rate of stem-sheath and leaf to seeds was also KZ>CK at all densities, of which the highest came from the Daxingtun treatment with wide-narrow row planting pattern and D3 density. In Xiliaohe River Plain and similar areas, the planting pattern of wide-narrow row was beneficial to delaying the leaf senescence and increasing the dry matter accumulation and translocation under higher density during the late growth period, and might be an effective way to increase the density and the yield.
2019 Vol. 34 (2): 124-131 [Abstract] ( 52 ) 全文: HTML   PDF (1132 KB)  ( 62 )
132 Physiological Characteristics of Population and Its Response to Density in High-yield Silage Maize
SU Tianzeng, HOU Lexin, ZHANG Yuqiang, WANG Fangming, PENG Lei
DOI: 10.7668/hbnxb.20190064
Dajingjiu 26, a high yield and widely adaptive silage maize hybrid, was used as material to study the effects of different densities on the physiological characteristics and agronomic characters of high-yield population of silage maize in the West Liao-river Plain of Inner Mongolia, and six density levels were designed, i.e. 52 500, 60 000, 67 500, 75 000, 82 500 and 90 000 plants/ha, and the response of physiological characteristics and agronomic characters of high-yield population exceeding yield of 21 000 kg/ha to density was studied. The results showed that the biological yield of silage maize increased with the increase of planting density. The treatment of exceeding yield of 21 000 kg/ha were 67 500 and 75 000 plants/ha, reaching 21 751.1 and 22 551.1 kg/ha respectively. The yield of 52 500 plants/ha treatment was lowest, and the yield of 75 000 plants/ha treatment was 30.4% higher than that of 52 500 plants/ha treatment, reaching an extremely significant level. The population leaf area index of different planting densities presented reached the maximum value at silking stage. The two treatments of 67 500 and 75 000 plants/ha were highest as 5.19 and 5.32 respectively, retaining 3.68 and 3.85 at silage harvest stage. The photosynthetic potential increased with the increase of planting density, which was a positive effect, and the total photosynthetic potential was 326.51×104-384.37×104 (m2·d)/ha. The photosynthetic productivity of maize decreased with the increase of planting density, and the average photosynthetic productivity ranged from 6.50 to 7.64 g/(m2·d); The influence of planting density on the agronomic trait were in turn as follows:the fresh weight of single plant (17.6%), dry weight of single plant (15.5%), ear height (4.7%), staying green (3.0%), plant moisture content (2.1%), stem diameter (1.9%) and plant height (1.8%). The best planting density of silage maize exceeding yield of 21 000 kg/ha was 75 000 plants/ha.
2019 Vol. 34 (2): 132-137 [Abstract] ( 59 ) 全文: HTML   PDF (923 KB)  ( 62 )
138 Effects of Carbon Nanodots on Seedling Growth and Related Physiological and Biochemical Parameters in Peanut
MA Xingli, ZHAO Kunkun, ZHAO Pinyuan, LI Shanshan, MA Xiaoqiang, LI Zhongfeng, ZHANG Xingguo, YIN Dongmei
DOI: 10.7668/hbnxb.201751301
The effects of carbon nanodots with different concentrations on the seedling growth and related physiological and biochemical indexes of peanut were studied under the condition of indoor hydroponics. The results showed that the treatment of carbon nanodots had different promotion effects on seed germination, seedling growth, root activity and antioxidant enzyme activity. The germination potential, germination rate, main root length, seeding height, root dry mass, aboveground dry mass, root vigor, and the enzyme activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) of seedlings all showed the same trend of increasing first and then decreasing along with the increase of carbon nanodots concentration. In addition, carbon nanodots reduced the content of malondialdehyde (MDA), and increased the content of soluble sugar in peanut seedlings. Compared with the control, the treatment with 180 mg/L carbon nanodots had the best effect, in which the main root length, seeding height, root dry mass, aboveground dry mass, root fresh weight, aboveground fresh weight, SOD activity, CAT activity and root vigor increased by 36.84%, 46.24%, 31.03%, 47.03%, 55.93%, 42.22%, 27.73%, 36.81% and 4.5 times, respectively. The results indicated that suitable concentration of carbon nanodots could promote the seedling growth of peanut by regulating the physiological and biochemical behaviors.
2019 Vol. 34 (2): 138-144 [Abstract] ( 25 ) 全文: HTML   PDF (1029 KB)  ( 33 )
145 Physiological Response of Flax Seedlings with Different Drought-resistances to Drought Stress
WU Ruixiang, YANG Jianchun, WANG Liqin, GUO Xiujuan
DOI: 10.7668/hbnxb.201751272
Aiming to elucidate the physiological response mechanism of different drought resistant flax strains to drought stress. In a pot culture, four flax varieties (Jinya 7, Jinya 10, Jinya 11 and E051-20) with different types of drought resistance were used as the materials to study the effects of drought stress on the growth, membrane lipid peroxidation and osmotic adjustment substance content of flax seedlings. The results showed that, with the increase of drought stress and the length of stress time, the plant height, stem diameter, biomass and leaf relative water content of the plant decreased significantly, and the strong drought-resistant varieties decreased significantly less than the weak drought-resistant varieties, which was in turn:Jinya 7 < Jinya 10 < Jinya 11 < E051-20. At the same time, the MDA content, membrane relative permeability and proline content increased significantly, and the accumulation of proline content in resistant varieties was significantly higher than in less resistant varieties, with the order of Jinya 7 > Jinya 10 > Jinya 11 > E051-20. The malondialdehyde and plasma membrane permeability in weak resistant varieties increased more than in strong resistant varieties, and the amount of increase in turn was Jinya 7 < Jinya 10 < Jinya 11 < E051-20. The content of soluble protein was relatively stable. POD enzyme activity generally showed an upward trend, and the increase range of varieties with strong drought resistance was significantly higher than those with weak resistance. The correlation analysis showed that the plant height and stem diameter had very significantly positive correlation with the biomass, but had extremely significantly negative correlation with the contents of MDA and proline. It suggested that the biomass, MDA content and proline content could be used as the indexes to judge the drought resistance of flax seedlings.
2019 Vol. 34 (2): 145-153 [Abstract] ( 36 ) 全文: HTML   PDF (1441 KB)  ( 46 )
       Environment·Plant Protection
154 Effects of N Forms on Physiological Characteristics and Root Growth of Rice Seedling in Condition of Partial Root Water Stress
GAO Zhihong, CHEN Xiaoyuan, ZENG Yue
DOI: 10.7668/hbnxb.201751056
To study the response of rice Jinyou 402 seedling to nitrogen forms under partial root drying condition, a hydroponic experiment simulated by PEG was conducted using the method of nutrient solution culture. The trials had three water conditions:non-water stress, partial root drying, and all root water stress, and three nitrogen forms:NH4+-N/NO3--N of 0/100,50/50,100/0). Nitrogen absorption and accumulation, photosynthetic rate, transpiration rate, stomatal conductance, root length, root surface area and root volume of rice were determined. The results showed that under partial root drying condition, supplying both NH4+-N and NO3--N were most beneficial to nitrogen absorption and accumulation of rice seedling; The nitrogen absorption and accumulation were the minimum when NO3--N was supplied in two sides of root; The photosynthetic rate of rice, supplied with NH4+-N and NO3--N, were not affected obviously, but the transpiration rate and stomatal conductance declined significantly under partial root drying condition, compared to the rice with no water stress. The photosynthetic rate and stomatal conductance of rice supplied with NH4+-N and NO3--N in two sides of root were the highest among different nitrogen forms, and the lowest supplied with single NO3--N. WUE of rice supplied with NH4+-N and NO3--N was the biggest under the condition of partial root water stress. In condition of partial root drying, root length, root surface area, and root volume of most rices supplied with NH4+-N and NO3--N were all greater than those of rice supplied with single NH4+-N or NO3--N, and there were no significant difference between two sides of root. Furthermore, root length, root surface area, and root volume of rice subjected to partial root water stress environment were all greater than those with no water stress treatments, except for rice supplied with only NO3--N in two sides of root. This research proved that the interaction of partial root drying with nitrogen forms could promoted the water and nitrogen absorption of rice seedlings.
2019 Vol. 34 (2): 154-161 [Abstract] ( 38 ) 全文: HTML   PDF (1010 KB)  ( 37 )
162 Effect of Different Planting Methods for Increasing Seedlings to Save Nitrogen on Dry Matter Accumulation and Distribution in Late Rice of Double-cropping Rice
TANG Zhiwei, LONG Wenfei, DAI Wei, FU Zhiqiang
DOI: 10.7668/hbnxb.201750963
The effect of different planting methods for increasing seedlings to save nitrogen on the yield of double cropping rice was explored under the mode of water-saving cultivation. In 2016, a comparative trial on planting methods of artificial transplanting, machine-transplanted seeding and seeding slinging were conducted in the Ladder ridge paddy area of Lengshuitan District, Hunan Province. The results showed that the proportion of dry matter accumulation per plant at different growth stages of rice was 43.99%-71.71% from tillering stage to heading stage, 12.68%-28.17% from milk ripening stage to mature stage, 11.41%-25.36% from sowing date to tillering stage, and 4.20%-11.01% from heading stage to milk ripening stage. The correlation coefficient between dry matter accumulation and yield per plant was 0.77 from milk ripening to maturity, 0.38 from tillering to heading, 0.12 from heading to ripening, and-0.18 from sowing to tillering period. The stem and sheath per plant, and the leaf biomass showed a trend of increasing first and then decreasing, reaching the highest value at the heading stage. The proportion of total biomass per plant showed a downward trend, and the proportion of panicle biomass increased gradually. The proportion of stem and sheath biomass to total biomass of rice at each growth stage was 51.04%-59.69% at tillering stage, 46.03%-50.68% at heading stage, 31.82%-42.15% at milk ripening stage, and 20.97%-27.93% at maturity stage. The ratio of leaf biomass to total biomass at each growth stage was 40.31%-48.96% at tillering stage, 25.45%-28.53% at heading stage, 17.05%-20.15% at milk ripening stage, and 11.93%-14.33% at mature stage. In all treatments, the amount and proportion of dry matter accumulation was highest from tillering to heading stage, and the lowest from heading to milking stage. From sowing to tillering stage, the ratio of dry matter accumulation was the highest in the model of late rice no-tillage machine transplanting and machine harvesting+increasing seedlings to save nitrogen. From milky to mature stage, the highest proportion of dry matter accumulation came from the mode of late rice no-tillage artificial transplanting and artificial harvesting+increasing seedlings to save nitrogen.
2019 Vol. 34 (2): 162-170 [Abstract] ( 75 ) 全文: HTML   PDF (974 KB)  ( 36 )
171 Studies on Nitrogen Distribution Characteristics of Capsicum Organs and Matrix Nitrogen Transportation Law by 15N Isotope Tracing Technique
CAO Chaoqun, ZHANG Guobin, HU Linli, QIANG Haoran, MA Guoli, DU Miaoxin, LI Yutong
DOI: 10.7668/hbnxb.201751018
In order to explore the migration and accumulation of nitrogen fertilizer in the culture substrate of the greenhouse, and its absorption and distribution characteristics in various organs of pepper plants. Pepper (variety:Longjiao No.10) was used as the test material, and K15NO3 was used to mark K15NO3 in the depth of 5-10 cm and 15-20 cm, and set the lower limit of 60% (W60) and 80% (W80). The biomass of pepper grown in the greenhouse and the absorption and distribution of nitrogen in various organs of pepper and the migration of nitrogen in the culture substrate were studied. The results showed that the 60% minimum irrigation limit significantly increased the total biomass and nitrogen uptake of pepper plants. In the spatial distribution, the deeper the 15N mark fertilization depth, the lower the utilization rate of the pepper plant to 15N. In spatial distribution, the deeper the 15N labeled fertilization depth, the lower the 15N utilization rate of pepper plants. Meanwhile, the accumulation of 15N in the basal layer (0-20 cm) also decreased significantly, increased the amount of loss significantly. Under 60% irrigation water limit, the loss of 15N in the matrix was less than that under 80% irrigation condition. At this point, the amount of 15N migration downward at 15-20 cm of the matrix layer was reduced, and the absorption and utilization of total nitrogen by various organs of pepper plants under the condition of 60% lower irrigation limit was increased. Therefore, W60F5 treatment could increase the total biomass and nitrogen uptake of pepper plants, and reduced the downward nitrogen transport of the matrix, which was beneficial to the better absorption and utilization of pepper plants. The bioaccumulation and nitrogen uptake of various organs of pepper plants were leaf > fruit > stem.
2019 Vol. 34 (2): 171-177 [Abstract] ( 32 ) 全文: HTML   PDF (984 KB)  ( 23 )
178 Nitrogen Absorption of Winter Wheat Influenced by Combined Biofertilizer Replace Chemical Fertilizer
LIU Yuhui, TIAN Xiuping, ZHANG Qingwen, ZHANG Aiping, LIU Xingren, YANG Zhengli
DOI: 10.7668/hbnxb.201751359
Compound biofertilizers has been identified as an alternative to chemical fertilizer for increasing the soil fertility and crop production in sustainable farming. Nitrogen plays a vital role in the growth and yield of winter wheat, and is one of the main nutrients for water pollution. The use of biofertilizer can not only increase the plant biomass but also improve the total plant N assimilation. To reduce the agricultural source pollution load, a field experiment with five treatments was conducted by reducing the application of chemical fertilizer, and combined with biofertilizers or organic fertilizer to study the overground nitrogen uptake and utilization, and the grain yield of winter wheat in Binzhou, Shandong Province. The five treatments were:CK (N 200 kg/ha, P2O5 120 kg/ha, full dose), B1N2P2 (biofertilizer 1 7.5 kg/ha, N and P2O5 75% of the full dose), B1ON1P1 (biofertilizer 1 7.5 kg/ha, organic fertilizer 3 t/ha, N and P2O5 50% of the full dose), B2N2P2 (biofertilizer 2 7.5 kg/ha, N and P2O5 75% of the full dose); and B2ON1P1 (biofertilizer 2 7.5 kg/ha, organic fertilizer 3 t/ha, N and P2O5 50% of the full dose). The results showed that the application of biofertilizers could increase the overground nitrogen uptake amount of winter wheat at jointing and filling and maturing stages. The use of biofertilizers plus 25% reduction of chemical fertilizer could increase the overground nitrogen uptake rate and leaf SPAD value at jointing and filling stages, and also raise the yield and the rate of nitrogen allocation to grain. The nitrogen uptake efficiency of B2N2P2 was higher than those of biofertilizer 1 and CK. In the harvest time, B2N2P2 had the highest overground nitrogen uptake amount, grain nitrogen uptake amount and yield. In conclusion, the application of biofertilizers plus 25% reduction of chemical fertilizers could promote the nitrogen uptake, and increase the nitrogen harvest index, nitrogen uptake efficiency and nitrogen partial factor productivity of winter wheat, compared with CK.
2019 Vol. 34 (2): 178-186 [Abstract] ( 33 ) 全文: HTML   PDF (1310 KB)  ( 37 )
187 Effect of K Application Level on Biomass, K Absorption and Quality of Flue-cured Tobacco in Sand Mud Soil
ZHANG Shirong, WANG Jun, LIN Changhua, DING Xiaodong
DOI: 10.7668/hbnxb.201751060
Under the optimization of C/N with organic fertilizer and N fertilizer, a field experiment was carried out to study the effect of potassium and the ratio of basic/dressing fertilizer on biomass, potassium uptake, soil available potassium content and aroma quality of tobacco. Split-plot experiment design was adopted. The main treatment was 3 K level:K1 (120.0 kg/ha),K2 (240.0 kg/ha) and K3 (360.0 kg/ha);and the subsidiary treatment was the ratio of basic/dressing K fertilizer:S1(7:3),S2(5:5) and S3(3:7). The results showed that the biomass of tobacco leaves increased with the increase of potassium application, while it decreased with the increase of potassium dressing ratio in the same K level in the fast growth and mature period. Under the K2 and K3 level, the leaf potassium content was lower in the S1 treatment than in S2 and S3 treatments, indicating that the increase of K dressing percentage was conducive to the transfer of potassium ions from lower leaves to middle and upper leaves. With the increase of potassium application, the content of available and slowly available potassium in rhizosphere soil increased. Under K2 level, the available potassium of soil reached to maximum at fast growth and bud stages, and was lower in S3 treatment than in S1 and S2 treatments at mature stage. Under K3 level, the available potassium in rhizosphere soil had no significant difference among the four growth stages, in which that of S3 treatment > S1 treatment > S2 treatment. Compared with other treatments, the contents of total nitrogen, total nicotine and protein in tobacco leaves were significantly higher in S3 treatment under K2 level, and the ratio of sugar/alkali and ratio of nitrogen/alkali were improved. The neutral aroma components had no significant difference in upper leaves under K1 level, but it improved significantly in S3 treatment under K2 and K3 levels, especially for S3 treatment under the K2 level, in which the quality, quantity and concentration of aroma in tobacco leaves were the best. It was concluded that under the optimization conditions of organic fertilizer C/N, 240.0 kg/ha potassium fertilizer was recommended in the sand mud soil of Southern China, and the basic fertilizer was 72.0 kg/ha and the dressing one was 168.0 kg/ha (the ratio of base to dressing was 3:7), which could effectively improve the potassium content and quality of tobacco leaves.
2019 Vol. 34 (2): 187-197 [Abstract] ( 28 ) 全文: HTML   PDF (1247 KB)  ( 33 )
198 Effect of Biochar and Nitrogen Application Rate on Growth and Water-Nitrogen Use Efficiency of Tomato
LIU Yanni, LÜ Haofeng, ZHAO Yiming, LIN Shan
DOI: 10.7668/hbnxb.201751047
To explore whether the biochar application could reduce the negative effect of excessive nitrogen fertilization on tomato growth, a pot experiment was carried out using randomized block design with two factors (application rates of nitrogen and biochar) under three levels. The application rates were 100, 200,400 mg/kg of nitrogen, and 0, 2.5, 5 g/kg of biochar, respectively. The dry weight and fresh weight of stems, leaves and fruits of tomato, the amount of irrigation water and the water-nitrogen use efficiency were measured and calculated. The results demonstrated that the dry weight of stems and leaves and the accumulated irrigation water amount decreased significantly in the treatment of high nitrogen rate, compared with the treatment of low nitrogen rate.The nitrogen use efficiency decreased significantly along with the increase of N application rate. Compared with the treatment without biochar application, the use of biochar significantly increased the dry matter of leaves and fruit, the accumulated irrigation water amount and the water-nitrogen use efficiency. It suggested that the application of biochar could reduce the negative effect of excessive N fertilization on tomato growth.
2019 Vol. 34 (2): 198-204 [Abstract] ( 30 ) 全文: HTML   PDF (1058 KB)  ( 39 )
205 Studies on Diversity of Rhizosphere Microorganism in Quinoa Continuous Cropping Soil by High Throughput Sequencing
DONG Yanhui, YU Yufeng, WEN Xin, WANG Yixue, NIE Yuanjun, HOU Liyuan, LI Yali, LIU Jiang, REN Yuan, WANG Yuchuan, CAO Qiufen, WU Shenjie, WANG Bin, QIN Yongjun
DOI: 10.7668/hbnxb.201751218
In order to reveal the changes of bacterial community structure and diversity in quinoa continuous cropping soil, the V4 aera of 16S rRNA gene of bacteria from the quinoa rhizosphere soil with different treatments (planting for 1 a and for 2 a) was sequenced by high throughput sequencing. The results showed that the abundance and diversity index of the bacterial community in rhizosphere soil of planting quinoa for 1 a were higher than those of planting quinoa for 2 a, and the average values of Chao1, ACE and Shannon indexes increased by 16.4%, 22.9% and 2.3%, respectively. The taxonomic composition analysis of bacterial community showed that, after continuous cropping of quinoa, the bacterial diversity of Lentzea, Lysobacter, Mesorhizobium and Polaromonas increased, but the bacterial diversity of Mycobacterium, Luteimonas, Gemmatimonas and Planctomyces decreased. The analysis of functional prediction showed that the functional genes that code the membrane transport, the translation, copy and repair, the degradation and metabolism of exogenous substances and the biosynthesis and metabolism of polysaccharide decreased sigenificantly, and the functional genes coding the metabolism of nucleotide, steroids, cofactors and vitamin also decreased a little, but the functional genes of coding signal transduction and lipid metabolism increased in a small amount.
2019 Vol. 34 (2): 205-211 [Abstract] ( 65 ) 全文: HTML   PDF (1137 KB)  ( 41 )
212 Amino Acid Sequence Analysis of Mycoplasma hyopneumoniae P36, P46, P97 R1 and P146 in Slaughter Pigs
LI Runcheng, FANG Chao, QING Renke, GE Meng, ZHAO Dun, HU Yuli, YU Xinglong
DOI: 10.7668/hbnxb.201751207
To understand the genetic variation of Mycoplasma hyopneumoniae (M.hyo) epidemic strain, a conventional PCR was employed to amplify the fragments of M. hyo P36, P46, P97 R1 and P146 gene from the DNA mix of bronchoalveolar lavage fluid of 17 slaugher pigs, which had been tested positive to M.hyo, and the PCR products were sequenced directly and analyzed. The deduced amino acid sequences of P36 and P46 were conservative between M.hyo of 17 slagughter pig lung samples and those strains registered in GenBank, with a homology of more than 98.9%. Among the amino acid sequences, there were large variations in P97 R1 area and 2 repetitive sequence regions (R1, R2) of P146. P97 amino acid sequences R1 area of M.hyo from 11 slaughter pig samples and 5 strains registered in GenBank were composed of 14 or more tandem copies. It was composed of 9-11 tandem copies in three vaccine-related strains(168 strain, 168-L strain, J strain), four other strains registered in GenBank, and 4 strains from slaughter pigs. A few amino acid deficiency was observed in R1 area of P146 from 5 strains registered in GenBank (168 strain, 168-L strain, J strain, and two other strains) and 1 slaughter pig samples, and 3-9 amino acid deficiency was observed in R2 area of P146 from 11 strains (168-L strain, 168 strain, three other strains registered in GenBank, and 6 strains detected from slaughter pig). Consequently, antigen epitope prediction showed significant difference in P97 R1 area and two repetitive sequence regions (R1, R2) of P146. To the best of our knowledge, this study was the first preliminary investigation to M.hyo strain in slaughter pigs in Hunan province, and it could provide some significant data for understanding the prevalence of M.hyo strain in different areas and screening of vaccine candidate strains.
2019 Vol. 34 (2): 212-220 [Abstract] ( 26 ) 全文: HTML   PDF (2040 KB)  ( 25 )
221 Function of MYL3 Gene in Muscle Growth of Cattle
LIU Ruili, WU Lei, YUAN Wei, BAI Xuejin, LÜ Juanjuan, DONG Yajuan
DOI: 10.7668/hbnxb.201750982
The aim of this study was to investigate the effects of myosin light chain 3 (MLC1v or MYL3) on the regulation of skeletal muscle transcription, and to identify its functional relationship with muscle growth and development. MYL3 gene was cloned and its biological structure was analyzed by bioinformatics method. The expression of MYL3 gene in different tissues and at different stages was studied by means of fluorescence quantitative PCR technique. Immunohistochemistry showed that the expression locus of MYL3 gene were in the dorsal longissimus muscle. Western Blotting was used to study the expression of MYL3 in different tissues and at different stages. The results showed that the MYL3 gene of Black cattle (sequence number:NM_001076501.2) was cloned with a full-length reading frame of 600 bp, encoding 199 amino acids. Phylogenetic tree analysis showed that its homology was higher. Gene expression analysis showed the highest expression in the heart, followed by the longissimus muscle, and little in other tissues. The expression of MYL3 gene increased with the increase of age. The MYL3 protein mainly expressed in the myofilament of skeletal myofibrils, and increased with the age of the month at different stages. Presumably MYL3 might play an important role in skeletal muscle growth and development.
2019 Vol. 34 (2): 221-228 [Abstract] ( 27 ) 全文: HTML   PDF (1209 KB)  ( 21 )
229 Study of Aohan Fine Wool Sheep Hoxa5 Gene and BMPR1B Gene Interaction
ZHANG Mengyao, YANG Feng, LIU Jifeng, LIU Kaidong, HE Jianning, LIU Nan
DOI: 10.7668/hbnxb.201750743
The study aimed to identify the expression of Hoxa5 and BMPR1B gene plasmid and the interaction between the two genes was studied by means of expression changes. Firstly, a pair of primers were desigened by RNA extraction and referring to Hoxa5 and BMPR1B gene sequence information of Aohan fine wool sheep in GenBank, and the Hoxa5 and BMPR1B gene fragment were amplified by PCR method. The obtained Hoxa5 and BMPR1B gene were ligated into pEASYTM-T1 vector to construct pEASYTM-T1-Hoxa5 and pEASYTM-T1-BMPR1B recombinant plasmid and transformed into E.coli DH5α competent cell. The plasind was identified by restriction enzyme digedtion. The recombinant plasimd pcDNA3.1-Hoxa5 and pcDNA3.1-BMPR1B was constructed and transformed into E.coli DH5α competent cell. Then pcDNA3.1-Hoxa5 and pcDNA3.1-BMPR1B plasmid were cotransfection into fibroblasts, and qRT-PCR was used to detect the expression level of its gene. As the results showed that, pcDNA3.1-Hoxa5 and pcDNA3.1-BMPR1B plasmid cotransfection successfully and identified by enzyme and sequencing. The expression level of BMPR1B gene in fibroblasts was lower than in the control group and expression level of Hoxa5 gene in fibroblasts was higher than in the control group. The plasmid was constructed and cotransfection into fibroblasts successfully. The expression level of Hoxa5 gene increased significantly, and the expression of BMPR1B gene decreased. Therefore, Hoxa5 gene inhibited the expression of BMPR1B gene, and BMPR1B gene promoted the expression of Hoxa5 gene.These results would lay a foundation for the further research.
2019 Vol. 34 (2): 229-238 [Abstract] ( 31 ) 全文: HTML   PDF (1941 KB)  ( 30 )
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