为了提高小麦幼胚体细胞再生频率,以河南省大面积推广的豫麦9号、豫麦18号和兰考906为研究对象,研究了不同浓度AgNO3对小麦幼胚胚性愈伤组织培养特性和草酸盐氧化酶活性的影响.结果表明:基因型间、不同AgNO_3浓度间小麦幼胚胚性愈伤组织诱导率、愈伤组织绿点率和草酸盐氧化酶活性差异均达到0.01显著水平,基因型间以豫麦18最高、兰考906最低;不同AgNO3浓度间以MS培养基上附加2.5～5.0 mg/L处理较高.不同浓度AgNO3条件下小麦幼胚胚性愈伤组织诱导率与草酸盐氧化酶活性相关系数为0.920,达0.01显著水平.可见,在小麦幼胚组织培养过程中可以加入Ag+提高再生频率.
何盛莲
,
雷振生
,
吴政卿
,
赵献林
,
方保停
,
杨会民
,
王美芳
,
何宁
,
杨攀
,
李巍
. AgNO3在小麦幼胚组织培养中的应用研究[J]. 华北农学报, 2009
, 24(6)
: 198
-201
.
DOI: 10.7668/hbnxb.2009.06.040
In order to improve the regeneration frequency of somatic embryos of wheat immature embryo,the effects of different concentration AgNO3 on someclone culture of immature embryo callus and oxalate oxidase activities of immature embryogenic callus were studied in this paper from three wheat cultivars (Yumai 18,Yumai 49 and Lankao 906) which were cultured with more areas in Henan province.The result indicated that the differences of induction frequency of emryogenic callus andgreen spot rates of callus and oxalate oxidase activities of immature embryogenic callus were significant at 0.01 level among different AgNO3concentrations or genotypes.It was highest in Yumai 18 and was lowest in Lankao 906 among different genotypes.It were highest in MS medium suppliedwith 2.55.0 mg/ L AgNO3 among different AgNO3 concentrations.The coefficient between induction frequency and oxalate oxidase activities of immature emryogenic callus in wheat under different AgNO3concentrations was0.920 and it was significant at 0.01 level.So,the regeneration frequency can be increased in someclone culture of wheat immature embryo supplied with Ag+.
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