华北农学报 ›› 2019, Vol. 34 ›› Issue (1): 213-218. doi: 10.7668/hbnxb.201750779

所属专题: 玉米 油料作物

• 资源环境·植物保护 • 上一篇    下一篇

油菜素内酯提高玉米抗禾谷镰孢菌侵染的生理机制

张昊, 冯佳佳, 孙丹丹, 闫青地, 赵亚林, 王珊, 王平平, 赵亚卓, 王倩, 王凤茹, 董金皋   

  1. 河北农业大学 生命科学学院, 河北省植物生理和分子病理学重点实验室, 河北 保定 071001
  • 收稿日期:2018-11-26 出版日期:2019-02-28
  • 通讯作者: 王凤茹(1972-),女,河北景县人,教授,博士,主要从事植物生长发育调控研究。
  • 作者简介:张昊(1989-),男,河北行唐人,在读博士,主要从事植物生长发育调控研究。
  • 基金资助:
    研究生创新创业项目(2017ZD06;CXZZBS2018113);大学生创新创业项目(2018090);国家重点研发计划项目(2016YFD0300704);国家重点研发区域课题(2018YFD0200607)

The Physiological Mechanism of the Increase of the Infection of the Maize Resistance to the Fusarium graminearum

ZHANG Hao, FENG Jiajia, SUN Dandan, YAN Qingdi, ZHAO Yalin, WANG Shan, WANG Pingping, ZHAO Yazhuo, WANG Qian, WANG Fengru, DONG Jingao   

  1. College of Life Sciences, Hebei Agricultural University, Key Laboratory of Hebei Province for Plant Physiology and Molecular Plant Pathology, Baoding 071001, China
  • Received:2018-11-26 Published:2019-02-28

摘要: 为明确油菜素内酯(BR)增强玉米抗禾谷镰孢侵染的作用及机制,以玉米郑单958为试验材料,分设喷施BR和未喷施BR的处理,在玉米喷施BR 10 d后,接种禾谷镰孢菌,调查比较喷施BR后玉米感染禾谷镰孢菌的发病情况,确定油菜素内酯提高玉米抗禾谷镰孢侵染的确切作用,结果表明,在未喷施BR的自然生长条件下,在玉米上接种禾谷镰孢,发现茎秆表面有明显的病原菌沿接种针孔侵染扩散的痕迹,玉米茎剖面内髓组织黑褐色严重,几乎整个节间均已被病原菌侵染;但在喷施BR 10 d后再接种禾谷镰孢,玉米茎剖面内的髓组织被病原菌侵染面积明显减少,发病明显减轻,说明BR提高了玉米抗禾谷镰孢侵染的能力。用含BR的PDA培养基培养禾谷镰孢,与对照相比,禾谷镰孢发育未受到明显的影响。BR处理后,玉米叶片脯氨酸含量增加,Real-time PCR分析编码脯氨酸合成酶的基因ZmP5CS的表达量,发现其表达量确实受BR的诱导。同时BR处理后玉米叶片可溶性糖含量极显著增加,保护酶SOD和PAL活性显著增加,POD活性极显著增加。说明BR提高玉米自身抗逆性来提高抵抗禾谷镰孢侵染。油菜素内酯对禾谷镰孢的生长发育没有明显的影响;油菜素内酯提高玉米抗禾谷镰孢侵染的原因可能不是抑制禾谷镰孢的生长发育,而是通过提高玉米脯氨酸合成、保护酶活性、可溶性糖含量,从而提高玉米自身免疫能力。

关键词: 油菜素内酯, 禾谷镰孢, 生理机制, 玉米

Abstract: To clear the effect and mechanism of brassinosteroid (BR) on enhancing the maize resistance against the Fusarium graminearum infection, maize Zhengdan 958 was used as the experimental material, two treatments were spraying BR and unspraying BR on maize plants,10 days later after treatment processing, inoculated Fusarium graminearum spore on the stem,and then investigated and compared the maize incidence after treatment of spraying BR, determined the exact effect of BR on improving the maize resistance against the Fusarium graminearum infection. The results showed that under the natural condition without spraying BR, after inoculating Fusarium graminearum spore on maize stem, there were obvious traces of pathogen infection and spread along the inoculation needle on the stem surface, the medullary tissue was seriously dark brown, and almost all the nodes had been infected with pathogens. But the invasion area by the pathogen in intramedullary tissue was significantly reduced of the maize stem sprayed BR 10 days before inoculating Fusarium graminearum spore, and the morbidity was significantly reduced, this indicated that BR enhanced the ability of maize to resist the infection. The growth and development of Fusarium graminearum colony were not affected obviously when cultured in the PDA medium containing BR. After BR treatment, the content of proline in maize leaves increased, Real-time PCR analyzed the expression of gene ZmP5CS which encoded with proline synthase, and found that its expression was indeed induced by BR. At the same time, the content of soluble sugar in corn increased extremely significant, and the enzyme activity of protective enzyme SOD and PAL significantly increased and POD increased extremcly significant, it was shown that BR improved the resistance of maize to Fusarium graminearum infestation by raising the corn's own immunity. The conclusion was that BR treatment had no obvious effect on the growth and development of Fusarium graminearum colony, the reason for the increase of the maize resistance against the Fusarium graminearum infection was not the inhibition of BR on the growth and development of Fusarium graminearum, but by raising corn proline synthesis, protective enzyme activity, soluble sugar content and improve corn immune ability.

Key words: Brassinosteroid, Fusarium graminearum, Physiological mechanism, Maize

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张昊, 冯佳佳, 孙丹丹, 闫青地, 赵亚林, 王珊, 王平平, 赵亚卓, 王倩, 王凤茹, 董金皋. 油菜素内酯提高玉米抗禾谷镰孢菌侵染的生理机制[J]. 华北农学报, 2019, 34(1): 213-218. doi: 10.7668/hbnxb.201750779.

ZHANG Hao, FENG Jiajia, SUN Dandan, YAN Qingdi, ZHAO Yalin, WANG Shan, WANG Pingping, ZHAO Yazhuo, WANG Qian, WANG Fengru, DONG Jingao. The Physiological Mechanism of the Increase of the Infection of the Maize Resistance to the Fusarium graminearum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 213-218. doi: 10.7668/hbnxb.201750779.

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