两个大豆开花期QTL定位及对农艺性状的影响分析

王涛; 杨春燕; 赵青松; 闫龙; 肖付明; 智海剑; 张孟臣

doi:10.3969/j.issn.1000-7091.2013.02.012

华北农学报 >

2013 , Vol. 28 >Issue 2: 63 - 69

DOI: https://doi.org/10.3969/j.issn.1000-7091.2013.02.012

论文

两个大豆开花期QTL定位及对农艺性状的影响分析

王涛 ,
杨春燕 ,
赵青松 ,
闫龙 ,
肖付明 ,
智海剑 ,
张孟臣

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1. 国家大豆改良中心, 南京农业大学大豆研究所, 作物遗传与种质创新国家重点实验室, 江苏南京 210095;
2. 河北省农林科学院粮油作物研究所, 国家大豆改良中心石家庄分中心, 农业部黄淮海大豆生物学与遗传育种重点实验室, 河北省作物遗传育种重点实验室, 河北石家庄 050031;
3. 邯郸市农业科学院, 河北邯郸 056001

王涛(1985－)，男，河北邢台人，硕士，主要从事大豆分子数量遗传与育种研究。

收稿日期: 2012-01-08

网络出版日期: 2014-10-14

基金资助

国家“863”计划(2012AA101106);国家自然科学基金(31000719);国家转基因生物新品种培育重大专项(2011ZX08009-088B);国家现代农业产业技术体系(CARS-004-PS06)

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Two Quantitative Trait Loci for Flowing Time and Their Effect on Agronomic Traits in Soybean

WANG Tao ,
YANG Chun-yan ,
ZHAO Qing-song ,
YAN Long ,
XIAO Fu-ming ,
ZHI Hai-jian ,
ZHANG Meng-chen

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1. National Center for Soybean Improvement, Soybean Research Institude, Nanjing Agricultural University, National Key Laboratory for Cropgenetics andgermplasm Enhancement, Nanjing 210095, China;
2. Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang Branch Center of National Center for Soybean Improvement, North China Key Laboratory of Biology andgenetic Improvement of Soybean, Hebeigenetic Breeding Laboratory, Shijiazhuang 050035, China;
3. Handan Agriculture Academy of Scienges, Handan 056001, China

Received date: 2012-01-08

Online published: 2014-10-14

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摘要

以冀豆12为母本,冀nf 58为父本通过杂交构建包含175个家系的F9:11重组自交系群体为试验材料。采用134个SSR标记,利用Kosambi函数绘制了一张包含117个标记,长度为1 373.6 cM的遗传图谱。运用QTL Cartogra-pher v2.5软件的复合区间作图法（CIM）和QTL Network 2.0软件的基于混合线性模型的区间作图法（NWIM）同时检测到2个控制开花期性状的QTL。其中,qFTO-1定位在O连锁群标记区间Satt581～Sat₁90内,加性效应值分别为2.49和2.02,增效基因来自nf 58,贡献率最高为27.2%|qFTC2-1定位在C2连锁群标记区间Satt557～Sat₂51内,加性效应值分别为-3.50和-3.84,增效基因来自冀豆12,贡献率最高为55.6%。2个连锁群上QTL间存在加性×加性上位互作效应。效应值为-0.672 8,重组型大于亲本型。2个QTL对产量相关性状有显著影响,与表型相关分析结果的趋势一致。其中,qFTO-1平均提高单株产量2.62g,qFTC2-1平均提高单株产量2.26g。这些研究为分子标记辅助育种及分子克隆提供理论依据。

关键词： 大豆; 开花期; 相关分析; QTL; 上位互作效应

本文引用格式

王涛 , 杨春燕 , 赵青松 , 闫龙 , 肖付明 , 智海剑 , 张孟臣 . 两个大豆开花期QTL定位及对农艺性状的影响分析[J]. 华北农学报, 2013 , 28(2) : 63 -69 . DOI: 10.3969/j.issn.1000-7091.2013.02.012

Abstract

A cross of Jidou 12 and nf 58 was made to construct a recombinant inbred line of F9:11population which has 175 progenies as the experimental material. Agenetic linkage map of soybeangenome was constructed,which include 117 markers and covering 1 373. 6 cM. CIM method of QTL Cartographer v2. 5 software and NWIM method of QTL Network 2. 0 were used to identify quantitative trait loci and two QTLs were detected. qFTO-1 were located in the marker interval Satt581 ～ Sat_190 of O linkagegroup,additive value were 2. 49 and 2. 02,favorablegene from nf 58 and the highestgenetic contribution rate is 27. 2%. qFTC2-1 was located in the marker interval Satt557 ～ Sat_251 of C2 linkagegroup,additive value were-3. 50 and-3. 84,favorablegene from Jidou 12 and the highestgenetic contribution rate is 55. 6%. Additive×Additive epistatic effect was existent between the two QTLs. The value is-0.672 8 and the recombinant type is better than the parent. The two QTL effected on yield related

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