Acta Agriculturae Boreali-Sinica ›› 2022, Vol. 37 ›› Issue (2): 28-34. doi: 10.7668/hbnxb.20192653

Special Issue: Wheat Drought and water saving Biotechnology

• Crop Genetics & Breeding·Germplasm Resources·Biotechnology • Previous Articles     Next Articles

Cloning and Expression Analysis of TaSPP Gene in Wheat

JING Fanli1,2, ZHANG Peipei1, MIAO Yongping2, CHEN Tao2, LIU Yuan2, YANG Delong1,2   

  1. 1.Gansu Provincial Key Laboratory of Airdland Crop Science,Lanzhou 730070,China
    2.College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China
  • Received:2021-11-01 Published:2022-04-28


景凡丽1,2, 张沛沛1, 苗永平2, 陈涛2, 刘媛2, 杨德龙1,2   

  1. 1.甘肃省干旱生境作物学重点实验室,甘肃 兰州 730070
    2.甘肃农业大学 生命科学技术学院,甘肃 兰州 730070
  • 作者简介:
  • 基金资助:


In order to clarify the expression characteristics and biological functions of the sucrose phosphate phosphatase gene(SPP),and further understand the regulatory mechanism of SPP involved in sucrose biosynthesis.Herein,three TaSPP homologs,TaSPP-5A,TaSPP-5B and TaSPP-5D,located on the fifth homologous group,were cloned using the cDNA of wheat variety Jinmai 47 as the template.The physical and chemical properties,gene structure,cis-acting elements,phylogenetic tree and protein conserved domains of TaSPP were analyzed by the method of bioinformatics.The expression pattern of TaSPP was analyzed by qRT-PCR.The results showed that TaSPP-5A,TaSPP-5B and TaSPP-5D contained eight exons and seven introns.TaSPP-5A and TaSPP-5D encoded 422 amino acids,while TaSPP-5B encoded 413 amino acids.Phylogenetic analysis showed that TaSPP in wheat and its related species belonged to the same evolutionary branch with highly genetic similarity.The specific expression analysis showed that TaSPP genes were expressed in roots,stems,flag leaves,leaf sheaths,flower and seeds,whereas the higher expression levels were identified in flag leaves and stems.The expressions of TaSPP gene could be induced by ABA,PEG-6000,NaCl and IAA,indicating that TaSPP genes could play an essential role in abiotic stress tolerance in wheat.

Key words: Wheat, SPP, Cloning, Abiotic stress, Expression analysis



关键词: 小麦, 磷酸蔗糖磷酸酶基因, 克隆, 非生物胁迫, 表达分析