水稻LRR-RLK基因<i>LP7</i>的克隆及表达分析

doi:10.7668/hbnxb.201751355

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摘要 LRR-RLK是类受体蛋白激酶RLK家族中最大的亚家族，在调控植物非生物胁迫等方面具有重要作用。为解析水稻LRR-RLK成员LP7（LOC_Os05g24010）在耐低磷胁迫中的作用，从水稻品种日本晴中克隆了LP7全长序列，分析其编码蛋白的氨基酸序列，研究其组织表达模式、亚细胞定位及低磷胁迫下的表达变化。结果表明，LP7基因全长2 832 bp，编码943个氨基酸，LP7蛋白具有典型的LRR-RLK成员特征，LP7蛋白与玉米中的同源蛋白NP_001131018同源性比较高，同源性高达77%。组织表达模式分析表明，LP7基因在根、茎、叶等组织中均表达，在叶中表达量最高。亚细胞定位结果表明，LP7蛋白定位于细胞膜上。实时荧光定量PCR分析表明，LP7基因受低磷胁迫诱导表达，其表达量较正常培养条件下增加15倍。初步推测该基因可能在水稻响应低磷胁迫中具有重要作用。

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	林法明
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	李光豪
	王代长
	杜长青
	赵全志

关键词 ：水稻；类受体蛋白激酶；低磷胁迫响应基因LP7；亚细胞定位；低磷胁迫

Abstract：LRR-RLK (Leucine-rich repeat receptor-like protein kinase) is the largest subfamily of the receptor-like protein kinase RLK family and plays an important role in regulating the plant abiotic stress. To understand the role of rice LRR-RLK member LP7 (LOC_Os05g24010) in resistance to low phosphorus stress, the full-length sequence of LP7 was cloned from rice variety Nipponbare, and its deduced amino acid sequence was analyzed to study the tissue expression pattern, subcellular localization and expression change under low phosphorus stress. The results showed that the LP7 gene was 2 832 bp in length and encoded 943 amino acids. The LP7 protein had the typical characteristics of LRR-RLK membership. The LP7 protein had high homology with the homologous protein NP_001131018 of maize, reaching to 77%. Expression patterns showed that LP7 could express in roots, stems, leaves and other tissues, but the highest in leaves. Subcellular localization result indicated that the LP7 protein was localized on the cell membrane. Real-time quantitative PCR analysis showed that the LP7 gene was induced by low-phosphorus stress, and its expression level increased by 15 times, compared to normal culture condition. The results indicated that LP7 might have important functions in response to low phosphorus stress in rice.

Key words： Rice ; Receptor-like protein kinase ; Low phosphorus stress response gene LP7 ; Subcellular location ; Low phosphorus stress

收稿日期: 2019-01-11

PACS:	S511.03
	Q78

基金资助:河南农业大学高层次人才引进启动经费项目（30500561）

通讯作者: 杜长青(1984-),男,河南濮阳人,副教授,博士,硕士生导师,主要从事水稻生物化学与分子生物学研究。

作者简介: 林法明(1992-),男,河南濮阳人,在读硕士,主要从事水稻分子生物学研究。

引用本文:

林法明, 李珅, 王珂, 高俊峰, 李光豪, 王代长, 杜长青, 赵全志. 水稻LRR-RLK基因LP7的克隆及表达分析[J]. 华北农学报, 2019, 34(2): 19-24. doi: 10.7668/hbnxb.201751355.
LIN Faming, LI Shen, WANG Ke, GAO Junfeng, LI Guanghao, WANG Daichang, DU Changqing, ZHAO Quanzhi. Cloning and Expression of LRR-RLK Gene LP7 in Rice. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 19-24. doi: 10.7668/hbnxb.201751355.

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http://www.hbnxb.net/CN/10.7668/hbnxb.201751355 或 http://www.hbnxb.net/CN/Y2019/V34/I2/19

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