Chromium(Cr)effects the growth of wheat plants(Triticum aestivum L.)badly.In this experiment,the effects of Cr toxicityon Chloraphyll content,root activity,malondialdehyde(MDA) content and antioxidative eniymes activity in seedlings were investigated to clarify different Cr resistibility in three wheat cultivars.The results showed:with the increase of Cr concentration,the germination potential,germination percentage,root length and shoot length increased first then declined.Under the same Cr concentration treatment,the characteristics of germination energy,germination percentage and shoot length presented Xinmai 19 >Aikang 58 > Yumai 18 the characteristics of root length presented Yumai 18 > Xinmai 19 >Aikang 581150 mg/L chromium treatment,chlorophyll content,root activities inhibitied heavy exposed to excess Cr.MDA content,SOD and POD content increased significantly in wheat shoots exposed to excess Cr.The SOD and POD activity also differed for the three cultivars.Cr stress significant promoted SOD and POD content in Yumai18 than Xinmai 19 and Aikang 58.
ZHANG Dai-jing
,
JIANG Li-na
,
SHAO Yun
,
LI Zhao-hu
,
LI Chun-xi
. Differential Responses in Germination and Antionxidant Enzymes of Three Wheat Cultivars to Chromium Stress[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(6)
: 69
-73
.
DOI: 10.7668/hbnxb.2009.06.014
[1] 张玉秀,柴团耀.植物重金属调节基因的分离和功能[M].北京:中国农业出版社,2006:1-2.
[2] Malkoc E,Nuhoglu Y,Abali Y.Cr adsorption by waste a corn of Quercus ithaburensis in fixed beds:prediction of breakthrough curves[J].Chem Eng,2006,119:61-68.
[3] Sandeep K,Joshi U N.Nitrogen metabolism as affected by hexavalent chromium in sorghum(Sorghum bicolor L.)[J].Environmental and Experimental Botany,2008,64(2):135-144.
[4] Adel M,Zayed,Norman T.Chromium in the environment:factors affecting biological remediation[J].Plant and Soil,2003,249:139-156.
[5] 郭峰,樊文华.土壤Hg、Cr和Pb单一污染对绿豆光合作用的影响[J].华北农学报,2009,24(1):26-30.
[6] Nalini P,Chandra P S.Effect of hevy metals CO_2+,Ni2+ and Cd2+ on gowth and metabolism of cabbage[J].Plant Science,2002,163:753-758.
[7] Sharma D C,Sharma C,Tripathi R D.Phototoxic lesions of chromium in maize[J].Chemosphere,2003,51(1):63-68.
[8] Valeria S,Marta L,Maria F P,et al.Species-dependent chromium accumulation,lipid peroxidation,and glutathione levels in germinating kiwifruit pollen under Cr(III)and Cr(VI)stress[J].Chemosphere,2008,73(7):1042-1048.
[9] 张宪政.植物叶绿素含量测定-丙酮乙醇浸提法[M].沈阳:辽宁农业出版社,1986(3):26-28.
[10] 张志良,瞿伟菁.植物生理学实验指导[M].北京:高等教育出版社,2004:39-41.
[11] 中国科学院上海植物生理研究所,上海市植物生理学会.现代植物生理学实验指南[M].北京:科学出版社,1999:305-306.
[12] Dhindsa R S,Dhinsa P P,Thorpe T A.Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation and decreased levels of superoxide dismutase and catalase[J].J Exp Bet,1981(32):193-101.
[13] Cakmak I,Horst W J.Effect of aluminium on lipid peroxidation,super oxide dismutase,catalase and peroxidase activities in root tips of soybean(Glycine max)[J].Physiol Plant,l991(83):463-468.
[14] Vajpayee R D,Tripathi M B,Singh S N.Chromium(VI)accumulation reduces chlorophyll biosynthesis,nitrate reductase activity and protein content in Nymphaea alba L.[J].Chemosphere,2000,41(7):1075-1082.
[15] 汪月霞,孙国荣,王建波,等.NaCl胁迫下星星草幼苗MDA含量与膜透性及叶绿素荧光参数之间的关系[J].生态学报,2006,26(1):122-129.
[16] 朱为民,丁海东,齐乃敏,等.Cd2+胁迫对番茄幼苗抗坏血酸-谷胱甘肽循环代谢的影响[J].华北农学报,2005,20(3):50-53.
[17] Cho U H,Seo N H.Oxidative stress in Arabidopsis thaliana exposed to cadmium is due to hydrogen peroxide accumulation[J].Plant Sci:2005,168,113-120.
[18] Murzaeva S V.Effect of heavy metals on wheat seedlings:activation of antioxidant enzymes[J].Applied Biochemistry and Microbiology,2004,1(40):98-103.
