华北农学报 ›› 2020, Vol. 35 ›› Issue (3): 79-86. doi: 10.7668/hbnxb.20190471

所属专题: 油料作物

• 作物遗传育种·种质资源·生物技术 • 上一篇    下一篇

利用大豆四向重组自交系群体定位产量相关性状QTL

王艳殊1, 田雨1, 张佳楠1, 许世超1, 董全中2, 李文滨1, 李文霞1, 宁海龙1   

  1. 1. 东北农业大学 农学院, 大豆生物学教育部重点实验室, 农业部东北大豆生物学与遗传育种重点实验室, 黑龙江 哈尔滨 150030;
    2. 黑龙江省农业科学院 克山分院, 黑龙江 克山 161606
  • 收稿日期:2019-12-22 出版日期:2020-06-28
  • 通讯作者: 李文霞(1974-),女,黑龙江哈尔滨人,副教授,博士,硕士生导师,主要从事大豆遗传育种研究;宁海龙(1975-),男,黑龙江哈尔滨人,教授,博士,博士生导师,主要从事大豆遗传育种研究。
  • 作者简介:王艳殊(1990-),女,内蒙古赤峰人,硕士,主要从事作物遗传育种研究。
  • 基金资助:
    黑龙江省自然科学基金留学归国项目(LC2016010)

QTL Identification of Yield-related Traits Using Four-way Recombinant Inbred Population in Soybean

WANG Yanshu1, TIAN Yu1, ZHANG Jianan1, XU Shichao1, DONG Quanzhong2, LI Wenbin1, LI Wenxia1, NING Hailong1   

  1. 1. Agronomy College, Northeast Agricultural University, Key Laboratory of Soybean Biology, Ministry of Education, Key Laboratory of Soybean Biology and Breeding(Genetics), Ministry of Agriculture, Harbin 150030, China;
    2. Keshan Branch of Heilongjiang Academy of Agricultural Sciences, Keshan 161606, China
  • Received:2019-12-22 Published:2020-06-28

摘要: 为探明大豆产量与株高、主茎节数、单株荚数、单株粒数、百粒质量等产量构成因素间的相关性,定位控制这些性状的QTL进而提高大豆产量,以4个产量相关性状差异较大的大豆亲本配制双交组合(垦丰14×垦丰15)×(黑农48×垦丰19)衍生的包含160个株系的四向重组自交系群体(FW-RIL)为材料,在哈尔滨(2013-2015年)和克山(2013,2015年)种植,获得的株高、主茎节数、单株荚数、单株粒数、百粒质量的表型数据,结合已经构建的包含275个SSR标记的大豆遗传图谱对产量相关性状QTL进行定位。结果表明:在多个环境下重复稳定检测到产量相关性状的QTL 28个,其中10个株高QTL可解释表型变异率在3.20%~11.72%,3个主茎节数QTL可解释表型变异率分别为6.55%,5.70%,3.77%,9个单株荚数QTL可解释表型变异率在2.60%~11.25%,4个百粒质量QTL可解释表型变异率在3.83%~9.35%,2个单株粒数QTL可解释表型变异率分别为8.58%,7.52%;28个多环境重复检测到的产量性状QTL,其中16个QTL是本研究新检测到的,12个与国内外报道过的产量相关性状QTL位点一致,说明QTL检测准确率较高。利用分子标记遗传图谱,定位控制产量相关性状的QTL,为利用分子标记改良大豆产量潜力提供了有力手段。

关键词: 大豆, 产量, QTL定位, 四向重组自交系群体

Abstract: The purpose of this study was to explore the correlation between soybean yield and yield-related such as plant height, main stem node number, pod number per plant, seed number per plant and 100-seed weight and to localize QTLs for controlling these traits so as to improve soybean yield.This experiment, the four-way recombinant inbred line population(FW-RIL)with 160 lines derived from four soybean parents (Kenfeng 14×Kenfeng 15)×(Heinong 48×Kenfeng 19) with significant difference in yield-related characters was used as materials and planed in Harbin (2013-2015) and Keshan (2013,2015). Obtaining phenotypic data of plant height, main stem node number,pod number per plant, seed number per plant, 100-seed weight combined with constructed genetic map containing 275 SSR markers to map QTL for yield-related traits. Experimental results showed that 28 yield-related QTLs were detected repeatedly in multi-environments and 10 of them were plant height QTLs, phenotypic variation explained was between 3.20% and 11.72%. Three main stem node number QTLs, phenotypic variation explained was 6.55%,5.70%,3.77% respectively. Nine pod number per plant QTLs, phenotypic variation explained was between 2.60% and 11.25%. Four 100-seed weight QTLs, phenotypic variation explained was between 3.83% and 9.35%.Two seed number per plant QTLs, phenotypic variation explained was 8.58%,7.52% respectively.28 repeated detected yield traits QTLs were located,16 new yield-related QTLs were detected and 12 of them were consistent with the QTLs for yield-related traits reported at home and abroad, indicating a high accuracy of QTLs detection. Using molecular marker genetic map to locate QTLs for controlling yield-related traits provides a powerful means for using molecular marker to improve soybean yield potential.

Key words: Soybean, Yield, QTL mapping, Four-way recombinant inbred population

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引用本文

王艳殊, 田雨, 张佳楠, 许世超, 董全中, 李文滨, 李文霞, 宁海龙. 利用大豆四向重组自交系群体定位产量相关性状QTL[J]. 华北农学报, 2020, 35(3): 79-86. doi: 10.7668/hbnxb.20190471.

WANG Yanshu, TIAN Yu, ZHANG Jianan, XU Shichao, DONG Quanzhong, LI Wenbin, LI Wenxia, NING Hailong. QTL Identification of Yield-related Traits Using Four-way Recombinant Inbred Population in Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 79-86. doi: 10.7668/hbnxb.20190471.

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