越橘低温响应因子<i>VcICE1</i>的克隆和功能鉴定

doi:10.7668/hbnxb.201751537

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Abstract In order to excavate the role of blueberry cold response genes in cold stress and explore the regulation mechanism of cold stress response, a cold response factor gene VcICE1 (Inducer of CBF3 expression 1) from blueberry was isolated by PCR, and its expression pattern and response to cold stress were analyzed. The transgenic Arabidopsis thaliana were generated via Agrobacterium -mediated transformation, and the expression differences in response to cold stress were compared between the transgenic line and wild-type of Arabidopsis. The transient expression assays in tobacco leaves were carried out to test the transcriptional regulation of AtCBF3 by VcICE1. Sequence analysis showed that the open reading frame(ORF) of VcICE1 was 1 566 bp in length, encoding 522 amino acids. Protein structure analysis showed that VcICE1 contained a basic helix-loop-helix domain. The phylogenetic tree indicated that the blueberry VcICE1 exhibited the highest sequence similarity with the Arabidopsis thaliana AtICE1. Expression analysis showed that VcICE1 could express in roots, stems, young leaves, flowers and fruits, but the expression levels varied in different organs, the highest in young leaves and relatively low in fruits. The expression analysis result indicated that VcICE1 could be induced by cold stress treatment. The VcICE1 protein could activate the expression of AtCBF3 and favorably contributed to cold tolerance in transgenic Arabidopsis. VcICE1 could respond significantly to cold treatment. It was speculated that VcICE1 played a regulatory role in response to cold stress.

Key words： Blueberry Cold stress ICE1 Expression analysis Functional identification

Received: 10 March 2019

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	SONG Yang
	LIU Hongdi
	WANG Haibo
	ZHANG Hongjun
	LIU Fengzhi

Cite this article:

SONG Yang,LIU Hongdi,WANG Haibo, et al. Cloning and Functional Analysis of Cold Response Factor VcICE1 in Blueberry[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 1-8.doi: 10.7668/hbnxb.201751537.

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http://www.hbnxb.net/EN/10.7668/hbnxb.201751537 OR http://www.hbnxb.net/EN/Y2019/V34/I3/1

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